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Literature summary extracted from

  • Yun, N.R.; Yamamoto, M.; Arai, H.; Ishii, M.; Igarashi, Y.
    A novel five-subunit-type 2-oxoglutalate:ferredoxin oxidoreductases from Hydrogenobacter thermophilus TK-6 (2002), Biochem. Biophys. Res. Commun., 292, 280-286.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.7.3 expression in Escherichia coli JM109 Hydrogenobacter thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.7.3 2-oxoglutarate + CoA + oxidized ferredoxin Hydrogenobacter thermophilus involved in reductive TCA cycle succinyl-CoA + CO2 + reduced ferredoxin
-
 ?
1.2.7.3 2-oxoglutarate + CoA + oxidized ferredoxin Hydrogenobacter thermophilus TK-6 / IAM 12695 involved in reductive TCA cycle succinyl-CoA + CO2 + reduced ferredoxin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.3 Hydrogenobacter thermophilus D3DIA1 and D3DIA2 and D3DIA3 and D3DIA0 and Q93RA0 D3DIA1: subunit forA, D3DIA2: subunit forB, D3DIA3: subunit forG, D3DIA0: subunit forD, Q93RA0: subunit forE
-
1.2.7.3 Hydrogenobacter thermophilus TK-6 / IAM 12695 D3DIA1 and D3DIA2 and D3DIA3 and D3DIA0 and Q93RA0 D3DIA1: subunit forA, D3DIA2: subunit forB, D3DIA3: subunit forG, D3DIA0: subunit forD, Q93RA0: subunit forE
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.7.3
-
 Hydrogenobacter thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.7.3 3.34
-
 70°C, pH not specified in the publication, recombinant enzyme, oxidative decarboxylation of 2-oxoglutarate Hydrogenobacter thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.3 2-oxoglutarate + CoA + oxidized ferredoxin
-
 Hydrogenobacter thermophilus succinyl-CoA + CO2 + reduced ferredoxin
-
 ?
1.2.7.3 2-oxoglutarate + CoA + oxidized ferredoxin involved in reductive TCA cycle Hydrogenobacter thermophilus succinyl-CoA + CO2 + reduced ferredoxin
-
 ?
1.2.7.3 2-oxoglutarate + CoA + oxidized ferredoxin
-
 Hydrogenobacter thermophilus TK-6 / IAM 12695 succinyl-CoA + CO2 + reduced ferredoxin
-
 ?
1.2.7.3 2-oxoglutarate + CoA + oxidized ferredoxin involved in reductive TCA cycle Hydrogenobacter thermophilus TK-6 / IAM 12695 succinyl-CoA + CO2 + reduced ferredoxin
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.7.3 More five-subunit-structure: ForA, ForB, ForG, ForD, and ForE Hydrogenobacter thermophilus

Synonyms

EC Number Synonyms Comment Organism
1.2.7.3 forDABGE gene name, five-subunit-structure: ForA, ForB, ForG, ForD, and ForE Hydrogenobacter thermophilus
1.2.7.3 OGOR
-
 Hydrogenobacter thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.7.3 70
-
 assay at Hydrogenobacter thermophilus