BRENDA - Enzyme Database show

Sequence, catalytic properties and expression of chicken glutathione-dependent prostaglandin D2 synthase, a novel class sigma glutathione S-transferase

Thomson, A.M.; Meyer, D.J.; Hayes, J.D.; Biochem. J. 333, 317-325 (1998)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
5.3.99.2
expression of cDNA in Escherichia coli
Gallus gallus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
5.3.99.2
Cibacron blue
0.00003 mM, 50% inhibition
Gallus gallus
5.3.99.2
haematin
0.00008 mM, 50% inhibition
Gallus gallus
5.3.99.2
Tributyltin acetate
0.00009 mM, 50% inhibition
Gallus gallus
5.3.99.2
tributyltin bromide
0.00003 mM, 50% inhibition
Gallus gallus
5.3.99.2
Tributyltin chloride
0.00001 mM, 50% inhibition
Gallus gallus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.3.99.2
22732
-
x * 22732, deduced from nucleotide sequence
Gallus gallus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.3.99.2
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
Gallus gallus
-
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate
-
Gallus gallus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.3.99.2
Gallus gallus
O73888
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.3.99.2
recombinant PGDS
Gallus gallus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.2
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
-
649654
Gallus gallus
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate
-
649654
Gallus gallus
?
5.3.99.2
1-bromo-2,4-dinitrobenzene + glutathione
conjugation of glutathione
649654
Gallus gallus
?
-
649654
Gallus gallus
?
5.3.99.2
1-chloro-2,4-dinitrobenzene + glutathione
conjugation of glutathione
649654
Gallus gallus
?
-
649654
Gallus gallus
?
5.3.99.2
1-fluoro-2,4-dinitrobenzene + glutathione
conjugation of glutathione
649654
Gallus gallus
?
-
649654
Gallus gallus
?
5.3.99.2
1-iodo-2,4-dinitrobenzene + glutathione
conjugation of glutathione
649654
Gallus gallus
?
-
649654
Gallus gallus
?
5.3.99.2
4-hydroxynon-2-enal + glutathione
conjugation of glutathione
649654
Gallus gallus
?
-
649654
Gallus gallus
?
5.3.99.2
4-nitrobenzyl chloride + glutathione
conjugation of glutathione
649654
Gallus gallus
?
-
649654
Gallus gallus
?
5.3.99.2
7-chloro-4-nitrobenz-2-oxa-1,3-diazole + glutathione
conjugation of glutathione
649654
Gallus gallus
?
-
649654
Gallus gallus
?
5.3.99.2
allyl isothiocyanate + glutathione
conjugation of glutathione
649654
Gallus gallus
?
-
649654
Gallus gallus
?
5.3.99.2
benzyl isothiocyanate + glutathione
conjugation of glutathione
649654
Gallus gallus
?
-
649654
Gallus gallus
?
5.3.99.2
cumene hydroperoxide
-
649654
Gallus gallus
cumene hydroxide
-
649654
Gallus gallus
?
Subunits
EC Number
Subunits
Commentary
Organism
5.3.99.2
?
x * 22732, deduced from nucleotide sequence
Gallus gallus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
5.3.99.2
glutathione
-
Gallus gallus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.2
expression of cDNA in Escherichia coli
Gallus gallus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
5.3.99.2
glutathione
-
Gallus gallus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
5.3.99.2
Cibacron blue
0.00003 mM, 50% inhibition
Gallus gallus
5.3.99.2
haematin
0.00008 mM, 50% inhibition
Gallus gallus
5.3.99.2
Tributyltin acetate
0.00009 mM, 50% inhibition
Gallus gallus
5.3.99.2
tributyltin bromide
0.00003 mM, 50% inhibition
Gallus gallus
5.3.99.2
Tributyltin chloride
0.00001 mM, 50% inhibition
Gallus gallus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.3.99.2
22732
-
x * 22732, deduced from nucleotide sequence
Gallus gallus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.3.99.2
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
Gallus gallus
-
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate
-
Gallus gallus
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.2
recombinant PGDS
Gallus gallus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.2
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
-
649654
Gallus gallus
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate
-
649654
Gallus gallus
?
5.3.99.2
1-bromo-2,4-dinitrobenzene + glutathione
conjugation of glutathione
649654
Gallus gallus
?
-
649654
Gallus gallus
?
5.3.99.2
1-chloro-2,4-dinitrobenzene + glutathione
conjugation of glutathione
649654
Gallus gallus
?
-
649654
Gallus gallus
?
5.3.99.2
1-fluoro-2,4-dinitrobenzene + glutathione
conjugation of glutathione
649654
Gallus gallus
?
-
649654
Gallus gallus
?
5.3.99.2
1-iodo-2,4-dinitrobenzene + glutathione
conjugation of glutathione
649654
Gallus gallus
?
-
649654
Gallus gallus
?
5.3.99.2
4-hydroxynon-2-enal + glutathione
conjugation of glutathione
649654
Gallus gallus
?
-
649654
Gallus gallus
?
5.3.99.2
4-nitrobenzyl chloride + glutathione
conjugation of glutathione
649654
Gallus gallus
?
-
649654
Gallus gallus
?
5.3.99.2
7-chloro-4-nitrobenz-2-oxa-1,3-diazole + glutathione
conjugation of glutathione
649654
Gallus gallus
?
-
649654
Gallus gallus
?
5.3.99.2
allyl isothiocyanate + glutathione
conjugation of glutathione
649654
Gallus gallus
?
-
649654
Gallus gallus
?
5.3.99.2
benzyl isothiocyanate + glutathione
conjugation of glutathione
649654
Gallus gallus
?
-
649654
Gallus gallus
?
5.3.99.2
cumene hydroperoxide
-
649654
Gallus gallus
cumene hydroxide
-
649654
Gallus gallus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
5.3.99.2
?
x * 22732, deduced from nucleotide sequence
Gallus gallus