EC Number | Cloned (Comment) | Organism |
---|---|---|
6.3.4.3 | - |
Moorella thermoacetica |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
6.3.4.3 | R97E | no activity | Moorella thermoacetica |
6.3.4.3 | R97S | 35% of wild-type kcat | Moorella thermoacetica |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.4.3 | 0.087 | - |
ATP | 27°C, pH 7.0, wild-type FTHFS | Moorella thermoacetica | |
6.3.4.3 | 0.16 | - |
ATP | 27°C, pH 7.0, R97S mutant FTHFS | Moorella thermoacetica | |
6.3.4.3 | 0.46 | - |
formate | 27°C, pH 7.0, wild-type FTHFS | Moorella thermoacetica | |
6.3.4.3 | 0.95 | - |
formate | 27°C, pH 7.0, R97S mutant FTHFS | Moorella thermoacetica |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.4.3 | ATP + formate + tetrahydrofolate | Moorella thermoacetica | - |
ADP + phosphate + 10-formyltetrahydrofolate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.3.4.3 | Moorella thermoacetica | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.3.4.3 | recombinant FTHFS | Moorella thermoacetica |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.4.3 | ADP + carbamoylphosphate + tetrahydrofolate | - |
Moorella thermoacetica | ATP + ? | - |
? | |
6.3.4.3 | ATP + formate + tetrahydrofolate | - |
Moorella thermoacetica | ADP + phosphate + 10-formyltetrahydrofolate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.3.4.3 | FTHFS | - |
Moorella thermoacetica |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.4.3 | 0.39 | - |
ATP | 27°C, pH 7.0, R97S mutant FTHFS | Moorella thermoacetica | |
6.3.4.3 | 0.4 | - |
formate | 27°C, pH 7.0, R97S mutant FTHFS | Moorella thermoacetica | |
6.3.4.3 | 0.99 | - |
ATP | 27°C, pH 7.0, wild-type FTHFS | Moorella thermoacetica | |
6.3.4.3 | 1.13 | - |
formate | 27°C, pH 7.0, wild-type FTHFS | Moorella thermoacetica | |
6.3.4.3 | 6.08 | - |
ATP | 27°C, pH 7.0, wild-type FTHFS | Moorella thermoacetica | |
6.3.4.3 | 6.08 | - |
formate | 27°C, pH 7.0, wild-type FTHFS | Moorella thermoacetica |