Literature summary extracted from

Carvajal, N.; Orellana, M.S.; Salas, M.; Enriquez, P.; Alarcon, R.; Uribe, E.; Lopez, V.
Kinetic studies and site-directed mutagenesis of Escherichia coli agmatinase. A role for Glu274 in binding and correct positioning of the substrate guanidinium group (2004), Arch. Biochem. Biophys., 430, 185-190.

Protein Variants

EC Number	Protein Variants	Comment	Organism
3.5.3.11	E274A	1-2% of wild type activity	Escherichia coli

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
3.5.3.11	guanidinium ion	competitive	Escherichia coli
3.5.3.11	putrescine	competitive	Escherichia coli

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.5.3.11	1.1	-	agmatine	wild type	Escherichia coli
3.5.3.11	6.3	-	agmatine	mutant E274A	Escherichia coli

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.5.3.11	Escherichia coli	-	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.5.3.11	agmatine + H2O	-	Escherichia coli	putrescine + urea	-	?

Ki Value [mM]

EC Number	Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
3.5.3.11	15	-	guanidinium ion	wild type	Escherichia coli
3.5.3.11	44.2	-	guanidinium ion	mutant E274A	Escherichia coli

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Release 2023.1 (January 2023)