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Literature summary extracted from

  • Murakami-Murofushi, K.; Takahashi, T.; Murofushi, H.; Takahashi, K.
    A novel intracellular acid proteinase from the plasmodia of a true slime mold, Physarum polycephalum (1995), Adv. Exp. Med. Biol., 362, 565-568.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.103 Diazoacetyl-DL-norleucine methyl ester 0.042 mg/ml, 95% inhibition Physarum polycephalum
3.4.21.103 PMSF 10 mM, 21% inhibition Physarum polycephalum
3.4.21.103 tosyl-L-Leu-chloromethylketone 10 mM, 18% inhibition Physarum polycephalum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.103 intracellular
-
 Physarum polycephalum 5622
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.103 23000
-
 1 * 31000 + 1 * 23000, the heavy and the light chain are cross-linked by disulfide bonds, SDS-PAGE Physarum polycephalum
3.4.21.103 31000
-
 1 * 31000 + 1 * 23000, the heavy and the light chain are cross-linked by disulfide bonds, SDS-PAGE Physarum polycephalum
3.4.21.103 54000
-
-
 Physarum polycephalum

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.103 Physarum polycephalum
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.21.103 glycoprotein the heavy chain, but not the light chain is glycosylated. One molecule of native enzyme contains about 7 residues of mannose, 5 residues of glucosamine, and 1 residue each of fucose and glucose Physarum polycephalum

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.103
-
 Physarum polycephalum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.103 plasmodium
-
 Physarum polycephalum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.103 Hemoglobin + H2O
-
 Physarum polycephalum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.103 dimer 1 * 31000 + 1 * 23000, the heavy and the light chain are cross-linked by disulfide bonds, SDS-PAGE Physarum polycephalum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.103 25
-
 pH 3.0-6.0, stable Physarum polycephalum
3.4.21.103 37
-
 pH 8.0, 15 min, 30% loss of activity Physarum polycephalum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.103 1.7
-
 hydrolysis of hemoglobin Physarum polycephalum