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Literature summary extracted from

  • Pierrat, O.A.; Javid-Majd, F.; Raushel, F.M.
    Dissection of the conduit for allosteric control of carbamoyl phosphate synthetase by ornithine (2002), Arch. Biochem. Biophys., 400, 26-33.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.5.5 IMP allosteric effector Escherichia coli
6.3.5.5 L-ornithine positive allosteric activation, E783, T1042, and T1043 are primarily responsible for the binding of ornithine to enzyme Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
6.3.5.5 E761A the residue is a key in the allosteric signal transduction pathway from ornithine to ATP Escherichia coli
6.3.5.5 E783A the allosteric activation of enzyme by ornithine is completely suppressed Escherichia coli
6.3.5.5 E783K the allosteric activation of enzyme by ornithine is completely suppressed Escherichia coli
6.3.5.5 E892A the allosteric activation of enzyme by ornithine is completely suppressed Escherichia coli
6.3.5.5 E892K the allosteric activation of enzyme by ornithine is completely suppressed Escherichia coli
6.3.5.5 additional information several mutations increase, reduce, suppresse, or reverse the allosteric effects of ornithine, UMP and IMP, the mutational analysis of ornithine, and potassium binding site strongly supports the proposal for a coupling between the allosteric transduction signal pathways for UMP,IMP and ornithine Escherichia coli
6.3.5.5 T1042K the residue is responsible for the binding of ornithine to enzyme Escherichia coli
6.3.5.5 T1043K the allosteric activation of enzyme by ornithine is completely suppressed Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.5.5 UMP
-
Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.5 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.5 2 ATP + L-Gln + HCO3-
-
Escherichia coli 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
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Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.5.5 additional information
-
additional information comparison of kcat in absence and presence of L-ornithine, UMP and IMP for wild-type and mutant enzymes Escherichia coli