EC Number | General Stability | Organism |
---|---|---|
6.4.1.3 | crude enzyme is relatively unstable | Myxococcus xanthus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.4.1.3 | CaCl2 | - |
Myxococcus xanthus | |
6.4.1.3 | CsCl | - |
Myxococcus xanthus | |
6.4.1.3 | CuCl2 | - |
Myxococcus xanthus | |
6.4.1.3 | FeSO4 | - |
Myxococcus xanthus | |
6.4.1.3 | KCl | - |
Myxococcus xanthus | |
6.4.1.3 | L-alanine | - |
Myxococcus xanthus | |
6.4.1.3 | L-phenylalanine | - |
Myxococcus xanthus | |
6.4.1.3 | L-serine | - |
Myxococcus xanthus | |
6.4.1.3 | L-tryptophan | strong inhibitor | Myxococcus xanthus | |
6.4.1.3 | L-tyrosine | - |
Myxococcus xanthus | |
6.4.1.3 | L-valine | - |
Myxococcus xanthus | |
6.4.1.3 | LiCl | - |
Myxococcus xanthus | |
6.4.1.3 | NaCl | - |
Myxococcus xanthus | |
6.4.1.3 | RbCl | - |
Myxococcus xanthus | |
6.4.1.3 | ZnCl2 | - |
Myxococcus xanthus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.4.1.3 | 0.032 | - |
propionyl-CoA | pH 7.2, 30°C | Myxococcus xanthus | |
6.4.1.3 | 0.036 | - |
ATP | pH 7.2, 30°C | Myxococcus xanthus | |
6.4.1.3 | 0.22 | - |
butyryl-CoA | pH 7.2, 30°C | Myxococcus xanthus | |
6.4.1.3 | 0.25 | - |
acetyl-CoA | pH 7.2, 30°C | Myxococcus xanthus | |
6.4.1.3 | 1.01 | - |
succinyl-CoA | pH 7.2, 30°C | Myxococcus xanthus | |
6.4.1.3 | 1.13 | - |
HCO3- | pH 7.2, 30°C | Myxococcus xanthus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.4.1.3 | Mg2+ | required for the formation of an Mg-ATP complex, highest activity between 1.5-2.0 mM (at 1.0-1.3 mM ATP) | Myxococcus xanthus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.4.1.3 | 53000 | - |
6 * 56000 + 6 * 53000, SDS-PAGE, composed of biotin carboxylase (alpha-subunit) and carboxyltransferase (beta-subunit) | Myxococcus xanthus |
6.4.1.3 | 56000 | - |
6 * 56000 + 6 * 53000, SDS-PAGE, composed of biotin carboxylase (alpha-subunit) and carboxyltransferase (beta-subunit) | Myxococcus xanthus |
6.4.1.3 | 605000 | 615000 | gel filtration | Myxococcus xanthus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.4.1.3 | ATP + propanoyl-CoA + HCO3- | Myxococcus xanthus | key enzyme in catabolic pathways for long-chain fatty acids, polyketides and branched chain amino acids | ADP + phosphate + (S)-methylmalonyl-CoA | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.4.1.3 | Myxococcus xanthus | - |
maximum activity after 40 h of development | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.4.1.3 | - |
Myxococcus xanthus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
6.4.1.3 | 14.13 | - |
purified enzyme, pH 7.2, 30°C | Myxococcus xanthus |
EC Number | Storage Stability | Organism |
---|---|---|
6.4.1.3 | 4°C, 20 mM phosphate buffer, pH 7.0, 2-4 weeks, no loss of activity | Myxococcus xanthus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.4.1.3 | ATP + acetyl-CoA + HCO3- | EC 6.4.1.2 activity | Myxococcus xanthus | ADP + phosphate + malonyl-CoA | - |
? | |
6.4.1.3 | ATP + butyryl-CoA + HCO3- | - |
Myxococcus xanthus | ADP + phosphate + ? | - |
? | |
6.4.1.3 | ATP + propanoyl-CoA + HCO3- | - |
Myxococcus xanthus | ADP + phosphate + (S)-methylmalonyl-CoA | - |
? | |
6.4.1.3 | ATP + propanoyl-CoA + HCO3- | key enzyme in catabolic pathways for long-chain fatty acids, polyketides and branched chain amino acids | Myxococcus xanthus | ADP + phosphate + (S)-methylmalonyl-CoA | - |
? | |
6.4.1.3 | ATP + succinyl-CoA + HCO3- | - |
Myxococcus xanthus | ADP + phosphate + ? | - |
? | |
6.4.1.3 | additional information | methylmalonyl-CoA, malonyl-CoA, isobutyryl-CoA, and isovaleryl-CoA are not metabolized | Myxococcus xanthus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.4.1.3 | multimer | 6 * 56000 + 6 * 53000, SDS-PAGE, composed of biotin carboxylase (alpha-subunit) and carboxyltransferase (beta-subunit) | Myxococcus xanthus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.4.1.3 | 25 | 30 | - |
Myxococcus xanthus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.4.1.3 | 7 | 7.5 | - |
Myxococcus xanthus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
6.4.1.3 | biotin | bound to alpha-subunit of the enzyme | Myxococcus xanthus |