BRENDA - Enzyme Database show

Mammalian class Sigma glutathione S-transferases: catalytic properties and tissue-specific expression of human and rat GSH-dependent prostaglandin D2 synthases

Jowsey, I.R.; Thomson, A.M.; Flanagan, J.U.; Murdock, P.R.; Moore, G.B.T.; Meyer, D.J.; Murphy, G.J.; Smith, S.A.; Hayes, J.D.; Biochem. J. 359, 507-516 (2001)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
5.3.99.2
expression of cDNA in Escherichia coli
Homo sapiens
5.3.99.2
expression of cDNA in Escherichia coli
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.3.99.2
0.2
-
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
-
Homo sapiens
5.3.99.2
0.2
-
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
-
Rattus norvegicus
5.3.99.2
0.2
-
glutathione
-
Homo sapiens
5.3.99.2
0.3
-
glutathione
; glutathione S-transferase activity
Rattus norvegicus
5.3.99.2
3.2
-
1-chloro-2,4-dinitrobenzene
-
Homo sapiens
5.3.99.2
5
-
1-chloro-2,4-dinitrobenzene
-
Rattus norvegicus
5.3.99.2
8
-
glutathione
glutathione S-transferase activity
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.3.99.2
23000
-
x * 23000, SDS-PAGE
Homo sapiens
5.3.99.2
23000
-
x * 23000, SDS-PAGE
Rattus norvegicus
5.3.99.2
23298
-
x * 23298, deduced from nucleotide sequence
Rattus norvegicus
5.3.99.2
23359
-
x * 23359, deduced from nucleotide sequence
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.3.99.2
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
Homo sapiens
-
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate
-
Homo sapiens
?
5.3.99.2
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
Rattus norvegicus
-
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate
-
Rattus norvegicus
?
5.3.99.2
1-bromo-2,4-dinitrobenzene + glutathione
Homo sapiens
-
?
-
Homo sapiens
?
5.3.99.2
1-bromo-2,4-dinitrobenzene + glutathione
Rattus norvegicus
-
?
-
Rattus norvegicus
?
5.3.99.2
1-chloro-2,4-dinitrobenzene + glutathione
Homo sapiens
-
?
-
Homo sapiens
?
5.3.99.2
1-chloro-2,4-dinitrobenzene + glutathione
Rattus norvegicus
-
?
-
Rattus norvegicus
?
5.3.99.2
1-fluoro-2,4-dinitrobenzene + glutathione
Homo sapiens
-
?
-
Homo sapiens
?
5.3.99.2
1-fluoro-2,4-dinitrobenzene + glutathione
Rattus norvegicus
-
?
-
Rattus norvegicus
?
5.3.99.2
1-iodo-2,4-dinitrobenzene + glutathione
Homo sapiens
-
?
-
Homo sapiens
?
5.3.99.2
1-iodo-2,4-dinitrobenzene + glutathione
Rattus norvegicus
-
?
-
Rattus norvegicus
?
5.3.99.2
allyl isothiocyanate + glutathione
Homo sapiens
-
?
-
Homo sapiens
?
5.3.99.2
allyl isothiocyanate + glutathione
Rattus norvegicus
-
?
-
Rattus norvegicus
?
5.3.99.2
benzyl isothiocyanate + glutathione
Homo sapiens
-
?
-
Homo sapiens
?
5.3.99.2
benzyl isothiocyanate + glutathione
Rattus norvegicus
-
?
-
Rattus norvegicus
?
5.3.99.2
cumene hydroperoxide + 2 GSH
Homo sapiens
-
cumene hydroxide + GSSG + H2O
-
Homo sapiens
?
5.3.99.2
cumene hydroperoxide + 2 GSH
Rattus norvegicus
-
cumene hydroxide + GSSG + H2O
-
Rattus norvegicus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.3.99.2
Homo sapiens
-
-
-
5.3.99.2
Rattus norvegicus
O35543
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.3.99.2
recombinant PGDS
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.2
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
-
649720
Homo sapiens
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate
-
649720
Homo sapiens
?
5.3.99.2
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
-
649720
Rattus norvegicus
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate
-
649720
Rattus norvegicus
?
5.3.99.2
1-bromo-2,4-dinitrobenzene + glutathione
-
649720
Homo sapiens
?
-
649720
Homo sapiens
?
