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Literature summary extracted from

  • Dueber, M.T.; Adolf, W.; West, C.A.
    Biosynthesis of diterpene phytoalexin casbene: partial purification and characterization of casbene synthetase from Rhizinus communis (1978), Plant Physiol., 62, 598-603.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.3.8 Mn2+ above 0.2 mM Ricinus communis
4.2.3.8 additional information 10 mM iodoacetamide is not inhibitory, SKF525A, N,N-dimethylaminoethyl-2,2-diphenylpentanoate and the growth retardant Amo-1618, 2'-isopropyl-4'-(trimethylammonium chloride)-5'-methylphenyl piperidine-1-carboxylate are ineffective inhibitors Ricinus communis
4.2.3.8 N-ethylmaleimide activity is inhibited by 50% Ricinus communis
4.2.3.8 Tributyl-2,4-dichlorobenzylphosphonium chloride phosphonD, growth retardant, inhibits the activity by 55% Ricinus communis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.8 0.0019
-
 geranylgeranyl diphosphate pH 9.0, 30°C Ricinus communis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.3.8 cytoplasm
-
 Ricinus communis 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.8 Mg2+ highly dependent on, maximal stimulation of the activity above 5 mM, Mn2+ is much less effective Ricinus communis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.8 53000
-
 native enzyme, gel filtration Ricinus communis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.8 geranylgeranyl diphosphate Ricinus communis regulation of phytoalexin biosynthesis, enzyme produces the antifungal diterpene casbene, a stress metabolite casbene + diphosphate diterpene phytoalexin, product of a single enzyme-catalyzed transformation ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.8 Ricinus communis
-
 Hale variety, N5 variety
-
4.2.3.8 Ricinus communis
-
 castor bean, unknown variety
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.8 partial Ricinus communis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.8 seedling infected with the fungus Rhizpus stolonifer, no detectable levels of casbene synthetase mRNA in healthy seedlings Ricinus communis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.3.8 0.0437
-
-
 Ricinus communis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.8 geranylgeranyl diphosphate
-
 Ricinus communis casbene + diphosphate
-
 ?
4.2.3.8 geranylgeranyl diphosphate regulation of phytoalexin biosynthesis, enzyme produces the antifungal diterpene casbene, a stress metabolite Ricinus communis casbene + diphosphate diterpene phytoalexin, product of a single enzyme-catalyzed transformation ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.8 7.5 9 half-maximal activity at pH 6.0 and 9.8 Ricinus communis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.3.8 6 9.8 half-maximal activity at pH 6.0 and 9.8 Ricinus communis