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Literature summary extracted from

  • Schmidt, A.; Erdle, I.
    A cysteine desulfhydrase specific for D-cysteine from the green alga Chlorella fusca (1983), Z. Naturforsch. C, 38 c, 428-435.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.4.1.15 Fe2+
-
 [Chlorella] fusca
4.4.1.15 Ni2+
-
 [Chlorella] fusca
4.4.1.15 Zn2+
-
 [Chlorella] fusca

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.15 0.16
-
 D-cysteine 37°C, pH 9.0 [Chlorella] fusca

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.15 D-cysteine + H2O [Chlorella] fusca
-
 sulfide + NH3 + pyruvate additional product suggested ?
4.4.1.15 D-cysteine + H2O [Chlorella] fusca
-
 sulfide + NH3 + pyruvate more sulfide than pyruvate and ammonium formed ?

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.15 [Chlorella] fusca
-
 green alga, strain 211-8b, formerly Chlorella pyrenoidosa strain 211-8b
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.15
-
 [Chlorella] fusca

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.15 0.21
-
 purified enzyme, 37°C, pH 9.0 [Chlorella] fusca

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.15 D-cysteine + H2O
-
 [Chlorella] fusca sulfide + NH3 + pyruvate additional product suggested ?
4.4.1.15 D-cysteine + H2O
-
 [Chlorella] fusca sulfide + NH3 + pyruvate more sulfide than pyruvate and ammonium formed ?
4.4.1.15 D-cysteine + H2O very specific, only traces of activity with mercaptoacetic acid, mercaptoethanol, D-cystine, L-cystine, cysteamine, L-cysteine methylester [Chlorella] fusca sulfide + NH3 + pyruvate additional product suggested ?
4.4.1.15 D-cysteine + H2O very specific, only traces of activity with mercaptoacetic acid, mercaptoethanol, D-cystine, L-cystine, cysteamine, L-cysteine methylester [Chlorella] fusca sulfide + NH3 + pyruvate more sulfide than pyruvate and ammonium formed ?
4.4.1.15 additional information L-cysteine is not utilized [Chlorella] fusca ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4.1.15 8.5 9
-
 [Chlorella] fusca

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.4.1.15 7 11
-
 [Chlorella] fusca