5.3.99.2
1-bromo-2,4-dinitrobenzene + glutathione
-
649720
Rattus norvegicus
?
-
649720
Rattus norvegicus
?
5.3.99.2
1-chloro-2,4-dinitrobenzene + glutathione
-
649720
Homo sapiens
?
-
649720
Homo sapiens
?
5.3.99.2
1-chloro-2,4-dinitrobenzene + glutathione
-
649720
Rattus norvegicus
?
-
649720
Rattus norvegicus
?
5.3.99.2
1-fluoro-2,4-dinitrobenzene + glutathione
-
649720
Homo sapiens
?
-
649720
Homo sapiens
?
5.3.99.2
1-fluoro-2,4-dinitrobenzene + glutathione
-
649720
Rattus norvegicus
?
-
649720
Rattus norvegicus
?
5.3.99.2
1-iodo-2,4-dinitrobenzene + glutathione
-
649720
Homo sapiens
?
-
649720
Homo sapiens
?
5.3.99.2
1-iodo-2,4-dinitrobenzene + glutathione
-
649720
Rattus norvegicus
?
-
649720
Rattus norvegicus
?
5.3.99.2
allyl isothiocyanate + glutathione
-
649720
Homo sapiens
?
-
649720
Homo sapiens
?
5.3.99.2
allyl isothiocyanate + glutathione
-
649720
Rattus norvegicus
?
-
649720
Rattus norvegicus
?
5.3.99.2
benzyl isothiocyanate + glutathione
-
649720
Homo sapiens
?
-
649720
Homo sapiens
?
5.3.99.2
benzyl isothiocyanate + glutathione
-
649720
Rattus norvegicus
?
-
649720
Rattus norvegicus
?
5.3.99.2
cumene hydroperoxide + 2 GSH
-
649720
Homo sapiens
cumene hydroxide + GSSG + H2O
-
649720
Homo sapiens
?
5.3.99.2
cumene hydroperoxide + 2 GSH
-
649720
Rattus norvegicus
cumene hydroxide + GSSG + H2O
-
649720
Rattus norvegicus
?
Subunits
EC Number
Subunits
Commentary
Organism
5.3.99.2
?
x * 23000, SDS-PAGE; x * 23359, deduced from nucleotide sequence
Homo sapiens
5.3.99.2
?
x * 23000, SDS-PAGE; x * 23298, deduced from nucleotide sequence
Rattus norvegicus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
5.3.99.2
glutathione
-
Rattus norvegicus
5.3.99.2
glutathione
-
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.2
expression of cDNA in Escherichia coli
Homo sapiens
5.3.99.2
expression of cDNA in Escherichia coli
Rattus norvegicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
5.3.99.2
glutathione
-
Rattus norvegicus
5.3.99.2
glutathione
-
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.3.99.2
0.2
-
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
-
Homo sapiens
5.3.99.2
0.2
-
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
-
Rattus norvegicus
5.3.99.2
0.2
-
glutathione
-
Homo sapiens
5.3.99.2
0.3
-
glutathione
; glutathione S-transferase activity
Rattus norvegicus
5.3.99.2
3.2
-
1-chloro-2,4-dinitrobenzene
-
Homo sapiens
5.3.99.2
5
-
1-chloro-2,4-dinitrobenzene
-
Rattus norvegicus
5.3.99.2
8
-
glutathione
glutathione S-transferase activity
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.3.99.2
23000
-
x * 23000, SDS-PAGE
Homo sapiens
5.3.99.2
23000
-
x * 23000, SDS-PAGE
Rattus norvegicus
5.3.99.2
23298
-
x * 23298, deduced from nucleotide sequence
Rattus norvegicus
5.3.99.2
23359
-
x * 23359, deduced from nucleotide sequence
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.3.99.2
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
Homo sapiens
-
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate
-
Homo sapiens
?
5.3.99.2
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
Rattus norvegicus
-
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate
-
Rattus norvegicus
?
5.3.99.2
1-bromo-2,4-dinitrobenzene + glutathione
Homo sapiens
-
?
-
Homo sapiens
?
5.3.99.2
1-bromo-2,4-dinitrobenzene + glutathione
Rattus norvegicus
-
?
-
Rattus norvegicus
?
5.3.99.2
1-chloro-2,4-dinitrobenzene + glutathione
Homo sapiens
-
?
-
Homo sapiens
?
