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Literature summary extracted from

  • Iwamoto, Y.; Araki, R.; Iriyama, K.; Oda, T.; Fukuda, H.; Hayashida, S.; Muramatsu, T.
    Purification and characterization of bifunctional alginate lyase from Alteromonas sp. strain no. 272 and its action on saturated oligomeric substrates (2001), Biosci. Biotechnol. Biochem., 65, 133-142.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.2.2.11 more labile to heat treatment in Tris-HCl buffer, pH 7.0 than in phosphate buffer with similar pH Alteromonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.3 Ba2+
-
 Alteromonas sp.
4.2.2.3 Cd2+
-
 Alteromonas sp.
4.2.2.3 Ni2+
-
 Alteromonas sp.
4.2.2.3 Zn2+
-
 Alteromonas sp.
4.2.2.11 Ba(OH)2 slightly inhibitory, percentage of inhibition is dependent on substrate Alteromonas sp.
4.2.2.11 NiSO4 slightly inhibitory, percentage of inhibition is dependent on substrate Alteromonas sp.
4.2.2.11 ZnCl2 slightly inhibitory, percentage of inhibition is dependent on substrate Alteromonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.3 0.025
-
 nona-alpha1,4-L-guluronate pH 8.5, 30°C Alteromonas sp.
4.2.2.3 0.028
-
 octa-alpha1,4-L-guluronate pH 8.5, 30°C Alteromonas sp.
4.2.2.3 0.031
-
 hepta-alpha1,4-L-guluronate pH 8.5, 30°C Alteromonas sp.
4.2.2.3 0.035
-
 hexa-alpha1,4-L-guluronate pH 8.5, 30°C Alteromonas sp.
4.2.2.3 0.039
-
 penta-alpha1,4-L-guluronate pH 8.5, 30°C Alteromonas sp.
4.2.2.3 0.172
-
 nona-beta1,4-D-mannuronate pH 8.5, 30°C Alteromonas sp.
4.2.2.3 0.186
-
 octa-beta1,4-D-mannuronate pH 8.5, 30°C Alteromonas sp.
4.2.2.3 0.192
-
 hepta-beta1,4-D-mannuronate pH 8.5, 30°C Alteromonas sp.
4.2.2.3 0.226
-
 hexa-beta1,4-D-mannuronate pH 8.5, 30°C Alteromonas sp.
4.2.2.3 0.232
-
 penta-beta1,4-D-mannuronate pH 8.5, 30°C Alteromonas sp.
4.2.2.3 3.86
-
 tetra-alpha1,4-L-guluronate pH 8.5, 30°C Alteromonas sp.
4.2.2.3 4.25
-
 tetra-beta1,4-D-mannuronate pH 8.5, 30°C Alteromonas sp.
4.2.2.11 0.192
-
 saturated hepta((1-4)-alpha-L-guluronan) pH 8.5, 30 °C, 100 mM glycine in 0.1 N NaCl and 0.1 N NaOH Alteromonas sp.
4.2.2.11 0.226
-
 saturated hexa((1-4)-alpha-L-guluronan) pH 8.5, 30 °C, 100 mM glycine in 0.1 N NaCl and 0.1 N NaOH Alteromonas sp.
4.2.2.11 0.232
-
 saturated penta((1-4)-alpha-L-guluronan) pH 8.5, 30 °C, 100 mM glycine in 0.1 N NaCl and 0.1 N NaOH Alteromonas sp.
4.2.2.11 4.25
-
 saturated tetra((1-4)-alpha-L-guluronan) pH 8.5, 30 °C, 100 mM glycine in 0.1 N NaCl and 0.1 N NaOH Alteromonas sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.2.3 extracellular secretion to the culture medium Alteromonas sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.3 Co2+
-
 Alteromonas sp.
4.2.2.3 K+ slightly activating Alteromonas sp.
4.2.2.3 Mg2+ slightly activating Alteromonas sp.
4.2.2.3 Mn2+ slightly activating Alteromonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.3 23000
-
 gel filtration Alteromonas sp.
4.2.2.3 33900
-
 1 * 33900, SDS-PAGE Alteromonas sp.
4.2.2.11 23000
-
 gel filtration Alteromonas sp.
4.2.2.11 33900
-
 x * 33900, SDS-PAGE Alteromonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.2.11 poly-alpha-L-guluronic acid Alteromonas sp.
-
 unsaturated 1,4-di-, 1,4-tri- and 1,4-tetrasaccharides of L-guluronate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.3 Alteromonas sp.
-
 bifunctional enzyme, mannuronate- and guluronate-specific alginate lyase
-
4.2.2.3 Alteromonas sp. 272
-
 bifunctional enzyme, mannuronate- and guluronate-specific alginate lyase
-
4.2.2.11 Alteromonas sp.
-
 strain No. 272
-
4.2.2.11 Alteromonas sp. 272
-
 strain No. 272
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.3 from the culture medium, 135fold, to homogeneity Alteromonas sp.
4.2.2.11 anion exchange chromatography, and Sephadex G-100 chromatography Alteromonas sp.

