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Literature summary extracted from

  • Biegert, T.; Altenschmidt, U.; Eckerskorn, C.; Fuchs, G.
    Enzymes of anarobic metabolism pf phenolic compounds. 4-Hydroxybenzoate-CoA ligase from a denitrifying Pseudomonas species (1993), Eur. J. Biochem., 213, 555-561.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.27 2,4-Dihydroxybenzoate strong Pseudomonas sp.
6.2.1.27 2-hydroxy-4-methylbenzoate strong Pseudomonas sp.
6.2.1.27 5,5'-dithiobis(2-nitrobenzoate)
-
Pseudomonas sp.
6.2.1.27 PCMB
-
Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.27 0.037
-
4-hydroxybenzoate pH 7.8, 37°C Pseudomonas sp.
6.2.1.27 0.077
-
ATP pH 7.8, 37°C Pseudomonas sp.
6.2.1.27 0.125
-
coenzyme A pH 7.8, 37°C Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.27 48000
-
gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.27 ATP + 4-hydroxybenzoate + CoA Pseudomonas sp. initial step of anaerobic 4-hydroxybenzoate degradation AMP + diphosphate + 4-hydroxybenzoyl-CoA
-
?
6.2.1.27 ATP + 4-hydroxybenzoate + CoA Pseudomonas sp. K172 initial step of anaerobic 4-hydroxybenzoate degradation AMP + diphosphate + 4-hydroxybenzoyl-CoA
-
?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.27 Pseudomonas sp.
-
-
-
6.2.1.27 Pseudomonas sp. K172
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
6.2.1.27 oxygen-sensitive during initial stages of purification, can be stabilized by adding DTT and 10% glycerol Pseudomonas sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.27 strain K172 Pseudomonas sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.27 culture condition:4-hydroxybenzoate-grown cell in the mid-exponential growth phase Pseudomonas sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.27 37.3
-
-
Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.27 ATP + 4-aminobenzoate + CoA 30% of the activity with 4-hydroxybenzoate Pseudomonas sp. AMP + 4-aminobenzoyl-CoA + diphosphate
-
?
6.2.1.27 ATP + 4-fluorobenzoate + CoA 3.8% of the activity with 4-hydroxybenzoate Pseudomonas sp. AMP + 4-fluorobenzoyl-CoA + diphosphate
-
?
6.2.1.27 ATP + 4-hydroxybenzoate + CoA highly specific Pseudomonas sp. AMP + diphosphate + 4-hydroxybenzoyl-CoA
-
?
6.2.1.27 ATP + 4-hydroxybenzoate + CoA anaerob Pseudomonas sp. AMP + diphosphate + 4-hydroxybenzoyl-CoA
-
?
6.2.1.27 ATP + 4-hydroxybenzoate + CoA initial step of anaerobic 4-hydroxybenzoate degradation Pseudomonas sp. AMP + diphosphate + 4-hydroxybenzoyl-CoA
-
?
6.2.1.27 ATP + 4-hydroxybenzoate + CoA highly specific Pseudomonas sp. K172 AMP + diphosphate + 4-hydroxybenzoyl-CoA
-
?
6.2.1.27 ATP + 4-hydroxybenzoate + CoA anaerob Pseudomonas sp. K172 AMP + diphosphate + 4-hydroxybenzoyl-CoA
-
?
6.2.1.27 ATP + 4-hydroxybenzoate + CoA initial step of anaerobic 4-hydroxybenzoate degradation Pseudomonas sp. K172 AMP + diphosphate + 4-hydroxybenzoyl-CoA
-
?
6.2.1.27 ATP + phenylacetate + CoA 4.5% of the activity with 4-hydroxybenzoate Pseudomonas sp. AMP + phenylacetyl-CoA + diphosphate
-
?
6.2.1.27 CTP + 4-hydroxybenzoate + CoA 60% of the activity with ATP Pseudomonas sp. CMP + 4-hydroxybenzoyl-CoA + diphosphate
-
?
6.2.1.27 CTP + 4-hydroxybenzoate + CoA 60% of the activity with ATP Pseudomonas sp. K172 CMP + 4-hydroxybenzoyl-CoA + diphosphate
-
?
6.2.1.27 additional information not: benzoate, 3- or 2-hydroxybenzoate Pseudomonas sp. ?
-
?
6.2.1.27 additional information not: benzoate, 3- or 2-hydroxybenzoate Pseudomonas sp. K172 ?
-
?

Subunits

EC Number Subunits Comment Organism
6.2.1.27 monomer 1 * 48000, SDS-PAGE Pseudomonas sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.2.1.27 30
-
4-hydroxybenzoate pH 7.8, 37°C Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.27 8.2 8.5
-
Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.2.1.27 7 9.5 50% of activity maximum at pH 7.0 and 9.5, almost no activity at pH 6.0 and 10.0 Pseudomonas sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.2.1.27 0.0012
-
2,4-Dihydroxybenzoate pH 7.8, 37°C Pseudomonas sp.
6.2.1.27 0.0014
-
2-hydroxy-4-methylbenzoate pH 7.8, 37°C Pseudomonas sp.