Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Rios, S.E.; Nowak, T.
    Role of cysteine 306 in the catalytic mechanism of Ascaris suum phosphoenolpyruvate carboxykinase (2002), Arch. Biochem. Biophys., 404, 25-37.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.32 wild-type and mutant PEPCK, expression in Escherichia coli 1786 and JM109 Ascaris suum

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.32 C306A catalytically active mutant, kinetics Ascaris suum
4.1.1.32 C306S catalytically active mutant, kinetics Ascaris suum

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.32 5,5'-dithiobis(2-nitrobenzoic acid) inhibits wild-type enzyme, but not C306A or C306S mutant enzymes Ascaris suum
4.1.1.32 additional information not inhibited by adenosine nucleotides Ascaris suum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.32 0.023
-
 GDP pH 7.4, 25°C, wild-type enzyme Ascaris suum
4.1.1.32 0.052
-
 GTP pH 7.4, 25°C, wild-type enzyme Ascaris suum
4.1.1.32 0.053
-
 2'-deoxyguanosine 5'-diphosphate pH 7.4, 25°C, wild-type enzyme Ascaris suum
4.1.1.32 0.1
-
 ITP pH 7.4, 25°C, wild-type enzyme Ascaris suum
4.1.1.32 0.11
-
 ITP pH 7.4, 25°C, C306A mutant Ascaris suum
4.1.1.32 0.12
-
 oxaloacetate pH 7.4, 25°C, wild-type enzyme Ascaris suum
4.1.1.32 0.12
-
 GDP pH 7.4, 25°C, C306S mutant Ascaris suum
4.1.1.32 0.12
-
 2'-deoxyguanosine 5'-diphosphate pH 7.4, 25°C, C306S mutant Ascaris suum
4.1.1.32 0.13
-
 GTP pH 7.4, 25°C, C306S and C306A mutant Ascaris suum
4.1.1.32 0.15
-
 GDP pH 7.4, 25°C, C306A mutant Ascaris suum
4.1.1.32 0.16
-
 ITP pH 7.4, 25°C, C306S mutant Ascaris suum
4.1.1.32 0.19
-
 2'-deoxyguanosine 5'-diphosphate pH 7.4, 25°C, C306A mutant Ascaris suum
4.1.1.32 0.19
-
 oxaloacetate pH 7.4, 25°C, C306S mutant Ascaris suum
4.1.1.32 0.2
-
 oxaloacetate pH 7.4, 25°C, C306A mutant Ascaris suum
4.1.1.32 0.3
-
 IDP pH 7.4, 25°C, wild-type enzyme Ascaris suum
4.1.1.32 0.41
-
 phosphoenolpyruvate pH 7.4, 25°C, C306S mutant Ascaris suum
4.1.1.32 0.48
-
 phosphoenolpyruvate pH 7.4, 25°C, C306A mutant Ascaris suum
4.1.1.32 0.71
-
 2'-deoxyguanosine 5'-triphosphate pH 7.4, 25°C, C306A mutant Ascaris suum
4.1.1.32 1.02
-
 2'-deoxyguanosine 5'-triphosphate pH 7.4, 25°C, C306S mutant Ascaris suum
4.1.1.32 1.05
-
 2'-deoxyguanosine 5'-triphosphate pH 7.4, 25°C, wild-type enzyme Ascaris suum
4.1.1.32 1.2
-
 IDP pH 7.4, 25°C, C306A mutant Ascaris suum
4.1.1.32 1.38
-
 IDP pH 7.4, 25°C, C306S mutant Ascaris suum
4.1.1.32 2.42
-
 phosphoenolpyruvate pH 7.4, 25°C, wild-type enzyme Ascaris suum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.32 Mn2+ enzyme binds Mn2+ at the catalytic site, binary PEPCK-Mn2+ complex, activator Mn2+ enhances the nucleotide binding Ascaris suum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.32 GTP + oxaloacetate Ascaris suum
-
 GDP + phosphoenolpyruvate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.32 Ascaris suum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.32 recombinant and mutant enzyme Ascaris suum

Reaction

EC Number Reaction Comment Organism Reaction ID
4.1.1.32 GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2 catalytic mechanism Ascaris suum
a

