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Literature summary extracted from

  • Bolumar, T.; Sanz, Y.; Aristoy, M.C.; Toldra, F.
    Purification and characterization of a prolyl aminopeptidase from Debaryomyces hansenii (2003), Appl. Environ. Microbiol., 69, 227-232.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.5 4-chloromercuribenzoic acid 61% remaining activity at 1 mM Debaryomyces hansenii
3.4.11.5 Hg2+
-
 Debaryomyces hansenii
3.4.11.5 iodoacetate 2.6% remaining activity at 1 mM Debaryomyces hansenii
3.4.11.5 additional information not affected by bestatin, puromycin, reducing agents, chelating agents and cations Debaryomyces hansenii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.5 0.04
-
 proline-7-amido-4-methylcoumarin pH 7.5, 37°C Debaryomyces hansenii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.5 53500
-
 7 * 53500, SDS-PAGE Debaryomyces hansenii
3.4.11.5 370000
-
 gel filtration Debaryomyces hansenii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.11.5 additional information Debaryomyces hansenii enzyme possibly plays a role in meat fermentation ?
-
 ?
3.4.11.5 additional information Debaryomyces hansenii CECT12487 enzyme possibly plays a role in meat fermentation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.5 Debaryomyces hansenii
-
-
-
3.4.11.5 Debaryomyces hansenii CECT12487
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.5 248fold Debaryomyces hansenii

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.11.5 release of N-terminal proline from a peptide the enzyme exclusively hydrolyzes N-terminal-proline-containing substrates Debaryomyces hansenii
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.5 1.06
-
 purified enzyme Debaryomyces hansenii

Storage Stability

EC Number Storage Stability Organism
3.4.11.5 4°C, purified enzyme, stable for at least 3 months Debaryomyces hansenii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.5 additional information substrate specificity, no activity with any other Xaa-7-amido-4-methylcoumarin substrate, no activity with substrates Leu-Pro, Leu-Leu, Val-Val, Gly-Pro, Lys-Lys, Glu-Glu, and Ala-Ala-Ala Debaryomyces hansenii ?
-
 ?
3.4.11.5 additional information enzyme possibly plays a role in meat fermentation Debaryomyces hansenii ?
-
 ?
3.4.11.5 additional information substrate specificity, no activity with any other Xaa-7-amido-4-methylcoumarin substrate, no activity with substrates Leu-Pro, Leu-Leu, Val-Val, Gly-Pro, Lys-Lys, Glu-Glu, and Ala-Ala-Ala Debaryomyces hansenii CECT12487 ?
-
 ?
3.4.11.5 additional information enzyme possibly plays a role in meat fermentation Debaryomyces hansenii CECT12487 ?
-
 ?
3.4.11.5 Pro-Gly + H2O
-
 Debaryomyces hansenii Pro + Gly
-
 ?
3.4.11.5 Pro-Gly + H2O
-
 Debaryomyces hansenii CECT12487 Pro + Gly
-
 ?
3.4.11.5 Pro-Leu + H2O
-
 Debaryomyces hansenii Pro + Leu
-
 ?
3.4.11.5 Pro-Leu + H2O
-
 Debaryomyces hansenii CECT12487 Pro + Leu
-
 ?
3.4.11.5 Pro-Phe + H2O
-
 Debaryomyces hansenii Pro + Phe
-
 ?
3.4.11.5 Pro-Phe + H2O
-
 Debaryomyces hansenii CECT12487 Pro + Phe
-
 ?
3.4.11.5 Pro-Phe-Gly-Lys + H2O
-
 Debaryomyces hansenii Pro + Phe-Gly-Lys
-
 ?
3.4.11.5 proline-7-amido-4-methylcoumarin + H2O
-
 Debaryomyces hansenii proline + 7-amino-4-methylcoumarin
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.5 heptamer 7 * 53500, SDS-PAGE Debaryomyces hansenii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.5 45
-
-
 Debaryomyces hansenii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.5 7.5
-
-
 Debaryomyces hansenii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.11.5 5 9.5
-
 Debaryomyces hansenii