BRENDA - Enzyme Database show

Identification of thromboxane synthase amino acid residues involved in heme-propionate binding

Hsu, P.Y.; Tsai, A.L.; Wang, L.H.; Arch. Biochem. Biophys. 383, 119-127 (2000)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
5.3.99.5
expression in Escherichia coli
Homo sapiens
Engineering
EC Number
Amino acid exchange
Commentary
Organism
5.3.99.5
A408L
42% of wild-type activity
Homo sapiens
5.3.99.5
A415V
82% of wild-type activity
Homo sapiens
5.3.99.5
C480H
no activity
Homo sapiens
5.3.99.5
C480S
no activity
Homo sapiens
5.3.99.5
C480Y
no activity
Homo sapiens
5.3.99.5
E134D
35% of wild-type activity
Homo sapiens
5.3.99.5
E135D
123% of wild-type activity
Homo sapiens
5.3.99.5
E414D
39% of wild-type activity
Homo sapiens
5.3.99.5
F409Y
42% of wild-type activity
Homo sapiens
5.3.99.5
F411Y
38% of wild-type activity
Homo sapiens
5.3.99.5
N110I
no activity
Homo sapiens
5.3.99.5
R137A
2% of wild-type activity
Homo sapiens
5.3.99.5
R137K
5% of wild-type activity
Homo sapiens
5.3.99.5
R410G
46% of wild-type activity
Homo sapiens
5.3.99.5
R413K
1% of wild-type activity
Homo sapiens
5.3.99.5
R478A
no activity
Homo sapiens
5.3.99.5
T412S
41% of wild-type activity
Homo sapiens
5.3.99.5
V136A
69% of wild-type activity
Homo sapiens
5.3.99.5
W133F
2% of wild-type activity
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.99.5
endoplasmic reticulum
-
Homo sapiens
5783
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.3.99.5
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
Homo sapiens
-
(5Z,13E)-(15S)-9alpha,11alpha-epoxy-15-hydroxythromboxa-5,13-dienoate
i.e. TXA2
Homo sapiens
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.3.99.5
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.3.99.5
recombinant TXAS
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.5
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
-
649437
Homo sapiens
(5Z,13E)-(15S)-9alpha,11alpha-epoxy-15-hydroxythromboxa-5,13-dienoate
i.e. TXA2
649437
Homo sapiens
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.5
expression in Escherichia coli
Homo sapiens
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
5.3.99.5
A408L
42% of wild-type activity
Homo sapiens
5.3.99.5
A415V
82% of wild-type activity
Homo sapiens
5.3.99.5
C480H
no activity
Homo sapiens
5.3.99.5
C480S
no activity
Homo sapiens
5.3.99.5
C480Y
no activity
Homo sapiens
5.3.99.5
E134D
35% of wild-type activity
Homo sapiens
5.3.99.5
E135D
123% of wild-type activity
Homo sapiens
5.3.99.5
E414D
39% of wild-type activity
Homo sapiens
5.3.99.5
F409Y
42% of wild-type activity
Homo sapiens
5.3.99.5
F411Y
38% of wild-type activity
Homo sapiens
5.3.99.5
N110I
no activity
Homo sapiens
5.3.99.5
R137A
2% of wild-type activity
Homo sapiens
5.3.99.5
R137K
5% of wild-type activity
Homo sapiens
5.3.99.5
R410G
46% of wild-type activity
Homo sapiens
5.3.99.5
R413K
1% of wild-type activity
Homo sapiens
5.3.99.5
R478A
no activity
Homo sapiens
5.3.99.5
T412S
41% of wild-type activity
Homo sapiens
5.3.99.5
V136A
69% of wild-type activity
Homo sapiens
5.3.99.5
W133F
2% of wild-type activity
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.99.5
endoplasmic reticulum
-
Homo sapiens
5783
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.3.99.5
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
Homo sapiens
-
(5Z,13E)-(15S)-9alpha,11alpha-epoxy-15-hydroxythromboxa-5,13-dienoate
i.e. TXA2
Homo sapiens
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.5
recombinant TXAS
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.5
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
-
649437
Homo sapiens
(5Z,13E)-(15S)-9alpha,11alpha-epoxy-15-hydroxythromboxa-5,13-dienoate
i.e. TXA2
649437
Homo sapiens
?