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Literature summary extracted from

  • Zhu, D.W.; Lorber, B.; Sauter, C.; Ng, J.D.; Benas, P.; Le Grimellec, C.; Giege, R.
    Growth kinetics, diffraction properties and effect of agarose on the stability of a novel crystal form of Thermus thermophilus aspartyl-tRNA synthetase-1 (2001), Acta Crystallogr. Sect. D, 57, 552-558.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.12 overexpression in Escherichia coli Thermus thermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.1.1.12 purified recombinant enzyme, hanging-drop vapour diffusion method, in presence of PEG 8000, at 293K, growth kinetics and solubility measurements, X-ray diffraction structure determination at 3.1 A resolution Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.12 ATP + L-aspartate + tRNAAsp Thermus thermophilus
-
 AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.12 Thermus thermophilus
-
 isozyme AspRS1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.12 recombinant from overexpressing Escherichia coli Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.12 ATP + L-aspartate + tRNAAsp
-
 Thermus thermophilus AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.12 Aspartic acid translase
-
 Thermus thermophilus
6.1.1.12 Aspartyl ribonucleate synthetase
-
 Thermus thermophilus
6.1.1.12 aspartyl ribonuleic synthetase
-
 Thermus thermophilus
6.1.1.12 Aspartyl-transfer ribonucleic acid synthetase
-
 Thermus thermophilus
6.1.1.12 Aspartyl-transfer RNA synthetase
-
 Thermus thermophilus
6.1.1.12 Aspartyl-tRNA synthetase
-
 Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.1.1.12 additional information
-
 thermostable isozyme AspRS1 Thermus thermophilus