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Literature summary extracted from

  • Kolhekar, A.S.; Bell, J.; Shiozaki, E.N.; Jin, L.; Keutmann, H.T.; Hand, T.A.; Mains, R.E.; Eipper, B.A.
    Essential features of the catalytic core of peptidyl-a-hydroxyglycine alpha-amidating Lyase (2002), Biochemistry, 41, 12384-12394.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.2.5 expression in Chinese hamster ovary cells Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
4.3.2.5 D590N no secretion Rattus norvegicus
4.3.2.5 D599N no secretion Rattus norvegicus
4.3.2.5 D652N no secretion Rattus norvegicus
4.3.2.5 D705N much less secretion and less active than wild-type Rattus norvegicus
4.3.2.5 E707Q much less secretion than wild-type Rattus norvegicus
4.3.2.5 H532A no secretion Rattus norvegicus
4.3.2.5 H585A less active than wild-type Rattus norvegicus
4.3.2.5 H603A no secretion Rattus norvegicus
4.3.2.5 H690A less active than wild-type Rattus norvegicus
4.3.2.5 H697A no changes in secretion an activity Rattus norvegicus
4.3.2.5 H786A less active than wild-type Rattus norvegicus
4.3.2.5 H817A no changes in secretion an activity Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.2.5 2-mercaptoethanol
-
 Rattus norvegicus
4.3.2.5 EDTA addition of 0.5-1 M reduces activity to 50%, Ca2+, Mn2+, Co2+ or Ni2+ restore activity Rattus norvegicus
4.3.2.5 EGTA addition of 0.5-1 M reduces activity to 50%, Ca2+, Mn2+, Co2+ or Ni2+ restore activity Rattus norvegicus
4.3.2.5 HCl 0.5 M guanidinium HCl reduces activity to 28% Rattus norvegicus
4.3.2.5 o-phenanthroline
-
 Rattus norvegicus
4.3.2.5 Urea addition of 1 M urea reduces activity to 58% Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.2.5 0.0102
-
 Nalpha-Acetyl-Tyr-Val-alpha-hydroxyglycine 37°C, pH 5.5, E707Q Rattus norvegicus
4.3.2.5 0.0139
-
 Nalpha-Acetyl-Tyr-Val-alpha-hydroxyglycine 37°C, pH 5.5, recombinant wild-type Rattus norvegicus
4.3.2.5 0.0156
-
 Nalpha-Acetyl-Tyr-Val-alpha-hydroxyglycine 37°C, pH 5.5, H690A Rattus norvegicus
4.3.2.5 0.0214
-
 Nalpha-Acetyl-Tyr-Val-alpha-hydroxyglycine 37°C, pH 5.5, D705N Rattus norvegicus
4.3.2.5 0.0231
-
 Nalpha-Acetyl-Tyr-Val-alpha-hydroxyglycine 37°C, pH 5.5, H585A Rattus norvegicus
4.3.2.5 0.0985
-
 Nalpha-Acetyl-Tyr-Val-alpha-hydroxyglycine 37°C, pH 5.5, H786A Rattus norvegicus
4.3.2.5 0.33
-
 Nalpha-Acetyl-Tyr-Val-alpha-hydroxyglycine 37°C, pH 5.5 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.3.2.5 extracellular
-
 Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.3.2.5 Zn2+ 0.7 mol per mol enzyme Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.2.5 38000
-
 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
4.3.2.5 Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.2.5 Nalpha-acetyl-Tyr-Val-alpha-hydroxyglycine
-
 Rattus norvegicus Nalpha-acetyl-Tyr-Val-NH2 + glyoxylate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.3.2.5 380
-
 Nalpha-Acetyl-Tyr-Val-alpha-hydroxyglycine 37°C, pH 5.5 Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.2.5 5 6.5
-
 Rattus norvegicus