Literature summary extracted from

Walker, N.; Holley, J.; Naylor, C.E.; Flores-Diaz, M.; Alape-Giron, A.; Carter, G.; Carr, F.J.; Thelestam, M.; Keyte, M.; Moss, D.S.; Basak, A.K.; Miller, J.; Titball, R.W.
identification of residues in the carboxy-terminal domain of Clostridium perfringens alpha-toxin (phospholipase C) which are required for its biological activities (2000), Arch. Biochem. Biophys., 384, 24-30.

Protein Variants

EC Number	Protein Variants	Comment	Organism
3.1.4.3	D293S	reduced hemolytic activity, reduced cytotoxic and myotoxic activities, reduced activity against p-nitrophenylphosphorylcholine, increased dependence on Ca2+	Clostridium perfringens
3.1.4.3	D305G	reduced hemolytic activity, reduced cytotoxic and myotoxic activities, significantly increased activity against p-nitrophenylphosphorylcholine	Clostridium perfringens
3.1.4.3	K330E	reduced hemolytic activity, reduced cytotoxic and myotoxic activities	Clostridium perfringens

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.1.4.3	Clostridium perfringens	-	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.1.4.3	phosphatidylcholine + H2O	-	Clostridium perfringens	1,2-diacylglycerol + phosphorylcholine	-	?

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Release 2023.1 (January 2023)