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Literature summary extracted from

  • Wu, T.K.; Huang, C.Y.; Ko, C.Y.; Chang, C.H.; Chen, Y.J.; Liao, H.K.
    Purification, tandem mass characterization, and inhibition studies of oxidosqualene-lanosterol cyclase enzyme from bovine liver (2004), Arch. Biochem. Biophys., 421, 42-53.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.4.99.7 diethyl dicarbonate maximal inactivation at neutral pH, ´reactivation by hydroxylamine Bos taurus
5.4.99.7 N,N-dimethyl-N-(4-methylenebenzophenonyl)amine IC50: 0.005 mM Bos taurus
5.4.99.7 N-(2-methylenebenzophenonyl)pyridinium IC50: 0.059 mM Bos taurus
5.4.99.7 N-(3-methylenebenzophenonyl)pyridinium bromide IC50: 0.016 mM Bos taurus
5.4.99.7 N-(4-methylenebenzophenonyl)pyridinium bromide IC50: 0.00079 mM Bos taurus
5.4.99.7 Ro48-8071 IC50: 22 nM Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.99.7 0.011
-
 (3S)-2,3-oxidosqualene
-
 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.4.99.7 microsome
-
 Bos taurus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.99.7 70000
-
 2 * 70000, SDS-PAGE Bos taurus
5.4.99.7 140000
-
 non-denaturing PAGE Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.7 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.99.7
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.4.99.7 1.747
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
5.4.99.7 -20°C or -80°C, phosphate buffer, in presence of Triton and DTT, stable for up to 6 months Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.7 (3S)-2,3-oxidosqualene two essential histidine residues per active site are necessary for complete activity Bos taurus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.4.99.7 dimer 2 * 70000, SDS-PAGE Bos taurus

Synonyms

EC Number Synonyms Comment Organism
5.4.99.7 OSC
-
 Bos taurus
5.4.99.7 Oxidosqualene-lanosterol cyclase
-
 Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.4.99.7 37
-
-
 Bos taurus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.4.99.7 25 50 optimal activity between 25°C and 50°C with a maximum at 37°C Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4.99.7 7.4
-
-
 Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.4.99.7 6 9 pH 6.0: about 20% of maximal activity, pH 7.0: about 90% of maximal activity, pH 9.0: about 50% of maximal activity Bos taurus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
5.4.99.7 0.000022
-
 IC50: 22 nM Bos taurus Ro48-8071
5.4.99.7 0.00079
-
 IC50: 0.00079 mM Bos taurus N-(4-methylenebenzophenonyl)pyridinium bromide
5.4.99.7 0.005
-
 IC50: 0.005 mM Bos taurus N,N-dimethyl-N-(4-methylenebenzophenonyl)amine
5.4.99.7 0.016
-
 IC50: 0.016 mM Bos taurus N-(3-methylenebenzophenonyl)pyridinium bromide
5.4.99.7 0.059
-
 IC50: 0.059 mM Bos taurus N-(2-methylenebenzophenonyl)pyridinium