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Literature summary extracted from

  • Raux, E.; Leech, H.K.; Beck, R.; Schubert, H.L.; Santander, P.J.; Roessner, C.A.; Scott, A.I.; Martens, J.H.; Jahn, D.; Thermes, C.; Rambach, A.; Warren, M.J.
    Identification and functional analysis of enzymes required for precorrin-2 dehydrogenation and metal ion insertion in the biosynthesis of sirohaem and cobalamin in Bacillus megaterium (2003), Biochem. J., 370, 505-516.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.76 sequenced and overproduced, sirABC gene cluster complements Escherichia coli cysG mutants Priestia megaterium
4.99.1.4 sirB, expression in Escherichia coli BL21(DE3)pLysS, sirA,B,C operon, amino acid sequence Priestia megaterium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.99.1.4 soluble recombinant SirB Priestia megaterium
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.76 22000
-
 2 * 22000, homodimer, SDS-PAGE Priestia megaterium
1.3.1.76 45000
-
 gel filtration Priestia megaterium
4.99.1.4 33000
-
 1 * 33000, SDS-PAGE Priestia megaterium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.76 precorrin-2 + NAD+ Priestia megaterium siroheme and cobalamin biosynthesis, second of three steps leading to formation of siroheme from uroporphyrinogen III sirohydrochlorin + NADH + H+
-
 ?
1.3.1.76 precorrin-2 + NAD+ Priestia megaterium DSM 509 siroheme and cobalamin biosynthesis, second of three steps leading to formation of siroheme from uroporphyrinogen III sirohydrochlorin + NADH + H+
-
 ?
4.99.1.4 sirohydrochlorin + Fe2+ Priestia megaterium SirB is responsible for the final step in siroheme synthesis siroheme + 2 H+
-
 ?
4.99.1.4 sirohydrochlorin + Fe2+ Priestia megaterium DSM 509 SirB is responsible for the final step in siroheme synthesis siroheme + 2 H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.76 Priestia megaterium P61818 DSM509
-
1.3.1.76 Priestia megaterium DSM 509 P61818 DSM509
-
4.99.1.4 Priestia megaterium
-
 strain DSM509
-
4.99.1.4 Priestia megaterium DSM 509
-
 strain DSM509
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.76
-
 Priestia megaterium
4.99.1.4 recombinant SirB Priestia megaterium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.76 0.06
-
-
 Priestia megaterium
4.99.1.4 0.0034
-
 pH 8, cobaltochelation of precorrin-2 Priestia megaterium
4.99.1.4 0.337
-
 pH 8, cobaltochelation of sirohydrochlorin Priestia megaterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.76 precorrin-2 + NAD+ unlike Met8p and CysG, SirC has no chelatase activity Priestia megaterium sirohydrochlorin + NADH + H+
-
 ?
1.3.1.76 precorrin-2 + NAD+ siroheme and cobalamin biosynthesis, second of three steps leading to formation of siroheme from uroporphyrinogen III Priestia megaterium sirohydrochlorin + NADH + H+
-
 ?
1.3.1.76 precorrin-2 + NAD+ unlike Met8p and CysG, SirC has no chelatase activity Priestia megaterium DSM 509 sirohydrochlorin + NADH + H+
-
 ?
1.3.1.76 precorrin-2 + NAD+ siroheme and cobalamin biosynthesis, second of three steps leading to formation of siroheme from uroporphyrinogen III Priestia megaterium DSM 509 sirohydrochlorin + NADH + H+
-
 ?
4.99.1.4 precorrin-2 + Co2+ SirB, much lower specific activity than with sirohydrochlorin Priestia megaterium cobalt-precorrin-2 + 2 H+
-
 ?
4.99.1.4 precorrin-2 + Co2+ SirB, much lower specific activity than with sirohydrochlorin Priestia megaterium DSM 509 cobalt-precorrin-2 + 2 H+
-
 ?
4.99.1.4 sirohydrochlorin + Co2+ SirB, lower specificity for cobalt than for iron Priestia megaterium cobalt-sirohydrochlorin + 2 H+
-
 ?
4.99.1.4 sirohydrochlorin + Co2+ SirB, lower specificity for cobalt than for iron Priestia megaterium DSM 509 cobalt-sirohydrochlorin + 2 H+
-
 ?
4.99.1.4 sirohydrochlorin + Fe2+ SirB, monofunctional ferrochelatase, higher specificity for iron over cobalt Priestia megaterium siroheme + 2 H+
-
 ?
4.99.1.4 sirohydrochlorin + Fe2+ SirB is responsible for the final step in siroheme synthesis Priestia megaterium siroheme + 2 H+
-
 ?
4.99.1.4 sirohydrochlorin + Fe2+ SirB, monofunctional ferrochelatase, higher specificity for iron over cobalt Priestia megaterium DSM 509 siroheme + 2 H+
-
 ?
4.99.1.4 sirohydrochlorin + Fe2+ SirB is responsible for the final step in siroheme synthesis Priestia megaterium DSM 509 siroheme + 2 H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.76 dimer 2 * 22000, homodimer, SDS-PAGE Priestia megaterium
4.99.1.4 monomer 1 * 33000, SDS-PAGE Priestia megaterium

Synonyms

EC Number Synonyms Comment Organism
1.3.1.76 precorrin-2 dehydrogenase
-
 Priestia megaterium
1.3.1.76 SirC
-
 Priestia megaterium
4.99.1.4 SirB Bacillus megaterium Priestia megaterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.99.1.4 8
-
 assay at Priestia megaterium