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Literature summary extracted from

  • Mihara, H.; Kurihara, T.; Watanabe, T.; Yoshimura, T.; Esaki, N.
    cDNA cloning, purification, and characterization of mouse liver selenocysteine lyase. Candidate for selenium delivery protein in selenoprotein synthesis (2000), J. Biol. Chem., 275, 6195-6200.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.16 expressed in Escherichia coli BL21(DE3) Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.16 8.6
-
 L-cysteine sulfinate pH 9.0, 37°C Mus musculus
4.4.1.16 9.9
-
 L-selenocysteine pH 9.0, 37°C Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.4.1.16 cytoplasm
-
 Mus musculus 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.16 47000
-
 SDS-PAGE Mus musculus
4.4.1.16 47200
-
 calculated from amino acid sequence Mus musculus
4.4.1.16 105000
-
 gel filtration Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.16 L-selenocysteine + reduced acceptor Sus scrofa probably functions as selenide delivery system selenide + L-alanine + acceptor
-
 ?
4.4.1.16 L-selenocysteine + reduced acceptor Mus musculus probably functions as selenide delivery system selenide + L-alanine + acceptor
-
 ?
4.4.1.16 L-selenocysteine + reduced acceptor Sus scrofa most likely metabolizes small amounts of selenocysteine in tissue selenide + L-alanine + acceptor
-
 ?
4.4.1.16 L-selenocysteine + reduced acceptor Mus musculus most likely metabolizes small amounts of selenocysteine in tissue selenide + L-alanine + acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.16 Mus musculus Q9JLI6
-
-
4.4.1.16 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.16
-
 Sus scrofa
4.4.1.16 overexpressed protein Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.4.1.16 brain
-
 Mus musculus
-
4.4.1.16 heart
-
 Sus scrofa
-
4.4.1.16 heart
-
 Mus musculus
-
4.4.1.16 kidney
-
 Sus scrofa
-
4.4.1.16 kidney
-
 Mus musculus
-
4.4.1.16 liver
-
 Sus scrofa
-
4.4.1.16 liver
-
 Mus musculus
-
4.4.1.16 lung
-
 Mus musculus
-
4.4.1.16 spleen
-
 Mus musculus
-
4.4.1.16 stomach
-
 Mus musculus
-
4.4.1.16 testis
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.16 29
-
 purified enzyme, pH 7.4 Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.16 L-cysteine sulfinic acid + reduced acceptor less than 10% of activity Mus musculus SO2 + L-alanine + acceptor
-
 ?
4.4.1.16 L-selenocysteine + reduced acceptor
-
 Sus scrofa selenide + L-alanine + acceptor
-
 ?
4.4.1.16 L-selenocysteine + reduced acceptor
-
 Mus musculus selenide + L-alanine + acceptor
-
 ?
4.4.1.16 L-selenocysteine + reduced acceptor probably functions as selenide delivery system Sus scrofa selenide + L-alanine + acceptor
-
 ?
4.4.1.16 L-selenocysteine + reduced acceptor probably functions as selenide delivery system Mus musculus selenide + L-alanine + acceptor
-
 ?
4.4.1.16 L-selenocysteine + reduced acceptor most likely metabolizes small amounts of selenocysteine in tissue Sus scrofa selenide + L-alanine + acceptor
-
 ?
4.4.1.16 L-selenocysteine + reduced acceptor most likely metabolizes small amounts of selenocysteine in tissue Mus musculus selenide + L-alanine + acceptor
-
 ?
4.4.1.16 additional information
-
 Sus scrofa ?
-
 ?
4.4.1.16 additional information traces of activity with L-cysteine Mus musculus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.4.1.16 dimer
-
 Sus scrofa
4.4.1.16 dimer 2 * 47000, SDS-PAGE Mus musculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.4.1.16 0.35
-
 L-cysteine sulfinate pH 9.0, 37°C Mus musculus
4.4.1.16 46
-
 L-selenocysteine pH 9.0, 37°C Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4.1.16 9
-
-
 Sus scrofa
4.4.1.16 9
-
-
 Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
4.4.1.16 pyridoxal 5'-phosphate required Sus scrofa
4.4.1.16 pyridoxal 5'-phosphate required Mus musculus