EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.24.B15 | DNA determination and analysis, expression of wild-type and C-terminally truncated mutant as His-tagged protein in Spodoptera frugiperda Sf9 cells via baculovirus infection | Mus musculus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.4.24.B15 | additional information | construction of C-terminally truncated enzyme version | Mus musculus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.4.24.B15 | membrane | - |
Homo sapiens | 16020 | - |
3.4.24.B15 | membrane | type II membrane-bound zinc metalloprotease | Mus musculus | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.B15 | Zinc | zinc metalloprotease | Homo sapiens | |
3.4.24.B15 | Zinc | type II membrane-bound zinc metalloprotease | Mus musculus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.24.B15 | 60000 | - |
x * 60000, recombinant C-terminally truncated mutant, SDS-PAGE, x * 86000, deglycosylated recombinant enzyme, SDS-PAGE, x * 100000, glycosylated recombinant enzyme, SDS-PAGE | Mus musculus |
3.4.24.B15 | 86000 | - |
x * 60000, recombinant C-terminally truncated mutant, SDS-PAGE, x * 86000, deglycosylated recombinant enzyme, SDS-PAGE, x * 100000, glycosylated recombinant enzyme, SDS-PAGE | Mus musculus |
3.4.24.B15 | 100000 | - |
x * 60000, recombinant C-terminally truncated mutant, SDS-PAGE, x * 86000, deglycosylated recombinant enzyme, SDS-PAGE, x * 100000, glycosylated recombinant enzyme, SDS-PAGE | Mus musculus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.B15 | protein + H2O | Mus musculus | - |
peptides | - |
? | |
3.4.24.B15 | protein + H2O | Homo sapiens | - |
peptides | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.24.B15 | Homo sapiens | - |
- |
- |
3.4.24.B15 | Mus musculus | - |
type II membrane-bound zinc metalloprotease | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.4.24.B15 | glycoprotein | 6 N-glycosylation sites, recombinant and wild-type enzyme | Mus musculus |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.24.B15 | recombinant from SF9 insect cells as His-tagged protein | Mus musculus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.4.24.B15 | proteolytic degradation of proteins | enzyme is an endopeptidase | Homo sapiens | |
3.4.24.B15 | proteolytic degradation of proteins | enzyme is an endopeptidase | Mus musculus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.24.B15 | MC3T3-E1 cell | osteoblast cell culture | Mus musculus | - |
3.4.24.B15 | MG-63 cell | osteoblast cell culture | Homo sapiens | - |
3.4.24.B15 | osteoblast | - |
Mus musculus | - |
3.4.24.B15 | osteoblast | cell culture | Homo sapiens | - |
3.4.24.B15 | SAOS-2 cell | osteoblast cell culture | Homo sapiens | - |
3.4.24.B15 | U2-OS cell | osteoblast cell culture | Homo sapiens | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.B15 | additional information | human stanniocalcin STC-1 is no substrate, recombinant rat parathyroid hormone is no substrate for the wild-type and mutant recombinant enzyme | Mus musculus | ? | - |
? | |
3.4.24.B15 | additional information | human stanniocalcin STC-1, casein and wild-type as well as mutant FGF-23 proteins are no substrates, recombinant rat parathyroid hormone is no substrate for the wild-type and mutant recombinant enzyme | Homo sapiens | ? | - |
? | |
3.4.24.B15 | protein + H2O | - |
Mus musculus | peptides | - |
? | |
3.4.24.B15 | protein + H2O | - |
Homo sapiens | peptides | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.24.B15 | ? | x * 60000, recombinant C-terminally truncated mutant, SDS-PAGE, x * 86000, deglycosylated recombinant enzyme, SDS-PAGE, x * 100000, glycosylated recombinant enzyme, SDS-PAGE | Mus musculus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.24.B15 | M13.091 | Merops-ID | Mus musculus |
3.4.24.B15 | M13.091 | Merops-ID | Homo sapiens |
3.4.24.B15 | PHEX | - |
Mus musculus |
3.4.24.B15 | PHEX | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.24.B15 | 37 | - |
assay at | Mus musculus |
3.4.24.B15 | 37 | - |
assay at | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.24.B15 | 7.4 | - |
assay at | Homo sapiens |
3.4.24.B15 | 7.4 | - |
assay at | Mus musculus |