EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.2.1.27 | 2,4-Dihydroxybenzoate | strong | Pseudomonas sp. | |
6.2.1.27 | 2-hydroxy-4-methylbenzoate | strong | Pseudomonas sp. | |
6.2.1.27 | 5,5'-dithiobis(2-nitrobenzoate) | - |
Pseudomonas sp. | |
6.2.1.27 | PCMB | - |
Pseudomonas sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.2.1.27 | 0.037 | - |
4-hydroxybenzoate | pH 7.8, 37°C | Pseudomonas sp. | |
6.2.1.27 | 0.077 | - |
ATP | pH 7.8, 37°C | Pseudomonas sp. | |
6.2.1.27 | 0.125 | - |
coenzyme A | pH 7.8, 37°C | Pseudomonas sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.2.1.27 | 48000 | - |
gel filtration | Pseudomonas sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.2.1.27 | ATP + 4-hydroxybenzoate + CoA | Pseudomonas sp. | initial step of anaerobic 4-hydroxybenzoate degradation | AMP + diphosphate + 4-hydroxybenzoyl-CoA | - |
? | |
6.2.1.27 | ATP + 4-hydroxybenzoate + CoA | Pseudomonas sp. K172 | initial step of anaerobic 4-hydroxybenzoate degradation | AMP + diphosphate + 4-hydroxybenzoyl-CoA | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.2.1.27 | Pseudomonas sp. | - |
- |
- |
6.2.1.27 | Pseudomonas sp. K172 | - |
- |
- |
EC Number | Oxidation Stability | Organism |
---|---|---|
6.2.1.27 | oxygen-sensitive during initial stages of purification, can be stabilized by adding DTT and 10% glycerol | Pseudomonas sp. |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.2.1.27 | strain K172 | Pseudomonas sp. |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
6.2.1.27 | culture condition:4-hydroxybenzoate-grown cell | in the mid-exponential growth phase | Pseudomonas sp. | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
6.2.1.27 | 37.3 | - |
- |
Pseudomonas sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.2.1.27 | ATP + 4-aminobenzoate + CoA | 30% of the activity with 4-hydroxybenzoate | Pseudomonas sp. | AMP + 4-aminobenzoyl-CoA + diphosphate | - |
? | |
6.2.1.27 | ATP + 4-fluorobenzoate + CoA | 3.8% of the activity with 4-hydroxybenzoate | Pseudomonas sp. | AMP + 4-fluorobenzoyl-CoA + diphosphate | - |
? | |
6.2.1.27 | ATP + 4-hydroxybenzoate + CoA | highly specific | Pseudomonas sp. | AMP + diphosphate + 4-hydroxybenzoyl-CoA | - |
? | |
6.2.1.27 | ATP + 4-hydroxybenzoate + CoA | anaerob | Pseudomonas sp. | AMP + diphosphate + 4-hydroxybenzoyl-CoA | - |
? | |
6.2.1.27 | ATP + 4-hydroxybenzoate + CoA | initial step of anaerobic 4-hydroxybenzoate degradation | Pseudomonas sp. | AMP + diphosphate + 4-hydroxybenzoyl-CoA | - |
? | |
6.2.1.27 | ATP + 4-hydroxybenzoate + CoA | highly specific | Pseudomonas sp. K172 | AMP + diphosphate + 4-hydroxybenzoyl-CoA | - |
? | |
6.2.1.27 | ATP + 4-hydroxybenzoate + CoA | anaerob | Pseudomonas sp. K172 | AMP + diphosphate + 4-hydroxybenzoyl-CoA | - |
? | |
6.2.1.27 | ATP + 4-hydroxybenzoate + CoA | initial step of anaerobic 4-hydroxybenzoate degradation | Pseudomonas sp. K172 | AMP + diphosphate + 4-hydroxybenzoyl-CoA | - |
? | |
6.2.1.27 | ATP + phenylacetate + CoA | 4.5% of the activity with 4-hydroxybenzoate | Pseudomonas sp. | AMP + phenylacetyl-CoA + diphosphate | - |
? | |
6.2.1.27 | CTP + 4-hydroxybenzoate + CoA | 60% of the activity with ATP | Pseudomonas sp. | CMP + 4-hydroxybenzoyl-CoA + diphosphate | - |
? | |
6.2.1.27 | CTP + 4-hydroxybenzoate + CoA | 60% of the activity with ATP | Pseudomonas sp. K172 | CMP + 4-hydroxybenzoyl-CoA + diphosphate | - |
? | |
6.2.1.27 | additional information | not: benzoate, 3- or 2-hydroxybenzoate | Pseudomonas sp. | ? | - |
? | |
6.2.1.27 | additional information | not: benzoate, 3- or 2-hydroxybenzoate | Pseudomonas sp. K172 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.2.1.27 | monomer | 1 * 48000, SDS-PAGE | Pseudomonas sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.2.1.27 | 30 | - |
4-hydroxybenzoate | pH 7.8, 37°C | Pseudomonas sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.2.1.27 | 8.2 | 8.5 | - |
Pseudomonas sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
6.2.1.27 | 7 | 9.5 | 50% of activity maximum at pH 7.0 and 9.5, almost no activity at pH 6.0 and 10.0 | Pseudomonas sp. |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.2.1.27 | 0.0012 | - |
2,4-Dihydroxybenzoate | pH 7.8, 37°C | Pseudomonas sp. | |
6.2.1.27 | 0.0014 | - |
2-hydroxy-4-methylbenzoate | pH 7.8, 37°C | Pseudomonas sp. |