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Literature summary extracted from

  • Richards, N.G.J.; Schuster, S.M.
    Mechanistic issues in asparagine synthetase catalysis (1998), Adv. Enzymol. Relat. Areas Mol. Biol., 72, 145-198.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
6.3.5.4 E317A diminished glutaminase activity Escherichia coli
6.3.5.4 R322Q 60000fold decrease in kcat/KM for ATP-diphsphate exchange Escherichia coli
6.3.5.4 R325A no asparagine synthetase activity, glutaminase activity is retained Escherichia coli
6.3.5.4 R325K no asparagine synthetase activity, glutaminase activity is retained Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.5.4 beta-methylaspartate weak inhibitor Escherichia coli
6.3.5.4 pyrrolidine-2,3-dicarboxylic acid weak inhibitor Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.5.4 0.18
-
 ATP glutamine dependent asparagine synthetase activity Escherichia coli
6.3.5.4 0.68
-
 L-aspartate glutamine dependent asparagine synthetase activity Escherichia coli
6.3.5.4 0.69
-
 L-glutamine glutamine dependent asparagine synthetase activity Escherichia coli
6.3.5.4 1.39
-
 L-glutamine glutaminase activity in the presence of ATP Escherichia coli
6.3.5.4 1.9
-
 L-glutamine glutaminase activity in the absence of ATP Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.5.4 ATP + L-aspartate + L-glutamine Escherichia coli
-
 AMP + diphosphate + L-asparagine + L-glutamate
-
 ?
6.3.5.4 ATP + L-aspartate + NH3 Escherichia coli asparagine biosynthesis proceeds by initial reaction of aspartate and ATP to yield a beta-aspartyl-AMP intermediate, in the presence of glutamine, ammonia released in the N-terminal active site reacts with beta-aspartyl-AMP to yield asparagine and AMP AMP + diphosphate + L-asparagine
-
 ?
6.3.5.4 L-glutamine Escherichia coli
-
 L-glutamate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.4 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.4 ATP + L-aspartate + L-glutamine
-
 Escherichia coli AMP + diphosphate + L-asparagine + L-glutamate
-
 ?
6.3.5.4 ATP + L-aspartate + NH3 asparagine biosynthesis proceeds by initial reaction of aspartate and ATP to yield a beta-aspartyl-AMP intermediate, in the presence of glutamine, ammonia released in the N-terminal active site reacts with beta-aspartyl-AMP to yield asparagine and AMP Escherichia coli AMP + diphosphate + L-asparagine
-
 ?
6.3.5.4 L-glutamine
-
 Escherichia coli L-glutamate + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.5.4 AS-B
-
 Escherichia coli
6.3.5.4 Asparagine synthetase B
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.5.4 0.8
-
 L-glutamine glutaminase activity in the absence of ATP Escherichia coli
6.3.5.4 1.01
-
 L-glutamine glutamine dependent asparagine synthetase activity Escherichia coli
6.3.5.4 1.05
-
 L-aspartate glutamine dependent asparagine synthetase activity Escherichia coli
6.3.5.4 1.1
-
 ATP glutamine dependent asparagine synthetase activity Escherichia coli
6.3.5.4 1.38
-
 L-glutamine glutaminase activity in the presence of ATP Escherichia coli
6.3.5.4 6.08
-
 L-glutamine glutaminase activity in the presence of ATP Escherichia coli
6.3.5.4 6.08
-
 L-glutamine glutamine dependent asparagine synthetase activity Escherichia coli