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Literature summary extracted from
- Electrochemiluminescence assay for basic carboxypeptidases: inhibition of basic carboxypeptidases and activation of thrombin-activatable fibrinolysis inhibitor (2003), Anal. Biochem., 319, 159-170.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.17.2 | 2-Guanidinoethylmercaptosuccinic acid | - |
Homo sapiens |  |
| 3.4.17.2 | DL-2-mercaptomethyl-3-guanidinoethylthiopropanoic acid | - |
Homo sapiens | |
| 3.4.17.2 | epsilon-aminocaproic acid | - |
Homo sapiens | |
| 3.4.17.2 | potato carboxypeptidase inhibitor | - |
Homo sapiens | |
| 3.4.17.2 | protamine | - |
Homo sapiens | |
| 3.4.17.20 | 2-Guanidinoethylmercaptosuccinic acid | - |
Homo sapiens | |
| 3.4.17.20 | DL-2-mercaptomethyl-3-guanidinoethylthiopropanoic acid | - |
Homo sapiens | |
| 3.4.17.20 | epsilon-aminocaproic acid | - |
Homo sapiens | |
| 3.4.17.20 | potato carboxypeptidase inhibitor | - |
Homo sapiens |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.17.2 | 0.008 | - |
biotin-(epsilon-aminocaproic acid)2-GLMVGGVVR | pH 7.4 | Homo sapiens | |
| 3.4.17.2 | 0.012 | - |
3-(2-furyl)acryloyl-L-Ala-L-Arg | - |
Homo sapiens | |
| 3.4.17.20 | 0.012 | - |
biotinyl-(epsilon-aminocaproic acid)-(epsilon-aminocaproic acid)-Gly-Leu-Met-Val-Gly-Gly-Val-Val-Arg-OH | room temperature | Homo sapiens | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.17.2 | Homo sapiens | - |
- |
- |
| 3.4.17.20 | Canis lupus familiaris | - |
- |
- |
| 3.4.17.20 | Chlorocebus aethiops | - |
- |
- |
| 3.4.17.20 | Homo sapiens | - |
- |
- |
| 3.4.17.20 | Macaca mulatta | - |
- |
- |
| 3.4.17.20 | Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.17.20 | development of a method to isolate in situ activated TAFIa from human serum in presence of epsilon-aminocaproic acid | Homo sapiens |
| 3.4.17.20 | development of a method to isolate in situ activated TAFIa from serum in presence of epsilon-aminocaproic acid | Rattus norvegicus |
| 3.4.17.20 | development of a method to isolate in situ activated TAFIa from serum in presence of epsilon-aminocaproic acid | Canis lupus familiaris |
| 3.4.17.20 | development of a method to isolate in situ activated TAFIa from serum in presence of epsilon-aminocaproic acid | Macaca mulatta |
| 3.4.17.20 | development of a method to isolate in situ activated TAFIa from serum in presence of epsilon-aminocaproic acid | Chlorocebus aethiops |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.17.20 | blood serum | - |
Homo sapiens | - |
| 3.4.17.20 | blood serum | - |
Rattus norvegicus | - |
| 3.4.17.20 | blood serum | - |
Canis lupus familiaris | - |
| 3.4.17.20 | blood serum | - |
Macaca mulatta | - |
| 3.4.17.20 | blood serum | - |
Chlorocebus aethiops | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.17.2 | additional information | - |
electrochemiluminescence assay. Development of a highly sensitive method for measurement of basic carboxypeptidase activity. The assay format depends on the recognition of a C-terminal carboxylic acid residue of a peptide with the C-terminal sequence GGVV by the monoclonal antibody, G2-10. Removal of the masking C-terminal Arg by a basic carboxypeptidase exposes the recognition site for the antibody. High sensitivity, detection limits of 1-2 pM | Homo sapiens |
| 3.4.17.20 | additional information | - |
