EC Number | Cloned (Comment) | Organism |
---|---|---|
1.13.11.6 | expression in Escherichia coli | Pseudomonas fluorescens |
4.1.1.45 | nbaD gene overexpressed in Escherichia coli | Pseudomonas fluorescens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.13.11.6 | H52A | inactive enzyme | Pseudomonas fluorescens |
1.13.11.6 | H93A | 24.8% activity of the native enzyme | Pseudomonas fluorescens |
1.13.11.6 | H96A | inactive enzyme | Pseudomonas fluorescens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.6 | 1,10-phenanthroline | Fe2+ chelator, 1 mM, complete inhibition | Pseudomonas fluorescens | |
1.13.11.6 | 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide methiodide | carboxyl-directed reagent, 1 mM, 26% inhibition | Pseudomonas fluorescens | |
1.13.11.6 | 2,2'-dipyridyl | Fe2+ chelator, 1 mM, complete inhibition | Pseudomonas fluorescens | |
1.13.11.6 | Cd2+ | 0.1 mM, more than 99% inhibition | Pseudomonas fluorescens | |
1.13.11.6 | Co2+ | 1 mM, 92% inhibition | Pseudomonas fluorescens | |
1.13.11.6 | Cu2+ | 0.1 mM, complete inhibition | Pseudomonas fluorescens | |
1.13.11.6 | diethyl dicarbonate | modifies histidine residues of catechol dioxygenases, 1 mM, 70% inhibition | Pseudomonas fluorescens | |
1.13.11.6 | Dithionitrobenzoic acid | cysteine-directed reagent, 1 mM, complete inhibition | Pseudomonas fluorescens | |
1.13.11.6 | EDTA | 1 mM, 99% inhibition | Pseudomonas fluorescens | |
1.13.11.6 | Fe3+ | 0.1 mM, 82% inhibition | Pseudomonas fluorescens | |
1.13.11.6 | iodoacetate | cysteine-directed reagent, 1 mM, 42% inhibition | Pseudomonas fluorescens | |
1.13.11.6 | Ni2+ | 1 mM, 82% inhibition | Pseudomonas fluorescens | |
1.13.11.6 | Zn2+ | 0.1 mM, 99% inhibition | Pseudomonas fluorescens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.6 | Fe2+ | needed for catalytic activity | Pseudomonas fluorescens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.13.11.6 | 21240 | - |
predicted from cDNA sequence | Pseudomonas fluorescens |
1.13.11.6 | 23800 | - |
SDS-PAGE | Pseudomonas fluorescens |
4.1.1.45 | 37140 | - |
deduced from DNA sequence | Pseudomonas fluorescens |
4.1.1.45 | 38700 | - |
recombinant protein, SDS-PAGE | Pseudomonas fluorescens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.6 | 3-hydroxyanthranilate + O2 | Pseudomonas fluorescens | - |
2-amino-3-carboxymuconate semialdehyde | - |
? | |
1.13.11.6 | 3-hydroxyanthranilate + O2 | Pseudomonas fluorescens KU-7 | - |
2-amino-3-carboxymuconate semialdehyde | - |
? | |
4.1.1.45 | 2-amino-3-(3-oxoprop-1-en-1-yl)but-2-enedioate | Pseudomonas fluorescens | 2-nitrobenzoate degradation pathway | 2-aminomuconate-6-semialdehyde + CO2 | - |
? | |
4.1.1.45 | 2-amino-3-(3-oxoprop-1-en-1-yl)but-2-enedioate | Pseudomonas fluorescens KU-7 | 2-nitrobenzoate degradation pathway | 2-aminomuconate-6-semialdehyde + CO2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.11.6 | Pseudomonas fluorescens | Q83V26 | - |
- |
1.13.11.6 | Pseudomonas fluorescens KU-7 | Q83V26 | - |
- |
4.1.1.45 | Pseudomonas fluorescens | Q83V25 | - |
- |
4.1.1.45 | Pseudomonas fluorescens KU-7 | Q83V25 | - |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.13.11.6 | 7.9 | - |
3-hydroxyanthranilate as substrate, pH 6.5, 30ºC | Pseudomonas fluorescens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.6 | 3-hydroxyanthranilate + O2 | - |
Pseudomonas fluorescens | 2-amino-3-carboxymuconate semialdehyde | - |
? | |
1.13.11.6 | 3-hydroxyanthranilate + O2 | - |
Pseudomonas fluorescens KU-7 | 2-amino-3-carboxymuconate semialdehyde | - |
? | |
1.13.11.6 | additional information | no detectable activity with 3-amino-4-hydroxybenzoic acid, 4-aminoresorcinol, 2-amino-m-cresol, 4-amino-3-hydroxybenzoic acid, 3-aminosalicylic acid, 6-amino-m-cresol, 3-methylcatechol, 4-methylcatechol, 1,2,4-trihydroxybenzene, 2,3-dihydroxybenzoic acid, 4-amino-m-cresol, 5-aminosalicylic acid, gentisic acid, homogentisic acid, 2-amino-4-chlorophenol, 2-amino-p-cresol, catechol, 1,2,3-trihydroxybenzene, protocatechuic acid, hydroquinone as substrates | Pseudomonas fluorescens | ? | - |
? | |
1.13.11.6 | additional information | no detectable activity with 3-amino-4-hydroxybenzoic acid, 4-aminoresorcinol, 2-amino-m-cresol, 4-amino-3-hydroxybenzoic acid, 3-aminosalicylic acid, 6-amino-m-cresol, 3-methylcatechol, 4-methylcatechol, 1,2,4-trihydroxybenzene, 2,3-dihydroxybenzoic acid, 4-amino-m-cresol, 5-aminosalicylic acid, gentisic acid, homogentisic acid, 2-amino-4-chlorophenol, 2-amino-p-cresol, catechol, 1,2,3-trihydroxybenzene, protocatechuic acid, hydroquinone as substrates | Pseudomonas fluorescens KU-7 | ? | - |
? | |
4.1.1.45 | 2-amino-3-(3-oxoprop-1-en-1-yl)but-2-enedioate | - |
Pseudomonas fluorescens | 2-aminomuconate-6-semialdehyde + CO2 | - |
? | |
4.1.1.45 | 2-amino-3-(3-oxoprop-1-en-1-yl)but-2-enedioate | 2-nitrobenzoate degradation pathway | Pseudomonas fluorescens | 2-aminomuconate-6-semialdehyde + CO2 | - |
? | |
4.1.1.45 | 2-amino-3-(3-oxoprop-1-en-1-yl)but-2-enedioate | - |
Pseudomonas fluorescens KU-7 | 2-aminomuconate-6-semialdehyde + CO2 | - |
? | |
4.1.1.45 | 2-amino-3-(3-oxoprop-1-en-1-yl)but-2-enedioate | 2-nitrobenzoate degradation pathway | Pseudomonas fluorescens KU-7 | 2-aminomuconate-6-semialdehyde + CO2 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.1.45 | 2-amino-3-carboxymuconate-6-semialdehyde decarboxylase | - |
Pseudomonas fluorescens |
4.1.1.45 | NbaD enzyme | - |
Pseudomonas fluorescens |