EC Number | Crystallization (Comment) | Organism |
---|---|---|
4.5.1.5 | fine yellow needles | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.5.1.5 | additional information | not inhibited by the substrate 3-chloro-L-alanine | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.5.1.5 | 15.4 | - |
thioglycolate | pH 7.8, 37°C | Escherichia coli | |
4.5.1.5 | 40 | - |
3-chloro-L-alanine | pH 7.8, 37°C | Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.5.1.5 | 37000 | - |
2 * 37000, SDS-PAGE | Escherichia coli |
4.5.1.5 | 42000 | - |
2 * 42000, HPLC gel filtration in presence of SDS | Escherichia coli |
4.5.1.5 | 84000 | - |
HPLC gel filtration | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.5.1.5 | additional information | Escherichia coli | constitutive enzyme | ? | - |
? | |
4.5.1.5 | additional information | Escherichia coli W 3110 | constitutive enzyme | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.5.1.5 | Escherichia coli | - |
- |
- |
4.5.1.5 | Escherichia coli W 3110 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.5.1.5 | 190fold | Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.5.1.5 | additional information | - |
- |
Escherichia coli |
EC Number | Storage Stability | Organism |
---|---|---|
4.5.1.5 | long-term storage of enzyme as ammonium sulfate precipitate decreases activity, reactivation by dialysis against 0.1 M potassium phosphate buffer, pH 7.8, containing 10 mM 2-mercaptoethanol, 0.02 mM pyridoxal phosphate, 4 mM EDTA | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.5.1.5 | 3-chloro-L-alanine + 1-thioglycerol | 93% of activity compared to thioglycolate | Escherichia coli | S-2,3-dihydroxypropyl-L-cysteine + Cl- | - |
? | |
4.5.1.5 | 3-chloro-L-alanine + 2-mercaptoethanol | 242% of activity compared to thioglycolate | Escherichia coli | S-2-hydroxyethyl-L-cysteine + Cl- | - |
? | |
4.5.1.5 | 3-chloro-L-alanine + benzylmercaptan | 35% of activity compared to thioglycolate | Escherichia coli | S-benzyl-L-cysteine + Cl- | - |
? | |
4.5.1.5 | 3-chloro-L-alanine + ethylmercaptan | 55% of activity compared to thioglycolate | Escherichia coli | S-ethyl-L-cysteine + Cl- | - |
? | |
4.5.1.5 | 3-chloro-L-alanine + ethylmercaptan | 55% of activity compared to thioglycolate | Escherichia coli W 3110 | S-ethyl-L-cysteine + Cl- | - |
? | |
4.5.1.5 | 3-chloro-L-alanine + methylmercaptan | 65% of activity compared to thioglycolate | Escherichia coli | S-methyl-L-cysteine + Cl- | - |
? | |
4.5.1.5 | 3-chloro-L-alanine + methylmercaptan | 65% of activity compared to thioglycolate | Escherichia coli W 3110 | S-methyl-L-cysteine + Cl- | - |
? | |
4.5.1.5 | 3-chloro-L-alanine + n-propylmercaptan | 4% of activity compared to thioglycolate | Escherichia coli | S-propyl-L-cysteine + Cl- | - |
? | |
4.5.1.5 | 3-chloro-L-alanine + phenylmercaptan | 207% of activity compared to thioglycolate | Escherichia coli | S-phenyl-L-cysteine + Cl- | - |
? | |
4.5.1.5 | 3-chloro-L-alanine + thioglycolate | beta-replacement reaction | Escherichia coli | S-carboxymethyl-L-cysteine + Cl- | - |
? | |
4.5.1.5 | 3-chloro-L-alanine + thioglycolate | beta-replacement reaction | Escherichia coli W 3110 | S-carboxymethyl-L-cysteine + Cl- | - |
? | |
4.5.1.5 | additional information | not: L-cysteine, L-serine, L-threonine, L-cystine, 3-chloro-D-alanine, DL-homocysteine, L-methionine, L-homoserine, sodium hydrosulfide, isopropylmercaptan, 2-mercaptopropionic acid, 3-mercaptopropionic acid, enzyme catalyzes the alpha,beta-elimination reaction of 3-chloro-L-alanine, L-serine and L-cystathionine at a very low rate | Escherichia coli | ? | - |
? | |
4.5.1.5 | additional information | constitutive enzyme | Escherichia coli | ? | - |
? | |
4.5.1.5 | additional information | not: L-cysteine, L-serine, L-threonine, L-cystine, 3-chloro-D-alanine, DL-homocysteine, L-methionine, L-homoserine, sodium hydrosulfide, isopropylmercaptan, 2-mercaptopropionic acid, 3-mercaptopropionic acid, enzyme catalyzes the alpha,beta-elimination reaction of 3-chloro-L-alanine, L-serine and L-cystathionine at a very low rate | Escherichia coli W 3110 | ? | - |
? | |
4.5.1.5 | additional information | constitutive enzyme | Escherichia coli W 3110 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.5.1.5 | homodimer | 2 * 37000, SDS-PAGE | Escherichia coli |
4.5.1.5 | homodimer | 2 * 42000, HPLC gel filtration in presence of SDS | Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.5.1.5 | 37 | - |
assay at | Escherichia coli |
4.5.1.5 | 50 | - |
S-carboxymethyl-L-cysteine synthesis | Escherichia coli |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.5.1.5 | 60 | - |
10 min, stable up to, S-carboxymethyl-L-cysteine synthesis | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.5.1.5 | 7.8 | - |
assay at | Escherichia coli |
4.5.1.5 | 9 | 10.5 | broad, S-carboxymethyl-L-cysteine synthesis | Escherichia coli |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
4.5.1.5 | 6.5 | 7.5 | stable pH-range, S-carboxymethyl-L-cysteine synthesis | Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.5.1.5 | pyridoxal 5'-phosphate | as cofactor, a pyridoxal phosphate protein | Escherichia coli |