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Literature summary extracted from

  • Velasco, J.; Gutierrez, S.; Casqueiro, J.; Fierro, F.; Campoy, S.; Martin, J.F.
    Cloning and characterization of the gene cahB encoding a cephalosporin C acetylhydrolase from Acremonium chrysogenum (2001), Appl. Microbiol. Biotechnol., 57, 350-356.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.41 diisopropylfluorophosphate complete inhibition at 10 mM Acremonium chrysogenum
3.1.1.41 phenylmethylsulfonyl fluoride complete inhibition at 10 mM Acremonium chrysogenum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.41 33.7
-
 cephalosporin C pH 8, 37°C Acremonium chrysogenum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.41 extracellular
-
 Acremonium chrysogenum
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.41 31000
-
 SDS-PAGE, gel filtration Acremonium chrysogenum
3.1.1.41 38230
-
 preprotein, calculated from amino acid sequence Acremonium chrysogenum

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.41 Acremonium chrysogenum
-
 C10
-
3.1.1.41 Acremonium chrysogenum C10 / ATCC 48272
-
 C10
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.41 proteolytic modification preprotein is cleaved to the mature enzyme Acremonium chrysogenum

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.41 near homogeneity Acremonium chrysogenum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.41 4-nitrophenyl acetate + H2O
-
 Acremonium chrysogenum 4-nitrophenol + acetate
-
 ?
3.1.1.41 4-nitrophenyl acetate + H2O
-
 Acremonium chrysogenum C10 / ATCC 48272 4-nitrophenol + acetate
-
 ?
3.1.1.41 cephalosporin C + H2O
-
 Acremonium chrysogenum deacetylcephalosporin C + acetate
-
 ?
3.1.1.41 cephalosporin C + H2O
-
 Acremonium chrysogenum C10 / ATCC 48272 deacetylcephalosporin C + acetate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.41 CPC-AH
-
 Acremonium chrysogenum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.41 45
-
-
 Acremonium chrysogenum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.41 5 65 significant activity within this range Acremonium chrysogenum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.41 8
-
-
 Acremonium chrysogenum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.41 4 11 significant activity within this range Acremonium chrysogenum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.41 Acremonium chrysogenum isoelectric focusing, pH gradient 3-10
-
 4