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Literature summary extracted from

  • Campos, M.; Couture, C.; Hirata, I.Y.; Juliano, M.A.; Loisel, T.P.; Crine, P.; Juliano, L.; Boileau, G.; Carmona, A.K.
    Human recombinant endopeptidase PHEX has a strict S1' specificity for acidic residues and cleaves peptides derived from fibroblast growth factor-23 and matrix extracellular phosphoglycoprotein (2003), Biochem. J., 373, 271-279.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.B15 PHEX gene, i.e. phosphate-regulating gene with homologies to endopeptidase on the X-chromosome, expression as soluble secreted mutant form secPHEX, lacking residues 1-45, in LLC-PK1 porcine kidney cells, deletion mutant enzyme shows catalytic properties similar to the wild-type enzyme Homo sapiens

General Stability

EC Number General Stability Organism
3.4.24.B15 NaCl required for stability of the purified enzyme Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.B15 additional information no inhibition by thiorphan of wild-type and soluble enzyme form Homo sapiens
3.4.24.B15 NaCl 2fold lower kcat/Km, but required for stability Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.B15 0.0009
-
 o-aminobenzoyl-L134ELDSRQ-(2,4-dinitrophenyl)ethylene diamine recombinant soluble enzyme, pH 5.5, 37°C Homo sapiens
3.4.24.B15 0.0013
-
 o-aminobenzoyl-D124HLSDTSTQ-N-(2,4-dinitrophenyl)ethylene diamine recombinant soluble enzyme, pH 5.5, 37°C Homo sapiens
3.4.24.B15 0.0015
-
 o-aminobenzoyl-A110WLDSGVQ-N-(2,4-dinitrophenyl)ethylene diamine recombinant soluble enzyme, pH 5.5, 37°C Homo sapiens
3.4.24.B15 0.0017
-
 o-aminobenzoyl-R175RHTQSAEDDSERQ-N-(2,4-dinitrophenyl)ethylene diamine recombinant soluble enzyme, pH 5.5, 37°C Homo sapiens
3.4.24.B15 0.0024
-
 o-aminobenzoyl-G386SSDAAEQ-N-(2,4-dinitrophenyl)ethylene diamine recombinant soluble enzyme, pH 5.5, 37°C Homo sapiens
3.4.24.B15 0.003
-
 o-aminobenzoyl-GFSDYK-(2,4-dinitrophenyl)-OH pseudo-first-order conditions, recombinant soluble enzyme, pH 5.5, 37°C Homo sapiens
3.4.24.B15 0.003
-
 o-aminobenzoyl-R175RHTRSAEDDSERQ-N-(2,4-dinitrophenyl)ethylene diamine recombinant soluble enzyme, pH 5.5, 37°C Homo sapiens
3.4.24.B15 0.004
-
 o-aminobenzoyl-R76SEDAGFQ-N-(2,4-dinitrophenyl)ethylene diamine recombinant soluble enzyme, pH 5.5, 37°C Homo sapiens
3.4.24.B15 0.005
-
 o-aminobenzoyl-R506RDDSSEQ-N-(2,4-dinitrophenyl)ethylene diamine recombinant soluble enzyme, pH 5.5, 37°C Homo sapiens
3.4.24.B15 0.007
-
 o-aminobenzoyl-R441GLDNEIQ-N-(2,4-dinitrophenyl)ethylene diamine recombinant soluble enzyme, pH 5.5, 37°C Homo sapiens
3.4.24.B15 0.009
-
 o-aminobenzoyl-N122GYDVYHQ-N-(2,4-dinitrophenyl)ethylene diamine recombinant soluble enzyme, pH 5.5, 37°C Homo sapiens
3.4.24.B15 0.013
-
 o-aminobenzoyl-GFSDYQ-N-(2,4-dinitrophenyl)ethylene diamine pseudo-first-order conditions, recombinant soluble enzyme, pH 5.5, 37°C Homo sapiens
3.4.24.B15 0.013
-
 o-aminobenzoyl-G337SNDIMGQ-N-(2,4-dinitrophenyl)ethylene diamine recombinant soluble enzyme, pH 5.5, 37°C Homo sapiens
3.4.24.B15 0.015
-
 o-aminobenzoyl-T294HLDTKKQ-N-(2,4-dinitrophenyl)ethylene diamine recombinant soluble enzyme, pH 5.5, 37°C Homo sapiens
3.4.24.B15 0.017
-
 o-aminobenzoyl-L94MMDFRGQ-N-(2,4-dinitrophenyl)ethylene diamine recombinant soluble enzyme, pH 5.5, 37°C Homo sapiens
3.4.24.B15 0.022
-
 o-aminobenzoyl-S212AEDNSPQ-N-(2,4-dinitrophenyl)ethylene diamine recombinant soluble enzyme, pH 5.5, 37°C Homo sapiens
