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Literature summary extracted from

  • Oliveira, V.; Campos, M.; Hemerly, J.P.; Ferro, E.S.; Camargo, A.C.; Juliano, M.A.; Juliano, L.
    Selective neurotensin-derived internally quenched fluorogenic substrates for neurolysin (EC 3.4.24.16): comparison with thimet oligopeptidase (EC 3.4.24.15) and neprilysin (EC 3.4.24.11) (2001), Anal. Biochem., 292, 257-265.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.15 0.0028
-
 (ortho-aminobenzoyl)-GGFLPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) pH 8.0, 37°C, hydrolysis of R-R bond Rattus norvegicus
3.4.24.15 0.007
-
 (ortho-aminobenzoyl)-LYENKPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) pH 8.0, 37°C, hydrolysis of R-R bond Rattus norvegicus
3.4.24.15 0.0071
-
 (o-aminobenzoyl)-NAPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) pH 8.0, 37°C, hydrolysis of R-R bond Rattus norvegicus
3.4.24.15 0.0102
-
 (o-aminobenzoyl)-AKPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) pH 8.0, 37°C, hydrolysis of R-R bond Rattus norvegicus
3.4.24.15 0.0121
-
 (ortho-aminobenzoyl)-NKPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) pH 8.0, 37°C, hydrolysis of R-R bond Rattus norvegicus
3.4.24.15 0.0124
-
 (o-aminobenzoyl)-NKPRRAQ-(N-(2,4-dinitrophenyl)ethylenediamine) pH 8.0, 37°C, hydrolysis of R-R bond Rattus norvegicus
3.4.24.15 0.015
-
 (o-aminobenzoyl)-NKPRAPQ-(N-(2,4-dinitrophenyl)ethylenediamine) pH 8.0, 37°C, hydrolysis of R-R bond Rattus norvegicus
3.4.24.15 0.021
-
 (ortho-aminobenzoyl)-ENKPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) pH 8.0, 37°C, hydrolysis of R-R bond Rattus norvegicus
3.4.24.15 0.036
-
 (o-aminobenzoyl)-NKPARPQ-(N-(2,4-dinitrophenyl)ethylenediamine) pH 8.0, 37°C, hydrolysis of R-R bond Rattus norvegicus
3.4.24.15 0.064
-
 (o-aminobenzoyl)-NKARRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) pH 8.0, 37°C, hydrolysis of R-R bond Rattus norvegicus
3.4.24.15 0.114
-
 (ortho-aminobenzoyl)-KPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) pH 8.0, 37°C, hydrolysis of R-R bond Rattus norvegicus
3.4.24.16 0.00025
-
 (o-aminobenzoyl)-NKPRRAQ-(N-(2,4-dinitrophenyl)ethylenediamine) pH 8.0, 37°C, hydrolysis of R-R bond Sus scrofa
3.4.24.16 0.00044
-
 (o-aminobenzoyl)-LYENKPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) pH 8.0, 37°C, hydrolysis of R-R bond Sus scrofa
3.4.24.16 0.00047
-
 (o-aminobenzoyl)-GGFLPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) pH 8.0, 37°C, hydrolysis of R-R bond Sus scrofa
3.4.24.16 0.00056
-
 (o-aminobenzoyl)-NKPARPQ-(N-(2,4-dinitrophenyl)ethylenediamine) pH 8.0, 37°C, hydrolysis of R-R bond Sus scrofa
3.4.24.16 0.00082
-
 (o-aminobenzoyl)-NKPRAPQ-(N-(2,4-dinitrophenyl)ethylenediamine) pH 8.0, 37°C, hydrolysis of R-R bond Sus scrofa
3.4.24.16 0.00103
-
 (o-aminobenzoyl)-NAPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) pH 8.0, 37°C, hydrolysis of R-R bond Sus scrofa
3.4.24.16 0.00115
-
 (o-aminobenzoyl)-AKPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) pH 8.0, 37°C, hydrolysis of R-R bond Sus scrofa
3.4.24.16 0.00119
-
 (o-aminobenzoyl)-NKPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) pH 8.0, 37°C, hydrolysis of R-R bond Sus scrofa
3.4.24.16 0.00138
-
 (o-aminobenzoyl)-NKARRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) pH 8.0, 37°C, hydrolysis of R-R bond Sus scrofa
3.4.24.16 0.0033
-
 (o-aminobenzoyl)-KPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) pH 8.0, 37°C, hydrolysis of R-R bond Sus scrofa
3.4.24.16 0.0058
-
 (o-aminobenzoyl)-ENKPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) pH 8.0, 37°C, hydrolysis of R-R bond Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.15 Rattus norvegicus
-
-
-
3.4.24.16 Sus scrofa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.15 testis
-
 Rattus norvegicus
-
3.4.24.16 kidney
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.15 (o-aminobenzoyl)-AKPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
-
 Rattus norvegicus (o-aminobenzoyl)-AKPR + RPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
 ?
