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Literature summary extracted from

  • Martinez-Blanco, H.; Reglero, A.; Rodriguez-Aparicio, L.B.; Luengo, J.M.
    Purification and biochemical characterization of phenylacetyl-CoA ligase from Pseudomonas putida (1990), J. Biol. Chem., 265, 7084-7090.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.2.1.30 purified enzyme is sensitive to repeated freezing Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.30 5,5'-dithiobis(2-nitrobenzoate) 1 mM, 92% inhibition Pseudomonas putida
6.2.1.30 Cu2+ 1 mM, 97% inhibition Pseudomonas putida
6.2.1.30 Decanoic acid
-
 Pseudomonas putida
6.2.1.30 Hg2+ 1 mM, 95% inhibition Pseudomonas putida
6.2.1.30 n-octylglucoside
-
 Pseudomonas putida
6.2.1.30 NEM 1 mM, 99% inhibition Pseudomonas putida
6.2.1.30 Nonanoic acid
-
 Pseudomonas putida
6.2.1.30 Nonidet P-40
-
 Pseudomonas putida
6.2.1.30 octanoic acid
-
 Pseudomonas putida
6.2.1.30 p-chloromercuribenzoate 1 mM, 96% inhibition Pseudomonas putida
6.2.1.30 SDS
-
 Pseudomonas putida
6.2.1.30 Tween 100
-
 Pseudomonas putida
6.2.1.30 Zn2+ 1 mM, 95% inhibition Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.30 1
-
 CoA pH 8.2, 30°C Pseudomonas putida
6.2.1.30 9.7
-
 ATP pH 8.2, 30°C Pseudomonas putida
6.2.1.30 16.5
-
 phenylacetate pH 8.2, 30°C Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.30 Mg2+ required Pseudomonas putida
6.2.1.30 Mn2+ 50% of the activation with Mg2+ Pseudomonas putida
6.2.1.30 additional information enzyme does not contain metal ions Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.30 48000
-
 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.30 ATP + phenylacetate + CoA Pseudomonas putida enzyme is involved in the catabolism of phenylacetic acid ?
-
 ?
6.2.1.30 ATP + phenylacetate + CoA Pseudomonas putida specifically induced by phenylacetic acid ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.30 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.30
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.30 additional information
-
-
 Pseudomonas putida
6.2.1.30 21
-
-
 Pseudomonas putida

Storage Stability

EC Number Storage Stability Organism
6.2.1.30 -20°C, crude enzyme is stable in presence of glycerol for 2-3 weeks, more than 50% loss of activity of the purified enzyme Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.30 ADP + phenylacetate + CoA 28% of the activity with ATP Pseudomonas putida ?
-
 ?
6.2.1.30 ATP + acetate + CoA 85% of the activity with phenylacetate Pseudomonas putida AMP + diphosphate + acetyl-CoA
-
 ?
6.2.1.30 ATP + butanoate + CoA 19% of the activity with phenylacetate Pseudomonas putida AMP + diphosphate + butanoyl-CoA
-
 ?
6.2.1.30 ATP + phenylacetate + CoA
-
 Pseudomonas putida AMP + diphosphate + phenylacetyl-CoA
-
 ?
6.2.1.30 ATP + phenylacetate + CoA enzyme is involved in the catabolism of phenylacetic acid Pseudomonas putida ?
-
 ?
6.2.1.30 ATP + phenylacetate + CoA specifically induced by phenylacetic acid Pseudomonas putida ?
-
 ?
6.2.1.30 ATP + propanoate + CoA 47% of the activity with phenylacetate Pseudomonas putida AMP + diphosphate + propanoyl-CoA
-
 ?
6.2.1.30 CTP + phenylacetate + CoA 2.8% of the activity with ATP Pseudomonas putida CMP + diphosphate + phenylacetyl-CoA
-
 ?
6.2.1.30 additional information no activity with: 3-hydroxyphenylacetic acid, 4-hydroxyphenylacetic acid, 3,4-dihydroxyphenylacetic acid Pseudomonas putida ?
-
 ?
6.2.1.30 UTP + phenylacetate + CoA 2.5% of the activity with ATP Pseudomonas putida UMP + diphosphate + phenylacetyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.30 monomer 1 * 48000, SDS-PAGE Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.2.1.30 30
-
-
 Pseudomonas putida

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.2.1.30 additional information
-
 glycerol, 20% v/v, ATP, MgCl2 or phenylacetic acid protects against thermal inactivation Pseudomonas putida
6.2.1.30 20
-
 45 min stable, about 15% loss of activity after 60 min Pseudomonas putida
6.2.1.30 25
-
 30 min, about 20% loss of activity Pseudomonas putida
6.2.1.30 30
-
 60 min, about 40% loss of activity Pseudomonas putida
6.2.1.30 37
-
 60 min, about 75% loss of activity Pseudomonas putida
6.2.1.30 45
-
 15 min, about 90% loss of activity Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.30 7 8.5
-
 Pseudomonas putida

pI Value

EC Number Organism Comment pI Value Maximum pI Value
6.2.1.30 Pseudomonas putida isoelectric focusing
-
 5.4