5.3.99.2
1-chloro-2,4-dinitrobenzene + glutathione
Rattus norvegicus
-
?
-
Rattus norvegicus
?
5.3.99.2
1-fluoro-2,4-dinitrobenzene + glutathione
Homo sapiens
-
?
-
Homo sapiens
?
5.3.99.2
1-fluoro-2,4-dinitrobenzene + glutathione
Rattus norvegicus
-
?
-
Rattus norvegicus
?
5.3.99.2
1-iodo-2,4-dinitrobenzene + glutathione
Homo sapiens
-
?
-
Homo sapiens
?
5.3.99.2
1-iodo-2,4-dinitrobenzene + glutathione
Rattus norvegicus
-
?
-
Rattus norvegicus
?
5.3.99.2
allyl isothiocyanate + glutathione
Homo sapiens
-
?
-
Homo sapiens
?
5.3.99.2
allyl isothiocyanate + glutathione
Rattus norvegicus
-
?
-
Rattus norvegicus
?
5.3.99.2
benzyl isothiocyanate + glutathione
Homo sapiens
-
?
-
Homo sapiens
?
5.3.99.2
benzyl isothiocyanate + glutathione
Rattus norvegicus
-
?
-
Rattus norvegicus
?
5.3.99.2
cumene hydroperoxide + 2 GSH
Homo sapiens
-
cumene hydroxide + GSSG + H2O
-
Homo sapiens
?
5.3.99.2
cumene hydroperoxide + 2 GSH
Rattus norvegicus
-
cumene hydroxide + GSSG + H2O
-
Rattus norvegicus
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.2
recombinant PGDS
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.2
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
-
649720
Homo sapiens
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate
-
649720
Homo sapiens
?
5.3.99.2
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
-
649720
Rattus norvegicus
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate
-
649720
Rattus norvegicus
?
5.3.99.2
1-bromo-2,4-dinitrobenzene + glutathione
-
649720
Homo sapiens
?
-
649720
Homo sapiens
?
5.3.99.2
1-bromo-2,4-dinitrobenzene + glutathione
-
649720
Rattus norvegicus
?
-
649720
Rattus norvegicus
?
5.3.99.2
1-chloro-2,4-dinitrobenzene + glutathione
-
649720
Homo sapiens
?
-
649720
Homo sapiens
?
5.3.99.2
1-chloro-2,4-dinitrobenzene + glutathione
-
649720
Rattus norvegicus
?
-
649720
Rattus norvegicus
?
5.3.99.2
1-fluoro-2,4-dinitrobenzene + glutathione
-
649720
Homo sapiens
?
-
649720
Homo sapiens
?
5.3.99.2
1-fluoro-2,4-dinitrobenzene + glutathione
-
649720
Rattus norvegicus
?
-
649720
Rattus norvegicus
?
5.3.99.2
1-iodo-2,4-dinitrobenzene + glutathione
-
649720
Homo sapiens
?
-
649720
Homo sapiens
?
5.3.99.2
1-iodo-2,4-dinitrobenzene + glutathione
-
649720
Rattus norvegicus
?
-
649720
Rattus norvegicus
?
5.3.99.2
allyl isothiocyanate + glutathione
-
649720
Homo sapiens
?
-
649720
Homo sapiens
?
5.3.99.2
allyl isothiocyanate + glutathione
-
649720
Rattus norvegicus
?
-
649720
Rattus norvegicus
?
5.3.99.2
benzyl isothiocyanate + glutathione
-
649720
Homo sapiens
?
-
649720
Homo sapiens
?
5.3.99.2
benzyl isothiocyanate + glutathione
-
649720
Rattus norvegicus
?
-
649720
Rattus norvegicus
?
5.3.99.2
cumene hydroperoxide + 2 GSH
-
649720
Homo sapiens
cumene hydroxide + GSSG + H2O
-
649720
Homo sapiens
?
5.3.99.2
cumene hydroperoxide + 2 GSH
-
649720
Rattus norvegicus
cumene hydroxide + GSSG + H2O
-
649720
Rattus norvegicus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
5.3.99.2
?
x * 23000, SDS-PAGE; x * 23359, deduced from nucleotide sequence
Homo sapiens
5.3.99.2
?
x * 23000, SDS-PAGE; x * 23298, deduced from nucleotide sequence
Rattus norvegicus