EC Number | General Stability | Organism |
---|---|---|
4.2.99.18 | Escherichia coli enzyme is more sensitive to SDS, sodium deoxycholate or urea than the Bacillus stearothermophilus enzyme | Geobacillus stearothermophilus |
4.2.99.18 | stable up to 2% Triton X-100 | Geobacillus stearothermophilus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.2.99.18 | Ca2+ | 39% inhibition at 10 mM | Geobacillus stearothermophilus | |
4.2.99.18 | Co2+ | 86% inhibition at 10 mM | Geobacillus stearothermophilus | |
4.2.99.18 | Cu2+ | 90% inhibition at 10 mM | Geobacillus stearothermophilus | |
4.2.99.18 | EDTA | no inhibition | Geobacillus stearothermophilus | |
4.2.99.18 | Mg2+ | 57% inhibition at 10 mM | Geobacillus stearothermophilus | |
4.2.99.18 | Mn2+ | 37% inhibition at 10 mM | Geobacillus stearothermophilus | |
4.2.99.18 | Zn2+ | 86% inhibition at 10 mM | Geobacillus stearothermophilus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.2.99.18 | KCl | stimulates | Geobacillus stearothermophilus | |
4.2.99.18 | KCl | optimal concentration: 125 mM | Geobacillus stearothermophilus | |
4.2.99.18 | NaCl | stimulates | Geobacillus stearothermophilus | |
4.2.99.18 | NaCl | optimal concentration: 50 mM | Geobacillus stearothermophilus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.2.99.18 | 27000 | - |
gel filtration | Geobacillus stearothermophilus |
4.2.99.18 | 28000 | - |
1 * 28000, SDS-PAGE | Geobacillus stearothermophilus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.99.18 | DNA | Geobacillus stearothermophilus | - |
fragments of DNA | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.99.18 | Geobacillus stearothermophilus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.99.18 | - |
Geobacillus stearothermophilus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.2.99.18 | additional information | - |
- |
Geobacillus stearothermophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.99.18 | DNA | - |
Geobacillus stearothermophilus | fragments of DNA | - |
? | |
4.2.99.18 | DNA | specific for apurinic sites | Geobacillus stearothermophilus | fragments of DNA | - |
? | |
4.2.99.18 | additional information | no substrate: alkylated sites | Geobacillus stearothermophilus | ? | - |
? | |
4.2.99.18 | additional information | no substrate: native DNA | Geobacillus stearothermophilus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.2.99.18 | monomer | 1 * 28000, SDS-PAGE | Geobacillus stearothermophilus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.99.18 | 60 | - |
- |
Geobacillus stearothermophilus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.99.18 | 60 | - |
with 0.4% bovine serum albumin stable for 120 min | Geobacillus stearothermophilus |