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Literature summary extracted from

  • Basak, A.; Toure, B.B.; Lazure, C.; Mbikay, M.; Chretien, M.; Seidah, N.G.
    Enzymic characterization in vitro of recombinant proprotein convertase PC4 (1999), Biochem. J., 343, 29-37.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.21.B24 dithiothreitol 2.5fold activation at 1 mM Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.B24 4-chloromercuribenzoate strong inhibition Rattus norvegicus
3.4.21.B24 CDTA complete inhibition at 1-10 mM Rattus norvegicus
3.4.21.B24 Co2+ order of decreasing inhibitory effect: Cu2+, Hg2+, Zn2+, Ni2+, Co2+, 50% inhibition at 0.25 mM Rattus norvegicus
3.4.21.B24 Cu2+ order of decreasing inhibitory effect: Cu2+, Hg2+, Zn2+, Ni2+, Co2+, 50% inhibition at 0.012 mM Rattus norvegicus
3.4.21.B24 EDTA complete inhibition at 1-10 mM Rattus norvegicus
3.4.21.B24 EGTA complete inhibition at 1-10 mM Rattus norvegicus
3.4.21.B24 Hg2+ order of decreasing inhibitory effect: Cu2+, Hg2+, Zn2+, Ni2+, Co2+ Rattus norvegicus
3.4.21.B24 Ni2+ order of decreasing inhibitory effect: Cu2+, Hg2+, Zn2+, Ni2+, Co2+ Rattus norvegicus
3.4.21.B24 Zn2+ order of decreasing inhibitory effect: Cu2+, Hg2+, Zn2+, Ni2+, Co2+ Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.B24 0.15
-
 acetyl-Arg-Lys-Lys-Arg-4-methylcoumarin-7-amide pH 7 Rattus norvegicus
3.4.21.B24 6.6
-
 acetyl-Arg-Ser-Lys-Arg-4-methylcoumarin-7-amide pH 7 Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.B24 Ca2+ dependent, optimal concentration: 2 mM Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.B24 54000
-
 SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.B24 pro-insulin growth factor + H2O Rattus norvegicus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.B24 Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.B24 acetyl-Arg-Lys-Lys-Arg-4-methylcoumarin-7-amide + H2O preferred substrate Rattus norvegicus ?
-
 ?
3.4.21.B24 acetyl-Arg-Ser-Lys-Arg-4-methylcoumarin-7-amide + H2O
-
 Rattus norvegicus ?
-
 ?
3.4.21.B24 acetyl-PAKSARSVRA + H2O sequence derived from pro-insulin growth factor Rattus norvegicus acetyl-PAKSAR + SVRA
-
 ?
3.4.21.B24 acetyl-PAKSERDVST + H2O sequence derived from pro-insulin growth factor Rattus norvegicus acetyl-PAKSER + DVST
-
 ?
3.4.21.B24 pro-insulin growth factor + H2O
-
 Rattus norvegicus ?
-
 ?
3.4.21.B24 REKR-4-methylcoumarin-7-amide + H2O
-
 Rattus norvegicus ?
-
 ?
3.4.21.B24 RSKR-4-methylcoumarin-7-amide + H2O
-
 Rattus norvegicus RSKR + 7-amino-4-methylcoumarin
-
 ?
3.4.21.B24 RVRR-4-methylcoumarin-7-amide + H2O
-
 Rattus norvegicus ?
-
 ?
3.4.21.B24 YQTLRRRVKRSLVVPTD + H2O preferred substrate Rattus norvegicus YQTLRRRVKR + SLVVPTD
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.B24 PC4A
-
 Rattus norvegicus
3.4.21.B24 proprotein convertase PC4
-
 Rattus norvegicus
3.4.21.B24 S08.074 Merops ID Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.B24 7
-
-
 Rattus norvegicus