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Literature summary extracted from

  • Cardoza, R.E.; Velasco, J.; Martin, J.F.; Liras, P.
    A cephalosporin C acetylhydrolase is present in the cultures of Nocardia lactamdurans (2000), Appl. Microbiol. Biotechnol., 54, 406-412.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.41 AsO43- 40% inhibition at 1 mM Amycolatopsis lactamdurans
3.1.1.41 diisopropylfluorophosphate complete inhibition at 0.5 mM Amycolatopsis lactamdurans
3.1.1.41 dimethylphosphite slight inhibition Amycolatopsis lactamdurans
3.1.1.41 EDTA slight inhibition Amycolatopsis lactamdurans
3.1.1.41 trimethylphosphite slight inhibition Amycolatopsis lactamdurans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.41 7
-
 cephalosporin C pH 8, 37°C Amycolatopsis lactamdurans
3.1.1.41 8.3
-
 7-aminocephalosporanic acid pH 8, 37°C Amycolatopsis lactamdurans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.41 extracellular
-
 Amycolatopsis lactamdurans
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.41 72100
-
 gel filtration Amycolatopsis lactamdurans

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.41 Amycolatopsis lactamdurans
-
 and strain LC411
-
3.1.1.41 Amycolatopsis lactamdurans MA4213
-
 and strain LC411
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.41 homogeneity Amycolatopsis lactamdurans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.41 mycelium
-
 Amycolatopsis lactamdurans
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.41 205
-
-
 Amycolatopsis lactamdurans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.41 4-nitrophenyl acetate + H2O
-
 Amycolatopsis lactamdurans 4-nitrophenol + acetate
-
 ?
3.1.1.41 4-nitrophenyl acetate + H2O
-
 Amycolatopsis lactamdurans MA4213 4-nitrophenol + acetate
-
 ?
3.1.1.41 7-aminocephalosporanic acid + H2O
-
 Amycolatopsis lactamdurans acetate + deacetyl-7-aminocephalosporanic acid
-
 ?
3.1.1.41 7-aminocephalosporanic acid + H2O
-
 Amycolatopsis lactamdurans MA4213 acetate + deacetyl-7-aminocephalosporanic acid
-
 ?
3.1.1.41 cephalosporin C + H2O
-
 Amycolatopsis lactamdurans deacetylcephalosporin C + acetate
-
 ?
3.1.1.41 cephalosporin C + H2O
-
 Amycolatopsis lactamdurans MA4213 deacetylcephalosporin C + acetate
-
 ?
3.1.1.41 additional information no substrate: cephamycin C Amycolatopsis lactamdurans ?
-
 ?
3.1.1.41 additional information no substrate: cephamycin C Amycolatopsis lactamdurans MA4213 ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.41 45
-
 50% loss of activity after 1 h Amycolatopsis lactamdurans