3.4.24.B15 0.032
-
 o-aminobenzoyl-S513SDSGSQ-N-(2,4-dinitrophenyl)ethylene diamine recombinant soluble enzyme, pH 5.5, 37°C Homo sapiens
3.4.24.B15 0.037
-
 o-aminobenzoyl-N449EMDSFNQ-N-(2,4-dinitrophenyl)ethylene diamine recombinant soluble enzyme, pH 5.5, 37°C Homo sapiens
3.4.24.B15 0.047
-
 o-aminobenzoyl-G197QRDSQAQ-N-(2,4-dinitrophenyl)ethylene diamine recombinant soluble enzyme, pH 5.5, 37°C Homo sapiens
3.4.24.B15 0.053
-
 o-aminobenzoyl-GFSDYK-(2,4-dinitrophenyl)-NH2 pseudo-first-order conditions, recombinant soluble enzyme, pH 5.5, 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.B15 membrane
-
 Homo sapiens 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.B15 protein + H2O Homo sapiens
-
 peptides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.B15 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.B15 recombinant soluble enzyme form from porcine kidney cells Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.24.B15 proteolytic degradation of proteins preference for an acidic residue at position P'1, especially for aspartic acid, glutamic acid gives 5fold lower activity Homo sapiens
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.B15 additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.B15 additional information no activity of wild-type and soluble enzyme form with o-aminobenzoyl-RL-N-(2,4-dinitrophenyl)ethylene diamine, no activity with o-aminobenzoyl-GFSEYK-(2,4-dinitrophenyl)-NH2 Homo sapiens ?
-
 ?
3.4.24.B15 o-aminobenzoyl-A110WLDSGVQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O sequence is based on residues 107-139 sequence of the enzyme, cleavage site is the Leu-Asp bond Homo sapiens o-aminobenzoyl-A110WL + DSGVQ-N-(2,4-dinitrophenyl)ethylene diamine
-
 ?
3.4.24.B15 o-aminobenzoyl-D124HLSDTSTQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O sequence is based on residues 107-139 sequence of the enzyme, cleavage site is the Ser-Asp bond Homo sapiens o-aminobenzoyl-D124HLS + DTSTQ-N-(2,4-dinitrophenyl)ethylene diamine
-
 ?
3.4.24.B15 o-aminobenzoyl-G197QRDSQAQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O sequence is based on the MEPE protein sequence Homo sapiens o-aminobenzoyl-G197QR + DSQAQ-N-(2,4-dinitrophenyl)ethylene diamine
-
 ?
3.4.24.B15 o-aminobenzoyl-G337SNDIMGQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O sequence is based on the MEPE protein sequence Homo sapiens o-aminobenzoyl-G337SN + DIMGQ-N-(2,4-dinitrophenyl)ethylene diamine
-
 ?
3.4.24.B15 o-aminobenzoyl-G386SSDAAEQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O sequence is based on the MEPE protein sequence Homo sapiens o-aminobenzoyl-G386SS + DAAEQ-N-(2,4-dinitrophenyl)ethylene diamine
-
 ?
3.4.24.B15 o-aminobenzoyl-GFSDYK-(2,4-dinitrophenyl)-NH2 + H2O cleavage site is the Ser-Asp bond Homo sapiens o-aminobenzoyl-GFS + DYK-(2,4-dinitrophenyl)-NH2
-
 ?
3.4.24.B15 o-aminobenzoyl-GFSDYK-(2,4-dinitrophenyl)-OH + H2O best substrate, cleavage site is the Ser-Asp bond Homo sapiens o-aminobenzoyl-GFS + DYK-(2,4-dinitrophenyl)-OH
-
 ?
3.4.24.B15 o-aminobenzoyl-GFSDYQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O cleavage site is the Ser-Asp bond Homo sapiens o-aminobenzoyl-GFS + DYQ-N-(2,4-dinitrophenyl)ethylene diamine
-
 ?
3.4.24.B15 o-aminobenzoyl-GFSEY-(2,4-dinitrophenyl)K + H2O low activity, cleavage site is the Ser-Asp bond Homo sapiens o-aminobenzoyl-GFS + EY-(2,4-dinitrophenyl)K
-
 ?
