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Literature summary extracted from

  • Wannet, W.J.B.; Wassenaar, R.W.; Jorissen, H.J.M.M.; Van der Drift, C.; Op Den Camp, H.J.M.
    Purification and characterization of an acid phosphatase from the commercial mushroom Agaricus bisporus (2000), Antonie van Leeuwenhoek, 77, 215-222.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.1.3.2 to minimize activity loss during the purification procedure the best stabilizing results are obtained by addition of a combination of 1 mM DTT, 1 mM EDTA and glycerol Agaricus bisporus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 additional information unaffected by EDTA Agaricus bisporus
3.1.3.2 phosphate
-
 Agaricus bisporus
3.1.3.2 Tartrate
-
 Agaricus bisporus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.2 0.0031
-
 D-fructose 6-phosphate pH 4.8, 30°C Agaricus bisporus
3.1.3.2 0.37
-
 4-nitrophenyl phosphate pH 4.8, 30°C Agaricus bisporus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.2 vacuole
-
 Agaricus bisporus 5773
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.2 39000
-
 4 * 39000, SDS-PAGE Agaricus bisporus
3.1.3.2 145000
-
 non-denaturing PAGE Agaricus bisporus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.2 additional information Agaricus bisporus key enzyme involved in the biosynthesis of mannitol ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Agaricus bisporus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.2 glycoprotein maximal carbohydrate content of 30% Agaricus bisporus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2
-
 Agaricus bisporus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.2 225
-
-
 Agaricus bisporus

Storage Stability

EC Number Storage Stability Organism
3.1.3.2 -20°C, enzyme in cell-free extract remains stable for 6 months Agaricus bisporus
3.1.3.2 4°C, enzyme in cell-free extract remains stable for at least 8 days Agaricus bisporus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 4-nitrophenyl phosphate + H2O
-
 Agaricus bisporus 4-nitrophenol + phosphate
-
 ?
3.1.3.2 AMP + H2O
-
 Agaricus bisporus adenosine + phosphate
-
 ?
3.1.3.2 beta-glycerol phosphate + H2O
-
 Agaricus bisporus glycerol + phosphate
-
 ?
3.1.3.2 D-fructose 6-phosphate + H2O
-
 Agaricus bisporus D-fructose + phosphate
-
 ?
3.1.3.2 D-glucose 6-phosphate + H2O
-
 Agaricus bisporus D-glucose + phosphate
-
 ?
3.1.3.2 D-mannitol 6-phosphate + H2O
-
 Agaricus bisporus D-mannitol + phosphate
-
 ?
3.1.3.2 D-ribose 5-phosphate + H2O
-
 Agaricus bisporus D-ribose + phosphate
-
 ?
3.1.3.2 additional information no activity is observed with fructose 1,6-diphopshate, glucose 1-phosphate, ATP, ADP and acetyl phosphate Agaricus bisporus ?
-
 ?
3.1.3.2 additional information key enzyme involved in the biosynthesis of mannitol Agaricus bisporus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.2 tetramer 4 * 39000, SDS-PAGE Agaricus bisporus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.2 65
-
 D-fructose 6-phosphate as substrate Agaricus bisporus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.2 40 70 40°C: about 60% of maximal activity, 70°C: about 70% of maximal activity, D-fructose 6-phosphate as substrate Agaricus bisporus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 4.8
-
 D-fructose 6-phosphate as substrate Agaricus bisporus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.2 4 5.5 pH 2.0: no activity, pH 4.0: about 90% of maximal activity, pH 5.5: about 60% of maximal activity, D-fructose 6-phosphate as substrate Agaricus bisporus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.3.2 5
-
 phosphate pH 4.8, 30°C Agaricus bisporus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.3.2 Agaricus bisporus isoelectric focusing 4.8 4.7