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Literature summary extracted from
- Binding site characterization of polyenoic fatty-acid isomerase from the marine alga Ptilota filicina (1997), Biochemistry, 36, 2985-2992.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.3.13 | additional information | - |
additional information | study of the effect of the pH on kinetic parameters with several substrates | Ptilota filicina | |
| 5.3.3.13 | 0.0019 | - |
(4Z,7Z,10Z,13Z,16Z,19Z)-docosahexenoate | pH 7, 22°C | Ptilota filicina |  |
| 5.3.3.13 | 0.0096 | - |
(5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate | pH 7, 22°C | Ptilota filicina | |
| 5.3.3.13 | 0.0152 | - |
(5Z,8Z,11Z,14Z)-eicosatetraenoyl-N-ethanolamide | pH 9, 22°C | Ptilota filicina | |
| 5.3.3.13 | 0.017 | - |
(7Z,10Z,13Z,16Z)-docosatetraenoate | pH 7, 22°C | Ptilota filicina | |
| 5.3.3.13 | 0.0175 | - |
(5Z,8Z,11Z,14Z)-eicosatetraenoyl-N-ethanolamide | pH 7, 22°C | Ptilota filicina | |
| 5.3.3.13 | 0.0255 | - |
(5Z,8Z,11Z,14Z)-eicosatetraenoate | pH 7, 22°C | Ptilota filicina | |
| 5.3.3.13 | 0.0528 | - |
(5Z,8Z,11Z)-eicosatrienoate | pH 7, 22°C | Ptilota filicina | |
| 5.3.3.13 | 0.15 | - |
(6Z,9Z,12Z)-octadecatrienoate | - |
Ptilota filicina | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.3.3.13 | 61000 | - |
x * 61000, SDS-PAGE | Ptilota filicina |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.3.3.13 | Ptilota filicina | - |
- |
- |
| 5.3.3.13 | Ptilota filicina | - |
Oregon red marine alga | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.3.3.13 | - |
Ptilota filicina |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 5.3.3.13 | (5Z,8Z,11Z,14Z,17Z)-icosapentaenoate = (5Z,7E,9E,14Z,17Z)-icosapentaenoate | mechansim of hydrogen transfers in the isomerization reaction including a diene intermediate | Ptilota filicina |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.3.3.13 | additional information | - |
- |
Ptilota filicina |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 5.3.3.13 | -20°C, purified PFI, months, stable | Ptilota filicina |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.3.13 | (4Z,7Z,10Z,13Z,16Z,19Z)-docosahexenoate | excellent substrate | Ptilota filicina | (4Z,7Z,9E,11E,16Z,19Z)-docosahexenoate | - |
? | |
| 5.3.3.13 | (5Z,8Z,11Z)-eicosatrienoate | - |
Ptilota filicina | ? | - |
? | |
| 5.3.3.13 | (5Z,8Z,11Z,14Z)-eicosatetraenoate | arachidonic acid | Ptilota filicina | (5Z,7E,9E,14Z)-eicosatetraenoate | - |
? | |
| 5.3.3.13 | (5Z,8Z,11Z,14Z)-eicosatetraenoyl-N-ethanolamide | anandamide | Ptilota filicina | (5Z,7E,9E,14Z)-eicosatetraenoyl-N-ethanolamide | - |
? | |
| 5.3.3.13 | (5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate | - |
Ptilota filicina | (5Z,7E,9E,14Z,17Z)-eicosapentaenoate | - |
? | |
| 5.3.3.13 | (6Z,9Z,12Z)-octadecatrienoate | gamma-linolenic acid | Ptilota filicina | (6Z,8E,10E)-octadecatrienoate | - |
? | |
| 5.3.3.13 | (7Z,10Z,13Z,16Z)-docosatetraenoate | adrenic acid | Ptilota filicina | ? | - |
? | |
| 5.3.3.13 | (8Z,11Z,14Z)-eicosatrienoate | dihomo-gamma-linolenic acid, formation of two products: a conjugated triene and a conjugated diene | Ptilota filicina | ? | - |
? | |
| 5.3.3.13 | (9Z,12Z,15Z)-octadecatrienoate | alpha-linolenic acid | Ptilota filicina | ? | - |
? | |
| 5.3.3.13 | additional information | substrate binding site, PFI preferentially orients the substrate in the catalytic site with respect to the methyl terminus and likely reacts, preferentially, with the protonated form of the substrate, not: linoleic acid | Ptilota filicina | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.3.3.13 | ? | x * 61000, SDS-PAGE | Ptilota filicina |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.3.13 | 22 | - |
assay at | Ptilota filicina |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.3.13 | 11.6 | - |
(5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate | assuming one catalytic site per enzyme molecule | Ptilota filicina | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.3.13 | 6 | - |
below | Ptilota filicina |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.3.13 | additional information | - |
pH-dependence profile of PFI, catalytically active over a wide pH-range with (5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate as substrate | Ptilota filicina |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.3.13 | 4 | 10 | PFI is relatively stable at these pH extremes | Ptilota filicina |
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