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Literature summary extracted from

  • Lee, K.L.; Albee, K.L.; Bernasconi, R.J.; Edmunds, T.
    Complete amino acid sequence of ananain and a comparison with stem bromelain and other plant cysteine proteases (1997), Biochem. J., 327, 199-202.
No PubMed abstract available

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.31 23464
-
 x * 23464, amino acid sequence determination Ananas comosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.31 protein + H2O Ananas comosus
-
 peptides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.31 Ananas comosus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.22.31 hydrolysis of proteins with broad specificity for peptide bonds. Best reported small molecule substrate Bz-Phe-Val-Arg-/-NHMec, but broader specificity than fruit bromelain from stem of pineapple plant, Ananas comosus,. Differs from stem and fruit bromelains in being inhibited by chicken cystatin, amino acid sequence, contains an insert between residues 170 and 174 not present in stem bromelain or papain and a hydropobic series of amino acids adjacent to His157, which possibly contributes to the different substrate and inhibitor specificities Ananas comosus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.31 protein + H2O
-
 Ananas comosus peptides
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.31 ? x * 23464, amino acid sequence determination Ananas comosus