3.4.24.15 (o-aminobenzoyl)-ENKPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
-
 Rattus norvegicus (o-aminobenzoyl)-ENKPR + RPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
 ?
3.4.24.15 (o-aminobenzoyl)-ENKPRRPYIQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O 84% cleavage at R-R bond, 16% cleavage at P-Y bond Rattus norvegicus (ortho-aminobenzoyl)-ENKPR + RPYIQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (ortho-aminobenzoyl)-KPRRP + YIQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
 ?
3.4.24.15 (o-aminobenzoyl)-ENKPRRPYQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O 16% cleavage at P-Y bond, 40% cleavage at Y-Q bond, 44% cleavage at R-R Rattus norvegicus (o-aminobenzoyl)-ENKPR + RPYQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
 ?
3.4.24.15 (o-aminobenzoyl)-GGFLPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
-
 Rattus norvegicus (o-aminobenzoyl)-GGFLPR + RPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
 ?
3.4.24.15 (o-aminobenzoyl)-KPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
-
 Rattus norvegicus (o-aminobenzoyl)-KPR + RPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
 ?
3.4.24.15 (o-aminobenzoyl)-LYENKPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
-
 Rattus norvegicus (o-aminobenzoyl)-LYENKPR + RPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
 ?
3.4.24.15 (o-aminobenzoyl)-LYENKPRRPYILQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O 68% cleavage at P-Y bond, 32% cleavage at Y-I bond Rattus norvegicus (o-aminobenzoyl)-LYENKPRRP + YILQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (ortho-aminobenzoyl)-LYENKPRRPY + ILQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
 ?
3.4.24.15 (o-aminobenzoyl)-NAPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
-
 Rattus norvegicus (o-aminobenzoyl)-NAPR + RPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
 ?
3.4.24.15 (o-aminobenzoyl)-NKARRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
-
 Rattus norvegicus (o-aminobenzoyl)-NKAR + RPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
 ?
3.4.24.15 (o-aminobenzoyl)-NKARRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
-
 Rattus norvegicus ?
-
 ?
3.4.24.15 (o-aminobenzoyl)-NKPARPQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
-
 Rattus norvegicus ?
-
 ?
3.4.24.15 (o-aminobenzoyl)-NKPRAPQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
-
 Rattus norvegicus ?
-
 ?
3.4.24.15 (o-aminobenzoyl)-NKPRRAPQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
-
 Rattus norvegicus ?
-
 ?
3.4.24.15 (o-aminobenzoyl)-NKPRRAQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
-
 Rattus norvegicus (o-aminobenzoyl)-NKPR + RAQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
 ?
3.4.24.15 (o-aminobenzoyl)-NKPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
-
 Rattus norvegicus (o-aminobenzoyl)-NKPR + RPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
 ?
3.4.24.15 additional information no activity with (ortho-aminobenzoyl)-PRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine), (ortho-aminobenzoyl)-KPRRPYILQ-(N-(2,4-dinitrophenyl)ethylenediamine) or (ortho-aminobenzoyl)-ENKPRRPYILQ-(N-(2,4-dinitrophenyl)ethylenediamine) Rattus norvegicus ?
