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Literature summary extracted from

  • Okoniewska, M.; Tanaka, T.; Yada, R.Y.
    The pepsin residue glycine-76 contributes to active-site loop flexibility and participates in catalysis (2000), Biochem. J., 349, 169-177.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.23.1 expression in Escherichia coli CJ236 Sus scrofa

Protein Variants

EC Number Protein Variants Comment Organism
3.4.23.1 G76A mutant enzyme with lower catalytic efficiency and is activated more slowly than the wild-type enzyme. The slower activation process is associated directly with altered proteolytic activity Sus scrofa
3.4.23.1 G76S mutant enzyme with lower catalytic efficiency and is activated more slowly than the wild-type enzyme. The slower activation process is associated directly with altered proteolytic activity Sus scrofa
3.4.23.1 G76V mutant enzyme with lower catalytic efficiency and is activated more slowly than the wild-type enzyme. The slower activation process is associated directly with altered proteolytic activity Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.23.1 0.011
-
 Leu-Ser-Phe(p-NO2)-Nle-Ala-Leu pH 3.95, 37°C, wild-type enzyme Sus scrofa
3.4.23.1 0.04
-
 Leu-Ser-Phe(p-NO2)-Nle-Ala-Leu pH 3.95, 37°C, mutant enzyme G76A Sus scrofa
3.4.23.1 0.041
-
 Leu-Ser-Phe(p-NO2)-Nle-Ala-Leu pH 3.95, 37°C, mutant enzyme G76S Sus scrofa
3.4.23.1 0.043
-
 Lys-Pro-Ala-Glu-Phe-Phe(p-NO2)-Ala-Leu pH 2.1, 37°C, wild-type enzyme Sus scrofa
3.4.23.1 0.044
-
 Leu-Ser-Phe(p-NO2)-Nle-Ala-Leu pH 3.95, 37°C, mutant enzyme G76V Sus scrofa
3.4.23.1 0.071
-
 Lys-Pro-Ala-Glu-Phe-Phe(p-NO2)-Ala-Leu pH 2.1, 37°C, mutant enzyme G76V Sus scrofa
3.4.23.1 0.085
-
 Lys-Pro-Ala-Glu-Phe-Phe(p-NO2)-Ala-Leu pH 2.1, 37°C, mutant enzyme G76S Sus scrofa
3.4.23.1 0.098
-
 Lys-Pro-Ala-Glu-Phe-Phe(p-NO2)-Ala-Leu pH 2.1, 37°C, mutant enzyme G76A Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
3.4.23.1 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.23.1
-
 Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.23.1 Leu-Ser-Phe(p-NO2)-Nle-Ala-Leu + H2O
-
 Sus scrofa ?
-
 ?
3.4.23.1 Lys-Pro-Ala-Glu-Phe-Phe(p-NO2)-Ala-Leu + H2O
-
 Sus scrofa ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.23.1 1.2
-
 Leu-Ser-Phe(p-NO2)-Nle-Ala-Leu pH 3.95, 37°C, mutant enzyme G76V Sus scrofa
3.4.23.1 2.5
-
 Lys-Pro-Ala-Glu-Phe-Phe(p-NO2)-Ala-Leu pH 2.1, 37°C, mutant enzyme G76S Sus scrofa
3.4.23.1 2.9
-
 Leu-Ser-Phe(p-NO2)-Nle-Ala-Leu pH 3.95, 37°C, mutant enzyme G76S Sus scrofa
3.4.23.1 11.1
-
 Lys-Pro-Ala-Glu-Phe-Phe(p-NO2)-Ala-Leu pH 2.1, 37°C, mutant enzyme G76V Sus scrofa
3.4.23.1 23.8
-
 Leu-Ser-Phe(p-NO2)-Nle-Ala-Leu pH 3.95, 37°C, wild-type enzyme Sus scrofa
3.4.23.1 43.4
-
 Leu-Ser-Phe(p-NO2)-Nle-Ala-Leu pH 3.95, 37°C, mutant enzyme G76A Sus scrofa
3.4.23.1 47
-
 Lys-Pro-Ala-Glu-Phe-Phe(p-NO2)-Ala-Leu pH 2.1, 37°C, mutant enzyme G76A Sus scrofa
3.4.23.1 88.7
-
 Leu-Ser-Phe(p-NO2)-Nle-Ala-Leu pH 3.95, 37°C, wild-type enzyme Sus scrofa
3.4.23.1 180.5
-
 Lys-Pro-Ala-Glu-Phe-Phe(p-NO2)-Ala-Leu pH 2.1, 37°C, wild-type enzyme Sus scrofa
3.4.23.1 181
-
 Lys-Pro-Ala-Glu-Phe-Phe(p-NO2)-Ala-Leu pH 2.1, 37°C, wild-type enzyme Sus scrofa