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Literature summary extracted from

  • Wainszelbaum, M.; Isola, E.; Wilkowsky, S.; Cannata, J.J.B.; Florin-Christensen, J.; Florin-Christensen, M.
    Lysosomal phospholipase A1 in Trypanosoma cruzi: an enzyme with a possible role in the pathogenesis of Chagas' disease (2001), Biochem. J., 355, 765-770.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.32 Triton X-100 1%, 12.4fold increase in activity Trypanosoma cruzi

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.32 chlorpromazine 1 mM, 50% inhibition Trypanosoma cruzi

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.32 lysosome
-
Trypanosoma cruzi 5764
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.32 38000
-
1 * 38000, SDS-PAGE Trypanosoma cruzi
3.1.1.32 40000
-
gel filtration Trypanosoma cruzi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.32 1-palmitoyl-2-oleoyl-phosphatidylcholine + H2O Trypanosoma cruzi
-
2-oleoyl-lysophosphatidylcholine + palmitic acid
-
?
3.1.1.32 phosphatidylcholine + H2O Trypanosoma cruzi
-
2-acylglycerophosphocholine + a carboxylate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.32 Trypanosoma cruzi
-
Plase A1 may play a role in the pathogenesis of Chagas' disease
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.32 glycoprotein indicated by binding to ConA-Sepharose Trypanosoma cruzi

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.32 purified from epimastogote homogenate supernatants, DEAE-Trisacryl, ConA-Sepharose 4B, CM-Sepharose Trypanosoma cruzi

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.32 0.35
-
-
Trypanosoma cruzi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.32 1-palmitoyl-2-oleoyl-phosphatidylcholine + H2O
-
Trypanosoma cruzi 2-oleoyl-lysophosphatidylcholine + palmitic acid
-
?
3.1.1.32 phosphatidylcholine + H2O
-
Trypanosoma cruzi 2-acylglycerophosphocholine + a carboxylate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.1.32 monomer 1 * 38000, SDS-PAGE Trypanosoma cruzi

Synonyms

EC Number Synonyms Comment Organism
3.1.1.32 Plase A1
-
Trypanosoma cruzi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.32 4 5
-
Trypanosoma cruzi

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.32 3 7 approx. 50% of maximal activity at pH 3.0, approx. 20% of maximal activity at pH 6.0 Trypanosoma cruzi