3.4.24.B15 o-aminobenzoyl-IPSDFEGQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O sequence is based on the MEPE protein sequence Homo sapiens o-aminobenzoyl-IPS + DFEGQ-N-(2,4-dinitrophenyl)ethylene diamine
-
 ?
3.4.24.B15 o-aminobenzoyl-L134ELDSRQ-(2,4-dinitrophenyl)ethylene diamine + H2O sequence is based on residues 107-139 sequence of the enzyme, cleavage site is the Leu-Asp bond Homo sapiens o-aminobenzoyl-L134EL + DSRQ-N-(2,4-dinitrophenyl)ethylene diamine
-
 ?
3.4.24.B15 o-aminobenzoyl-L94MMDFRGQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O sequence is based on the FGF-23 protein sequence, cleavage site is the Met-Asp bond Homo sapiens o-aminobenzoyl-L94MM + DFRGQ-N-(2,4-dinitrophenyl)ethylene diamine
-
 ?
3.4.24.B15 o-aminobenzoyl-N122GYDVYHQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O sequence is based on the FGF-23 protein sequence, cleavage site is the Tyr-Asp bond Homo sapiens o-aminobenzoyl-N122GY + DVYHQ-N-(2,4-dinitrophenyl)ethylene diamine
-
 ?
3.4.24.B15 o-aminobenzoyl-N449EMDSFNQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O sequence is based on the MEPE protein sequence Homo sapiens o-aminobenzoyl-N449EM + DSFNQ-N-(2,4-dinitrophenyl)ethylene diamine
-
 ?
3.4.24.B15 o-aminobenzoyl-R175RHTQSAEDDSERQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O sequence is based on the FGF-23 protein sequence, cleavage site is the Asp-Asp bond Homo sapiens o-aminobenzoyl-R175RHTQSAED + DSERQ-N-(2,4-dinitrophenyl)ethylene diamine
-
 ?
3.4.24.B15 o-aminobenzoyl-R175RHTRSAEDDSERQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O sequence is based on the FGF-23 protein sequence, cleavage site is the Asp-Asp bond Homo sapiens o-aminobenzoyl-R175RHTRSAED + DSERQ-N-(2,4-dinitrophenyl)ethylene diamine
-
 ?
3.4.24.B15 o-aminobenzoyl-R441GLDNEIQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O sequence is based on the MEPE protein sequence Homo sapiens o-aminobenzoyl-R441GL + DNEIQ-N-(2,4-dinitrophenyl)ethylene diamine
-
 ?
3.4.24.B15 o-aminobenzoyl-R506RDDSSEQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O sequence is based on the MEPE protein sequence Homo sapiens o-aminobenzoyl-R506RD + DSSEQ-N-(2,4-dinitrophenyl)ethylene diamine
-
 ?
3.4.24.B15 o-aminobenzoyl-R76SEDAGFQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O sequence is based on the FGF-23 protein sequence, cleavage site is the Glu-Asp bond Homo sapiens o-aminobenzoyl-R76SE + DAGFQ-N-(2,4-dinitrophenyl)ethylene diamine
-
 ?
3.4.24.B15 o-aminobenzoyl-S212AEDNSPQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O sequence is based on the FGF-23 protein sequence, cleavage site is the Glu-Asp bond Homo sapiens o-aminobenzoyl-S212AE + DNSPQ-N-(2,4-dinitrophenyl)ethylene diamine
-
 ?
3.4.24.B15 o-aminobenzoyl-S513SDSGSQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O sequence is based on the MEPE protein sequence Homo sapiens o-aminobenzoyl-S513S + DSGSQ-N-(2,4-dinitrophenyl)ethylene diamine
-
 ?
3.4.24.B15 o-aminobenzoyl-T294HLDTKKQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O sequence is based on the MEPE protein sequence Homo sapiens o-aminobenzoyl-T294HL + DTKKQ-N-(2,4-dinitrophenyl)ethylene diamine
-
 ?