-
 ?
3.4.24.16 (o-aminobenzoyl)-AKPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
-
 Sus scrofa (o-aminobenzoyl)-AKPR + RPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
 ?
3.4.24.16 (o-aminobenzoyl)-ENKPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
-
 Sus scrofa (o-aminobenzoyl)-ENKPR + RPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
 ?
3.4.24.16 (o-aminobenzoyl)-ENKPRRPYILQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O 36% cleavage at P-Y bond, 64% cleavage at Y-I bond Sus scrofa (o-aminobenzoyl)-ENKPRRP + YILQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-KPRRPY + ILQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
 ?
3.4.24.16 (o-aminobenzoyl)-ENKPRRPYIQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O 79% cleavage at P-Y bond, 21% cleavage at Y-I bond Sus scrofa (o-aminobenzoyl)-ENKPRRP + YIQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-KPRRPY + IQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
 ?
3.4.24.16 (o-aminobenzoyl)-ENKPRRPYQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O 16% cleavage at P-Y bond, 40% cleavage at Y-Q bond, 44% cleavage at R-R Sus scrofa (o-aminobenzoyl)-ENKPRRP + YQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-KPRRPY + Q-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-ENKPR + RPYQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
 ?
3.4.24.16 (o-aminobenzoyl)-GGFLPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
-
 Sus scrofa (o-aminobenzoyl)-GGFLPR + RPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
 ?
3.4.24.16 (o-aminobenzoyl)-KPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
-
 Sus scrofa (o-aminobenzoyl)-KPR + RPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
 ?
3.4.24.16 (o-aminobenzoyl)-KPRRPYILQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O 37% cleavage at P-Y bond, 63% cleavage at Y-I bond Sus scrofa (o-aminobenzoyl)-KPRRP + YILQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-KPRRPY + ILQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
 ?
3.4.24.16 (o-aminobenzoyl)-LYENKPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
-
 Sus scrofa (o-aminobenzoyl)-LYENKPR + RPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
 ?
3.4.24.16 (o-aminobenzoyl)-LYENKPRRPYILQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O 65% cleavage at P-Y bond, 35% cleavage at Y-I bond Sus scrofa (o-aminobenzoyl)-LYENKPRRP + YILQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-LYENKPRRPY + ILQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
 ?
3.4.24.16 (o-aminobenzoyl)-NAPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
-
 Sus scrofa (o-aminobenzoyl)-NAPR + RPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
 ?
3.4.24.16 (o-aminobenzoyl)-NKARRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
-
 Sus scrofa (o-aminobenzoyl)-NKAR + RPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
 ?
3.4.24.16 (o-aminobenzoyl)-NKPARPQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
-
 Sus scrofa ?
-
 ?
3.4.24.16 (o-aminobenzoyl)-NKPRAPQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
-
 Sus scrofa ?
-
 ?
3.4.24.16 (o-aminobenzoyl)-NKPRRAQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
-
 Sus scrofa (o-aminobenzoyl)-NKPR + RAQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
 ?
3.4.24.16 (o-aminobenzoyl)-NKPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
-
 Sus scrofa (o-aminobenzoyl)-NKPR + RPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
 ?
