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Literature summary extracted from

  • Hashimoto, W.; Miki, H.; Tsuchiya, N.; Nankai, H.; Murata, K.
    Xanthan lyase of Bacillus sp. strain GL1 liberates pyruvylated mannose from xanthan side chains (1998), Appl. Environ. Microbiol., 64, 3765-3768.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.2.12 2-mercaptoethanol 1 mM, slight stimulation Bacillus sp. (in: Bacteria)

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.12 EDTA 1 mM, 30% loss of activity Bacillus sp. (in: Bacteria)
4.2.2.12 HgCl2 1 mM, 95% loss of activity Bacillus sp. (in: Bacteria)
4.2.2.12 iodoacetamide 1 mM, 70% loss of activity Bacillus sp. (in: Bacteria)
4.2.2.12 NaCl 150 mM, 70% loss of activity Bacillus sp. (in: Bacteria)

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.2.12 soluble excreted to medium Bacillus sp. (in: Bacteria)
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.12 Co2+ 1 mM, slight stimulation Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.12 75000
-
gel filtration Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.12 Bacillus sp. (in: Bacteria)
-
enzyme is induced by xanthan
-
4.2.2.12 Bacillus sp. (in: Bacteria) GL1
-
enzyme is induced by xanthan
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.12
-
Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.12 6.28
-
pH 7.0 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.12 pyruvylated xanthan best substrate Bacillus sp. (in: Bacteria) pyruvylate mannose + oligosaccharides
-
?
4.2.2.12 pyruvylated xanthan best substrate Bacillus sp. (in: Bacteria) GL1 pyruvylate mannose + oligosaccharides
-
?
4.2.2.12 xanthan
-
Bacillus sp. (in: Bacteria) oligosaccharides
-
?
4.2.2.12 xanthan
-
Bacillus sp. (in: Bacteria) GL1 oligosaccharides
-
?

Subunits

EC Number Subunits Comment Organism
4.2.2.12 monomer 1 * 75000, SDS-PAGE   Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.12 50
-
-
Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.12 45
-
pH 7.0, 10 min, loss of 60% of activity Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.12 5.5
-
-
Bacillus sp. (in: Bacteria)

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.12 6.5 9 at 4°C Bacillus sp. (in: Bacteria)