EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.4.11.6 | EDTA | 0.1 mM, activation to 128% of control. 1.0 mM, activation to 146% of control | Latilactobacillus sakei |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.11.6 | 3,4-dichloroisocoumarin | 0.1 mM, 12% inhibition. 1.0 mM, 16% inhibition | Latilactobacillus sakei | |
3.4.11.6 | Ca2+ | 0.1 mM CaCl2, 19% inhibition | Latilactobacillus sakei | |
3.4.11.6 | Co2+ | 0.1 mM CoCl2, 23% inhibition | Latilactobacillus sakei | |
3.4.11.6 | Cu2+ | 0.1 mM CuCl2, complete inhibition | Latilactobacillus sakei | |
3.4.11.6 | Hg2+ | 0.1 mM HgCl2, complete inhibition | Latilactobacillus sakei | |
3.4.11.6 | iodoacetate | 0.1 mM, complete inactivation | Latilactobacillus sakei | |
3.4.11.6 | MgCl2 | 0.1 mM MgCl2, 22% inhibition | Latilactobacillus sakei | |
3.4.11.6 | Zn2+ | 0.1 mM ZnCl2, 82% inhibition | Latilactobacillus sakei |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.11.6 | 0.0159 | - |
Arg-7-amido-4-methylcoumarin | pH 5.0, 37°C | Latilactobacillus sakei | |
3.4.11.6 | 0.026 | - |
Lys-7-amido-4-methylcoumarin | pH 5.0, 37°C | Latilactobacillus sakei |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.11.6 | 60000 | - |
3 * 60000, SDS-PAGE | Latilactobacillus sakei |
3.4.11.6 | 180000 | - |
gel filtration | Latilactobacillus sakei |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.11.6 | Latilactobacillus sakei | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.11.6 | - |
Latilactobacillus sakei |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.11.6 | 1.17 | - |
- |
Latilactobacillus sakei |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.11.6 | Arg-4-nitroanilide + H2O | as active as Arg-7-amido-4-methylcoumarin | Latilactobacillus sakei | Arg + 4-nitroaniline | - |
? | |
3.4.11.6 | Arg-7-amido-4-methylcoumarin + H2O | - |
Latilactobacillus sakei | Arg + 7-amino-4-methylcoumarin | - |
? | |
3.4.11.6 | Arg-Ala + H2O | 82% of the activity with Arg-Arg | Latilactobacillus sakei | Arg + Ala | - |
? | |
3.4.11.6 | Arg-Arg + H2O | - |
Latilactobacillus sakei | Arg + Arg | - |
? | |
3.4.11.6 | Arg-Asp + H2O | 25% of the activity with Arg-Arg | Latilactobacillus sakei | Arg + Asp | - |
? | |
3.4.11.6 | Arg-Ile + H2O | 32% of the activity with Arg-Arg | Latilactobacillus sakei | Arg + Ile | - |
? | |
3.4.11.6 | Arg-Leu + H2O | 25% of the activity with Arg-Arg | Latilactobacillus sakei | Arg + Leu | - |
? | |
3.4.11.6 | Arg-Lys + H2O | 88% of the activity with Arg-Arg | Latilactobacillus sakei | Arg + Lys | - |
? | |
3.4.11.6 | Arg-Phe + H2O | 35% of the activity with Arg-Arg | Latilactobacillus sakei | Arg + Phe | - |
? | |
3.4.11.6 | Lys-4-nitroanilide + H2O | 3.6% of the activity with Arg-7-amido-4-methylcoumarin | Latilactobacillus sakei | Lys + 4-nitroaniline | - |
? | |
3.4.11.6 | Lys-7-amido-4-methylcoumarin + H2O | 4.3% of the activity with Arg-7-amido-4-methylcoumarin | Latilactobacillus sakei | Lys + 7-amino-4-methylcoumarin | - |
? | |
3.4.11.6 | Lys-Ala + H2O | 88% of the activity with Arg-Arg | Latilactobacillus sakei | Lys + Ala | - |
? | |
3.4.11.6 | Lys-Lys + H2O | 30% of the activity with Arg-Arg | Latilactobacillus sakei | Lys + Lys | - |
? | |
3.4.11.6 | additional information | no activity with Ala-Ala, Ala-Lys, Asp-Ala, Gly-Ala and Leu-Ala. The enzyme exclusively hydrolyzes basic amino acids from the amino termini of peptide substrates. The nature of the amino acid residue at the C-terminus of the dipeptide has an effect on hydrolysis rate. The activity is maximal towards dipeptides with Arg, Lys, or Ala as the C-terminal residue | Latilactobacillus sakei | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.11.6 | trimer | 3 * 60000, SDS-PAGE | Latilactobacillus sakei |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.11.6 | 37 | - |
- |
Latilactobacillus sakei |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.11.6 | 15 | 45 | 15°C: about 30% of maximal activity, 25°C: about 75% of maximal activity, 45°C: about 70% of maximal activity | Latilactobacillus sakei |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.11.6 | 5 | - |
- |
Latilactobacillus sakei |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.4.11.6 | 4.5 | 5.5 | pH 4.5: about 80% of maximal activity, pH 5.5: about 25% of maximal activity, activity is negligible at pH values beyond pH 4.0 and pH 6.0 | Latilactobacillus sakei |