electroluminescence assay. The high sensitivity of the assay allows the determination of the activatable levels of TAFI in human and other animal plasma in presence of epsilon-aminocaproic acid, an active-site inhibitor that stabilizes TAFIa | Homo sapiens |
| 3.4.17.20 | additional information | - |
electroluminescence assay. The high sensitivity of the assay allows the determination of the activatable levels of TAFI in human and other animal plasma in presence of epsilon-aminocaproic acid, an active-site inhibitor that stabilizes TAFIa | Rattus norvegicus |
| 3.4.17.20 | additional information | - |
electroluminescence assay. The high sensitivity of the assay allows the determination of the activatable levels of TAFI in human and other animal plasma in presence of epsilon-aminocaproic acid, an active-site inhibitor that stabilizes TAFIa | Canis lupus familiaris |
| 3.4.17.20 | additional information | - |
electroluminescence assay. The high sensitivity of the assay allows the determination of the activatable levels of TAFI in human and other animal plasma in presence of epsilon-aminocaproic acid, an active-site inhibitor that stabilizes TAFIa | Macaca mulatta |
| 3.4.17.20 | additional information | - |
electroluminescence assay. The high sensitivity of the assay allows the determination of the activatable levels of TAFI in human and other animal plasma in presence of epsilon-aminocaproic acid, an active-site inhibitor that stabilizes TAFIa | Chlorocebus aethiops |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.17.2 | 3-(2-furyl)acryloyl-L-Ala-L-Arg + H2O | - |
Homo sapiens | 3-(2-furyl)acryloyl-L-Ala + L-Arg | - |
? | |
| 3.4.17.2 | biotin-(epsilon-aminocaproic acid)2-GLMVGGVVR + H2O | - |
Homo sapiens | biotin-(epsilon-aminocaproic acid)2-GLMVGGVV + Arg | - |
? | |
| 3.4.17.20 | biotinyl-(epsilon-aminocaproic acid)-(epsilon-aminocaproic acid)-Gly-Leu-Met-Val-Gly-Gly-Val-Val-Arg-OH + H2O | - |
Homo sapiens | biotinyl-(epsilon-aminocaproic acid)-(epsilon-aminocaproic acid)-Gly-Leu-Met-Val-Gly-Gly-Val-Val-OH + Arg | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.17.2 | PCB | - |
Homo sapiens |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.17.2 | 1.9 | - |
biotin-(epsilon-aminocaproic acid)2-GLMVGGVVR | pH 7.4 | Homo sapiens | |
| 3.4.17.20 | 2.3 | - |
biotinyl-(epsilon-aminocaproic acid)-(epsilon-aminocaproic acid)-Gly-Leu-Met-Val-Gly-Gly-Val-Val-Arg-OH | room temperature | Homo sapiens | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.17.2 | 0.000001 | - |
potato carboxypeptidase inhibitor | pH 7.4 | Homo sapiens | |
| 3.4.17.2 | 0.0000082 | - |
DL-2-mercaptomethyl-3-guanidinoethylthiopropanoic acid | pH 7.4 | Homo sapiens | |
| 3.4.17.2 | 0.00005 | - |
2-Guanidinoethylmercaptosuccinic acid | pH 7.4 | Homo sapiens | |
| 3.4.17.2 | 0.01 | - |
protamine | above, pH 7.4 | Homo sapiens | |
| 3.4.17.2 | 0.9 | - |
epsilon-aminocaproic acid | pH 7.4 | Homo sapiens | |
| 3.4.17.20 | additional information | - |
additional information | the Ki-value for protamine is above 50 mM | Homo sapiens | |
| 3.4.17.20 | 0.0000021 | - |
potato carboxypeptidase inhibitor | - |
Homo sapiens | |
| 3.4.17.20 | 0.0002 | - |
DL-2-mercaptomethyl-3-guanidinoethylthiopropanoic acid | - |
Homo sapiens | |
| 3.4.17.20 | 0.0044 | - |
2-Guanidinoethylmercaptosuccinic acid | - |
Homo sapiens | |
| 3.4.17.20 | 1 | - |
epsilon-aminocaproic acid | - |
Homo sapiens | |
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