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Literature summary extracted from

  • Fukui, T.; Ito, M.; Tomita, K.
    Purification and characterization of acetoacetyl-CoA synthetase from Zoogloea ramigera 1-16-M (1982), Eur. J. Biochem., 127, 423-428.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.2.1.16 in presence of 10% glycerol the enzyme can be frozen and thawed ten times without loss of activity Zoogloea ramigera

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.16 0.033
-
 ATP
-
 Zoogloea ramigera
6.2.1.16 0.076
-
 acetoacetate
-
 Zoogloea ramigera
6.2.1.16 0.091
-
 CoA
-
 Zoogloea ramigera
6.2.1.16 1.4
-
 L(+)-3-hydroxybutyrate
-
 Zoogloea ramigera

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.16 Ca2+ can replace Mg2+ in activation, 26% of the activation relative to Mg2+ Zoogloea ramigera
6.2.1.16 Cs+ can replace K+ in activation Zoogloea ramigera
6.2.1.16 K+ monovalent cation required Zoogloea ramigera
6.2.1.16 Mg2+
-
 Zoogloea ramigera
6.2.1.16 Mn2+ can replace Mg2+ in activation Zoogloea ramigera
6.2.1.16 Mn2+ 96% of the activation relative to Mg2+ Zoogloea ramigera
6.2.1.16 additional information absolute requirement for a monovalent cation and a divalent cation Zoogloea ramigera
6.2.1.16 NH4+ can replace K+ in activation Zoogloea ramigera
6.2.1.16 Ni2+ can replace Mg2+ in activation Zoogloea ramigera
6.2.1.16 Ni2+ 21% of the activation relative to Mg2+ Zoogloea ramigera
6.2.1.16 Rb+ can replace K+ in activation Zoogloea ramigera

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.16 72000
-
 gel filtration Zoogloea ramigera

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.16 Zoogloea ramigera
-
-
-
6.2.1.16 Zoogloea ramigera 1-16-M / ATCC 19623
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.16
-
 Zoogloea ramigera

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.16 52.2
-
-
 Zoogloea ramigera

Storage Stability

EC Number Storage Stability Organism
6.2.1.16 -20°C, 0.07 mg/ml enzyme, 10 mM Tris/HCl buffer, pH 7.5, 50% glycerol, 10 mM 2-mercaptoethanol, stable for at least 1 month Zoogloea ramigera

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.16 ATP + acetoacetate + CoA r Zoogloea ramigera AMP + diphosphate + acetoacetyl-CoA
-
 ?
6.2.1.16 ATP + acetoacetate + CoA no activity with GTP, UTP, CTP or ADP Zoogloea ramigera AMP + diphosphate + acetoacetyl-CoA
-
 ?
6.2.1.16 ATP + acetoacetate + CoA r Zoogloea ramigera 1-16-M / ATCC 19623 AMP + diphosphate + acetoacetyl-CoA
-
 ?
6.2.1.16 ATP + acetoacetate + CoA no activity with GTP, UTP, CTP or ADP Zoogloea ramigera 1-16-M / ATCC 19623 AMP + diphosphate + acetoacetyl-CoA
-
 ?
6.2.1.16 ATP + L-3-hydroxybutanoate + CoA about 15% of the activity relative to ATP Zoogloea ramigera AMP + diphosphate + L-3-hydroxybutyryl-CoA
-
 ?
6.2.1.16 ATP + L-3-hydroxybutanoate + CoA about 15% of the activity relative to ATP Zoogloea ramigera 1-16-M / ATCC 19623 AMP + diphosphate + L-3-hydroxybutyryl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.16 monomer 1 * 72000, SDS-PAGE Zoogloea ramigera

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.2.1.16
-
-
 1 d, 30% loss of activity, in absence of glycerol Zoogloea ramigera
6.2.1.16 30
-
 1 d, 70% loss of activity, in absence of glycerol Zoogloea ramigera

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.16 7
-
 formation of acetoacetate Zoogloea ramigera
6.2.1.16 8.4
-
 activation of acetoacetate Zoogloea ramigera