Storage Stability

EC Number Storage Stability Organism
4.1.1.32 4°C, 3 weeks, stable Ascaris suum
4.1.1.32 room temperature, at least 6 h, stable Ascaris suum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.32 2'-deoxyguanosine 5'-triphosphate + oxaloacetate 2’-deoxyGTP is a less effective substrate than GTP, 2’-deoxyGTP binds to enzyme less tight than 2’-deoxyGDP Ascaris suum 2'-deoxyguanosine 5'-diphosphate + phosphoenolpyruvate + CO2 2’-deoxyGDP is a less effective substrate than GDP in the reverse reaction ?
4.1.1.32 GTP + oxaloacetate
-
 Ascaris suum GDP + phosphoenolpyruvate + CO2
-
 ?
4.1.1.32 GTP + oxaloacetate Cys-306 is important in nucleotide binding and may interact with the 2’-OH group in the ribose ring. GTP binds to enzyme tighter than GDP Ascaris suum GDP + phosphoenolpyruvate + CO2 GDP is a slightly more favorable substrate than IDP in the reverse reaction r
4.1.1.32 ITP + oxaloacetate
-
 Ascaris suum IDP + phosphoenolpyruvate + CO2 GDP is a slightly more favorable substrate than IDP in the reverse reaction r
4.1.1.32 additional information not: adenosine nucleotides Ascaris suum ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.32 25
-
 assay at Ascaris suum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.32 0.4
-
 2'-deoxyguanosine 5'-diphosphate pH 7.4, 25°C, C306S mutant Ascaris suum
4.1.1.32 0.5
-
 2'-deoxyguanosine 5'-diphosphate pH 7.4, 25°C, C306A mutant Ascaris suum
4.1.1.32 1.8
-
 phosphoenolpyruvate pH 7.4, 25°C, C306A mutant Ascaris suum
4.1.1.32 2.1
-
 phosphoenolpyruvate pH 7.4, 25°C, C306S mutant Ascaris suum
4.1.1.32 2.3
-
 2'-deoxyguanosine 5'-triphosphate pH 7.4, 25°C, C306A mutant Ascaris suum
4.1.1.32 2.3
-
 IDP pH 7.4, 25°C, C306S mutant Ascaris suum
4.1.1.32 2.4
-
 IDP pH 7.4, 25°C, C306A mutant Ascaris suum
4.1.1.32 2.4
-
 2'-deoxyguanosine 5'-triphosphate pH 7.4, 25°C, C306S mutant Ascaris suum
4.1.1.32 3.4
-
 2'-deoxyguanosine 5'-triphosphate pH 7.4, 25°C, wild-type enzyme Ascaris suum
4.1.1.32 3.5
-
 GDP pH 7.4, 25°C, C306S mutant Ascaris suum
4.1.1.32 4.1
-
 GDP pH 7.4, 25°C, C306A mutant Ascaris suum
4.1.1.32 4.6
-
 2'-deoxyguanosine 5'-diphosphate pH 7.4, 25°C, wild-type enzyme Ascaris suum
4.1.1.32 6.5
-
 GDP pH 7.4, 25°C, wild-type enzyme Ascaris suum
4.1.1.32 6.9
-
 IDP pH 7.4, 25°C, wild-type enzyme Ascaris suum
4.1.1.32 9.3
-
 phosphoenolpyruvate pH 7.4, 25°C, wild-type enzyme Ascaris suum
4.1.1.32 10.3
-
 GTP pH 7.4, 25°C, C306S mutant Ascaris suum
4.1.1.32 10.7
-
 ITP pH 7.4, 25°C, C306A mutant Ascaris suum
4.1.1.32 12.4
-
 GTP pH 7.4, 25°C, C306A mutant Ascaris suum
4.1.1.32 12.8
-
 ITP pH 7.4, 25°C, C306S mutant Ascaris suum
4.1.1.32 13.3
-
 oxaloacetate pH 7.4, 25°C, C306S mutant Ascaris suum
4.1.1.32 13.9
-
 GTP pH 7.4, 25°C, wild-type enzyme Ascaris suum
4.1.1.32 14.5
-
 oxaloacetate pH 7.4, 25°C, C306A mutant Ascaris suum
4.1.1.32 15.6
-
 ITP pH 7.4, 25°C, wild-type enzyme Ascaris suum
4.1.1.32 17.8
-
 oxaloacetate pH 7.4, 25°C, wild-type enzyme Ascaris suum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.32 7.4
-
 assay at Ascaris suum