3.4.24.B15 protein + H2O
-
 Homo sapiens peptides
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.B15 M13.091 Merops-ID Homo sapiens
3.4.24.B15 PHEX encoded by PHEx i.e. phosphate-regulating gene with homologies to endopeptidase on the X-chromosome Homo sapiens
3.4.24.B15 PHEX
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.24.B15 0.01
-
 o-aminobenzoyl-N122GYDVYHQ-N-(2,4-dinitrophenyl)ethylene diamine recombinant soluble enzyme, pH 5.5, 37°C Homo sapiens
3.4.24.B15 0.01
-
 o-aminobenzoyl-G337SNDIMGQ-N-(2,4-dinitrophenyl)ethylene diamine recombinant soluble enzyme, pH 5.5, 37°C Homo sapiens
3.4.24.B15 0.02
-
 o-aminobenzoyl-T294HLDTKKQ-N-(2,4-dinitrophenyl)ethylene diamine recombinant soluble enzyme, pH 5.5, 37°C Homo sapiens
3.4.24.B15 0.03
-
 o-aminobenzoyl-R441GLDNEIQ-N-(2,4-dinitrophenyl)ethylene diamine recombinant soluble enzyme, pH 5.5, 37°C Homo sapiens
3.4.24.B15 0.04
-
 o-aminobenzoyl-A110WLDSGVQ-N-(2,4-dinitrophenyl)ethylene diamine recombinant soluble enzyme, pH 5.5, 37°C Homo sapiens
3.4.24.B15 0.05
-
 o-aminobenzoyl-L134ELDSRQ-(2,4-dinitrophenyl)ethylene diamine recombinant soluble enzyme, pH 5.5, 37°C Homo sapiens
3.4.24.B15 0.06
-
 o-aminobenzoyl-R76SEDAGFQ-N-(2,4-dinitrophenyl)ethylene diamine recombinant soluble enzyme, pH 5.5, 37°C Homo sapiens
3.4.24.B15 0.1
-
 o-aminobenzoyl-D124HLSDTSTQ-N-(2,4-dinitrophenyl)ethylene diamine recombinant soluble enzyme, pH 5.5, 37°C Homo sapiens
3.4.24.B15 0.15
-
 o-aminobenzoyl-R175RHTQSAEDDSERQ-N-(2,4-dinitrophenyl)ethylene diamine recombinant soluble enzyme, pH 5.5, 37°C Homo sapiens
3.4.24.B15 0.2
-
 o-aminobenzoyl-R175RHTRSAEDDSERQ-N-(2,4-dinitrophenyl)ethylene diamine recombinant soluble enzyme, pH 5.5, 37°C Homo sapiens
3.4.24.B15 0.2
-
 o-aminobenzoyl-S513SDSGSQ-N-(2,4-dinitrophenyl)ethylene diamine recombinant soluble enzyme, pH 5.5, 37°C Homo sapiens
3.4.24.B15 0.2
-
 o-aminobenzoyl-G197QRDSQAQ-N-(2,4-dinitrophenyl)ethylene diamine recombinant soluble enzyme, pH 5.5, 37°C Homo sapiens
3.4.24.B15 0.3
-
 o-aminobenzoyl-G386SSDAAEQ-N-(2,4-dinitrophenyl)ethylene diamine recombinant soluble enzyme, pH 5.5, 37°C Homo sapiens
3.4.24.B15 0.3
-
 o-aminobenzoyl-S212AEDNSPQ-N-(2,4-dinitrophenyl)ethylene diamine recombinant soluble enzyme, pH 5.5, 37°C Homo sapiens
3.4.24.B15 0.3
-
 o-aminobenzoyl-N449EMDSFNQ-N-(2,4-dinitrophenyl)ethylene diamine recombinant soluble enzyme, pH 5.5, 37°C Homo sapiens
3.4.24.B15 0.5
-
 o-aminobenzoyl-GFSDYK-(2,4-dinitrophenyl)-OH recombinant soluble enzyme, pH 5.5, 37°C Homo sapiens
3.4.24.B15 0.5
-
 o-aminobenzoyl-R506RDDSSEQ-N-(2,4-dinitrophenyl)ethylene diamine recombinant soluble enzyme, pH 5.5, 37°C Homo sapiens
3.4.24.B15 0.6
-
 o-aminobenzoyl-GFSDYQ-N-(2,4-dinitrophenyl)ethylene diamine recombinant soluble enzyme, pH 5.5, 37°C Homo sapiens
3.4.24.B15 0.8
-
 o-aminobenzoyl-L94MMDFRGQ-N-(2,4-dinitrophenyl)ethylene diamine recombinant soluble enzyme, pH 5.5, 37°C Homo sapiens
3.4.24.B15 1.3
-
 o-aminobenzoyl-GFSDYK-(2,4-dinitrophenyl)-NH2 recombinant soluble enzyme, pH 5.5, 37°C Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.B15 5.5
-
-
 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.24.B15 3.5 8.5
-
 Homo sapiens