3.4.24.16 additional information no activity with (o-aminobenzoyl)-PRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) Sus scrofa ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.24.15 0.3
-
 (ortho-aminobenzoyl)-LYENKPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) pH 8.0, 37°C, hydrolysis of R-R bond Rattus norvegicus
3.4.24.15 0.7
-
 (ortho-aminobenzoyl)-NKPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) pH 8.0, 37°C, hydrolysis of R-R bond Rattus norvegicus
3.4.24.15 0.75
-
 (o-aminobenzoyl)-AKPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) pH 8.0, 37°C, hydrolysis of R-R bond Rattus norvegicus
3.4.24.15 3.2
-
 (ortho-aminobenzoyl)-ENKPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) pH 8.0, 37°C, hydrolysis of R-R bond Rattus norvegicus
3.4.24.15 3.6
-
 (o-aminobenzoyl)-NAPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) pH 8.0, 37°C, hydrolysis of R-R bond Rattus norvegicus
3.4.24.15 3.7
-
 (ortho-aminobenzoyl)-KPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) pH 8.0, 37°C, hydrolysis of R-R bond Rattus norvegicus
3.4.24.15 3.7
-
 (ortho-aminobenzoyl)-GGFLPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) pH 8.0, 37°C, hydrolysis of R-R bond Rattus norvegicus
3.4.24.15 4.2
-
 (o-aminobenzoyl)-NKPARPQ-(N-(2,4-dinitrophenyl)ethylenediamine) pH 8.0, 37°C, hydrolysis of R-R bond Rattus norvegicus
3.4.24.15 5.7
-
 (o-aminobenzoyl)-NKPRRAPQ-(N-(2,4-dinitrophenyl)ethylenediamine) pH 8.0, 37°C, hydrolysis of R-R bond Rattus norvegicus
3.4.24.15 8
-
 (o-aminobenzoyl)-NKARRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) pH 8.0, 37°C, hydrolysis of R-R bond Rattus norvegicus
3.4.24.15 8.7
-
 (o-aminobenzoyl)-NKPRAPQ-(N-(2,4-dinitrophenyl)ethylenediamine) pH 8.0, 37°C, hydrolysis of R-R bond Rattus norvegicus
3.4.24.16 0.00123
-
 (o-aminobenzoyl)-NKPARPQ-(N-(2,4-dinitrophenyl)ethylenediamine) pH 8.0, 37°C, hydrolysis of R-R bond Sus scrofa
3.4.24.16 0.00124
-
 (o-aminobenzoyl)-NKPRRAQ-(N-(2,4-dinitrophenyl)ethylenediamine) pH 8.0, 37°C, hydrolysis of R-R bond Sus scrofa
3.4.24.16 0.00497
-
 (o-aminobenzoyl)-AKPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) pH 8.0, 37°C, hydrolysis of R-R bond Sus scrofa
3.4.24.16 0.00576
-
 (o-aminobenzoyl)-NKARRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) pH 8.0, 37°C, hydrolysis of R-R bond Sus scrofa
3.4.24.16 0.00697
-
 (o-aminobenzoyl)-NAPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) pH 8.0, 37°C, hydrolysis of R-R bond Sus scrofa
3.4.24.16 0.014
-
 (o-aminobenzoyl)-NKPRAPQ-(N-(2,4-dinitrophenyl)ethylenediamine) pH 8.0, 37°C, hydrolysis of R-R bond Sus scrofa
3.4.24.16 1.35
-
 (o-aminobenzoyl)-ENKPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) pH 8.0, 37°C, hydrolysis of R-R bond Sus scrofa
3.4.24.16 1.77
-
 (o-aminobenzoyl)-GGFLPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) pH 8.0, 37°C, hydrolysis of R-R bond Sus scrofa
3.4.24.16 2.63
-
 (o-aminobenzoyl)-KPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) pH 8.0, 37°C, hydrolysis of R-R bond Sus scrofa
3.4.24.16 2.94
-
 (o-aminobenzoyl)-GGFLPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) pH 8.0, 37°C, hydrolysis of R-R bond Sus scrofa
3.4.24.16 3 6 (o-aminobenzoyl)-KPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) pH 8.0, 37°C, hydrolysis of R-R bond Sus scrofa
3.4.24.16 3.8
-
 (o-aminobenzoyl)-LYENKPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) pH 8.0, 37°C, hydrolysis of R-R bond Sus scrofa
3.4.24.16 7
-
 (o-aminobenzoyl)-NKPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) pH 8.0, 37°C, hydrolysis of R-R bond Sus scrofa