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Literature summary extracted from

  • Kumagai, H.; Suzuki, H.; Shigematsu, H.; Tochikura, T.
    S-Carboxymethylcysteine synthase from Escherichia coli (1989), Agric. Biol. Chem., 53, 2481-2487.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.5.1.5 fine yellow needles Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.5.1.5 additional information not inhibited by the substrate 3-chloro-L-alanine Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.5.1.5 15.4
-
 thioglycolate pH 7.8, 37°C Escherichia coli
4.5.1.5 40
-
 3-chloro-L-alanine pH 7.8, 37°C Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.5.1.5 37000
-
 2 * 37000, SDS-PAGE Escherichia coli
4.5.1.5 42000
-
 2 * 42000, HPLC gel filtration in presence of SDS Escherichia coli
4.5.1.5 84000
-
 HPLC gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.5.1.5 additional information Escherichia coli constitutive enzyme ?
-
 ?
4.5.1.5 additional information Escherichia coli W 3110 constitutive enzyme ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.5.1.5 Escherichia coli
-
-
-
4.5.1.5 Escherichia coli W 3110
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.5.1.5 190fold Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.5.1.5 additional information
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
4.5.1.5 long-term storage of enzyme as ammonium sulfate precipitate decreases activity, reactivation by dialysis against 0.1 M potassium phosphate buffer, pH 7.8, containing 10 mM 2-mercaptoethanol, 0.02 mM pyridoxal phosphate, 4 mM EDTA Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.5.1.5 3-chloro-L-alanine + 1-thioglycerol 93% of activity compared to thioglycolate Escherichia coli S-2,3-dihydroxypropyl-L-cysteine + Cl-
-
 ?
4.5.1.5 3-chloro-L-alanine + 2-mercaptoethanol 242% of activity compared to thioglycolate Escherichia coli S-2-hydroxyethyl-L-cysteine + Cl-
-
 ?
4.5.1.5 3-chloro-L-alanine + benzylmercaptan 35% of activity compared to thioglycolate Escherichia coli S-benzyl-L-cysteine + Cl-
-
 ?
4.5.1.5 3-chloro-L-alanine + ethylmercaptan 55% of activity compared to thioglycolate Escherichia coli S-ethyl-L-cysteine + Cl-
-
 ?
4.5.1.5 3-chloro-L-alanine + ethylmercaptan 55% of activity compared to thioglycolate Escherichia coli W 3110 S-ethyl-L-cysteine + Cl-
-
 ?
4.5.1.5 3-chloro-L-alanine + methylmercaptan 65% of activity compared to thioglycolate Escherichia coli S-methyl-L-cysteine + Cl-
-
 ?
4.5.1.5 3-chloro-L-alanine + methylmercaptan 65% of activity compared to thioglycolate Escherichia coli W 3110 S-methyl-L-cysteine + Cl-
-
 ?
4.5.1.5 3-chloro-L-alanine + n-propylmercaptan 4% of activity compared to thioglycolate Escherichia coli S-propyl-L-cysteine + Cl-
-
 ?
4.5.1.5 3-chloro-L-alanine + phenylmercaptan 207% of activity compared to thioglycolate Escherichia coli S-phenyl-L-cysteine + Cl-
-
 ?
4.5.1.5 3-chloro-L-alanine + thioglycolate beta-replacement reaction Escherichia coli S-carboxymethyl-L-cysteine + Cl-
-
 ?
4.5.1.5 3-chloro-L-alanine + thioglycolate beta-replacement reaction Escherichia coli W 3110 S-carboxymethyl-L-cysteine + Cl-
-
 ?
4.5.1.5 additional information not: L-cysteine, L-serine, L-threonine, L-cystine, 3-chloro-D-alanine, DL-homocysteine, L-methionine, L-homoserine, sodium hydrosulfide, isopropylmercaptan, 2-mercaptopropionic acid, 3-mercaptopropionic acid, enzyme catalyzes the alpha,beta-elimination reaction of 3-chloro-L-alanine, L-serine and L-cystathionine at a very low rate Escherichia coli ?
-
 ?
4.5.1.5 additional information constitutive enzyme Escherichia coli ?
-
 ?
4.5.1.5 additional information not: L-cysteine, L-serine, L-threonine, L-cystine, 3-chloro-D-alanine, DL-homocysteine, L-methionine, L-homoserine, sodium hydrosulfide, isopropylmercaptan, 2-mercaptopropionic acid, 3-mercaptopropionic acid, enzyme catalyzes the alpha,beta-elimination reaction of 3-chloro-L-alanine, L-serine and L-cystathionine at a very low rate Escherichia coli W 3110 ?
-
 ?
4.5.1.5 additional information constitutive enzyme Escherichia coli W 3110 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.5.1.5 homodimer 2 * 37000, SDS-PAGE Escherichia coli
4.5.1.5 homodimer 2 * 42000, HPLC gel filtration in presence of SDS Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.5.1.5 37
-
 assay at Escherichia coli
4.5.1.5 50
-
 S-carboxymethyl-L-cysteine synthesis Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.5.1.5 60
-
 10 min, stable up to, S-carboxymethyl-L-cysteine synthesis Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5.1.5 7.8
-
 assay at Escherichia coli
4.5.1.5 9 10.5 broad, S-carboxymethyl-L-cysteine synthesis Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.5.1.5 6.5 7.5 stable pH-range, S-carboxymethyl-L-cysteine synthesis Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
4.5.1.5 pyridoxal 5'-phosphate as cofactor, a pyridoxal phosphate protein Escherichia coli