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Literature summary extracted from

  • Swamy, K.H.; Chung, C.H.; Goldberg, A.L.
    Isolation and characterization of protease do from Escherichia coli, a large serine protease containing multiple subunits (1983), Arch. Biochem. Biophys., 224, 543-554.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.107 diisopropyl fluorophosphate binds covalently to the enzyme Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.107 cytoplasm
-
 Escherichia coli 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.107 54000
-
 10 * 54000, SDS-PAGE, enzyme exists in 3 different forms: pentamer, hexamer and decamer Escherichia coli
3.4.21.107 54000
-
 5 * 54000, SDS-PAGE, enzyme exists in 3 different forms: pentamer, hexamer and decamer Escherichia coli
3.4.21.107 54000
-
 6 * 54000, SDS-PAGE, enzyme exists in 3 different forms: pentamer, hexamer and decamer Escherichia coli
3.4.21.107 300000
-
 gel filtration, 2 forms: 300000 Da and 500000 Da Escherichia coli
3.4.21.107 500000
-
 gel filtration, 2 forms: 300000 Da and 500000 Da Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.107 additional information Escherichia coli involved in the degradation of damaged proteins ?
-
 ?
3.4.21.107 Protein + H2O Escherichia coli
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.107 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.107 near homogeneity Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.107 Bovine serum albumin + H2O denatured Escherichia coli ?
-
 ?
3.4.21.107 casein + H2O
-
 Escherichia coli ?
-
 ?
3.4.21.107 Globin + H2O
-
 Escherichia coli ?
-
 ?
3.4.21.107 additional information no substrates are: native bovine serum albumin, insulin, growth hormone or a variety of commonly used peptide substrates Escherichia coli ?
-
 ?
3.4.21.107 additional information involved in the degradation of damaged proteins Escherichia coli ?
-
 ?
3.4.21.107 Protein + H2O
-
 Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.107 decamer 10 * 54000, SDS-PAGE, enzyme exists in 3 different forms: pentamer, hexamer and decamer Escherichia coli
3.4.21.107 hexamer 6 * 54000, SDS-PAGE, enzyme exists in 3 different forms: pentamer, hexamer and decamer Escherichia coli
3.4.21.107 pentamer 5 * 54000, SDS-PAGE, enzyme exists in 3 different forms: pentamer, hexamer and decamer Escherichia coli