Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Reiter, B.; Glieder, A.; Talker, D.; Schwab, H.
    Cloning and characterization of EstC from Burkholderia gladioli, a novel-type esterase related to plant enzymes (2000), Appl. Microbiol. Biotechnol., 54, 778-785.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.1 DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli BL21(DE3) Burkholderia gladioli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.1 32000
-
 x * 32000, SDS-PAGE Burkholderia gladioli

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Burkholderia gladioli Q9LAB8 gene EstC, formerly Pseudomonas marginata
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.1 recombinant from Escherichia coli Burkholderia gladioli

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.1.1 a carboxylic ester + H2O = an alcohol + a carboxylate catalytic triad near the C-terminus includes His275 Burkholderia gladioli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 4-methylumbelliferyl butyrate + H2O high activity Burkholderia gladioli 4-methylumbelliferol + butyrate
-
 ?
3.1.1.1 7-acetoxy-3-(2-benzothiazolyl)-4-cyanocoumarin + H2O low activity Burkholderia gladioli ?
-
 ?
3.1.1.1 alpha-naphthyl acetate + H2O
-
 Burkholderia gladioli alpha-naphthol + acetate
-
 ?
3.1.1.1 alpha-naphthyl butyrate + H2O
-
 Burkholderia gladioli alpha-naphthol + butyrate
-
 ?
3.1.1.1 alpha-naphthyl nonanoate + H2O low activity Burkholderia gladioli alpha-naphthol + nonanoate
-
 ?
3.1.1.1 alpha-naphthyl propionate + H2O best substrate Burkholderia gladioli 1-naphthol + propionate
-
 ?
3.1.1.1 alpha-naphthyl valerate + H2O
-
 Burkholderia gladioli alpha-naphthol + valerate
-
 ?
3.1.1.1 beta-naphthyl butyrate + H2O
-
 Burkholderia gladioli beta-naphthol + butyrate
-
 ?
3.1.1.1 beta-naphthyl caproate + H2O low activity Burkholderia gladioli beta-naphthol + caproate
-
 ?
3.1.1.1 beta-naphthyl propionate + H2O best substrate Burkholderia gladioli 2-naphthol + propionate
-
 ?
3.1.1.1 beta-naphthyl valerate + H2O
-
 Burkholderia gladioli beta-naphthol + valerate
-
 ?
3.1.1.1 additional information no activity with alpha-naphthol palmitate, substrate specificity, activity with other nitrophenyl esters, naphthol-AS esters, and HPTS esters, overview Burkholderia gladioli ?
-
 ?
3.1.1.1 resorufine-butyrate + H2O low activity Burkholderia gladioli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.1 ? x * 32000, SDS-PAGE Burkholderia gladioli

Synonyms

EC Number Synonyms Comment Organism
3.1.1.1 EstC
-
 Burkholderia gladioli