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.2.3 R2-beta-D-mannuronic acid-R1 = R2-OH + 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-R1 enzyme seems to have 6 substrate binding sites, also performs reaction of EC 4.2.2.11 Alteromonas sp.
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.2.3 culture medium
-
 Alteromonas sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.3 hepta-alpha1,4-L-guluronate
-
 Alteromonas sp. 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate
-
 ?
4.2.2.3 hepta-alpha1,4-L-guluronic acid
-
 Alteromonas sp. 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate
-
 ?
4.2.2.3 hepta-alpha1,4-L-guluronic acid
-
 Alteromonas sp. 272 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate
-
 ?
4.2.2.3 hepta-beta1,4-D-mannuronic acid
-
 Alteromonas sp. D-mannuronic acid
-
 ?
4.2.2.3 hexa-alpha1,4-L-guluronate
-
 Alteromonas sp. 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate
-
 ?
4.2.2.3 hexa-alpha1,4-L-guluronic acid
-
 Alteromonas sp. 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate
-
 ?
4.2.2.3 hexa-alpha1,4-L-guluronic acid
-
 Alteromonas sp. 272 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate
-
 ?
4.2.2.3 hexa-beta1,4-D-mannuronic acid
-
 Alteromonas sp. D-mannuronic acid
-
 ?
4.2.2.3 additional information no activity with triguluronate and trimannuronate, but with tetramers or substrates with longer chain length Alteromonas sp. ?
-
 ?
4.2.2.3 additional information no activity with triguluronate and trimannuronate, but with tetramers or substrates with longer chain length Alteromonas sp. 272 ?
-
 ?
4.2.2.3 nona-alpha1,4-L-guluronate
-
 Alteromonas sp. 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate
-
 ?
4.2.2.3 nona-alpha1,4-L-guluronic acid
-
 Alteromonas sp. 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate
-
 ?
4.2.2.3 nona-beta1,4-D-mannuronic acid
-
 Alteromonas sp. D-mannuronic acid
-
 ?
4.2.2.3 octa-alpha1,4-L-guluronate
-
 Alteromonas sp. 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate
-
 ?
4.2.2.3 octa-alpha1,4-L-guluronic acid
-
 Alteromonas sp. 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate
-
 ?
4.2.2.3 octa-beta1,4-D-mannuronate
-
 Alteromonas sp. D-mannuronic acid
-
 ?
4.2.2.3 octa-beta1,4-D-mannuronic acid
-
 Alteromonas sp. D-mannuronic acid
-
 ?
4.2.2.3 octa-beta1,4-D-mannuronic acid
-
 Alteromonas sp. D-mannuronic acid + ?
-
 ?
4.2.2.3 penta-alpha1,4-L-guluronate
-
 Alteromonas sp. 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate
-
 ?
4.2.2.3 penta-alpha1,4-L-guluronic acid
-
 Alteromonas sp. 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate
-
 ?
4.2.2.3 penta-alpha1,4-L-guluronic acid
-
 Alteromonas sp. 272 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate
-
 ?
4.2.2.3 penta-beta1,4-D-mannuronic acid
-
 Alteromonas sp. 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-beta-1,4-oligo-D-mannuronate + D-mannuronate
-
 ?
4.2.2.3 poly-alpha1,4-L-guluronic acid competitive to poly-beta1,4-D-mannuronic acid Alteromonas sp. ?
-
 ?
4.2.2.3 poly-beta1,4-D-mannuronic acid competitive to poly-alpha1,4L-guluronic acid Alteromonas sp. ?
-
 ?
4.2.2.3 sodium alginate endo-type manner Alteromonas sp. ?
-
 ?
4.2.2.3 tetra-alpha1,4-L-guluronate
-
 Alteromonas sp. 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate
-
 ?
4.2.2.3 tetra-alpha1,4-L-guluronic acid
-
 Alteromonas sp. 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate
-
 ?
4.2.2.3 tetra-alpha1,4-L-guluronic acid
-
 Alteromonas sp. 272 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate
-
 ?
4.2.2.3 tetra-beta1,4-D-mannuronic acid
-
 Alteromonas sp. 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-beta-1,4-D-mannuronoyl-beta-1,4-D-mannuranote + D-mannuronate
-
 ?
4.2.2.11 alpha-L-guluronosyl linkage in alginate endolyase Alteromonas sp. ?
-
 ?
4.2.2.11 additional information no action on trimeric guluronan and mannuronan, but on tetramers or more, the enzyme is most likely beta-structure, the subsite number is most likely six for both guluronate and mannuronate units, the catalytic site of this enzyme is located at the midpoint of the subsite Alteromonas sp. ?
-
 ?
4.2.2.11 poly-alpha-L-guluronic acid
-
 Alteromonas sp. ?
-
 ?
4.2.2.11 poly-alpha-L-guluronic acid
-
 Alteromonas sp. unsaturated 1,4-di-, 1,4-tri- and 1,4-tetrasaccharides of L-guluronate
-
 ?
4.2.2.11 poly-beta1,4-D-mannuronan competitive to poly-alpha1,4-L-guluronan Alteromonas sp. ?
-
 ?
4.2.2.11 saturated hepta((1-4)-alpha-L-guluronan)
-
 Alteromonas sp. ?
-
 ?
4.2.2.11 saturated hexa((1-4)-alpha-L-guluronan)
-
 Alteromonas sp. unsaturated tetramer and a saturated dimer
-
 ?
4.2.2.11 saturated penta((1-4)-alpha-L-guluronan)
-
 Alteromonas sp. ?
-
 ?
4.2.2.11 saturated tetra((1-4)-alpha-L-guluronan) only reactive in a high concentration of enzyme, and for a prolonged reaction time Alteromonas sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.3 monomer 1 * 33900, SDS-PAGE Alteromonas sp.
4.2.2.11 ? x * 33900, SDS-PAGE Alteromonas sp.

Synonyms

EC Number Synonyms Comment Organism
4.2.2.3 alginate lyase
-
 Alteromonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.3 30
-
 assay at Alteromonas sp.
4.2.2.11 30
-
 assay at Alteromonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.3 additional information
-
-
 Alteromonas sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.2.3 2.5
-
 tetra-alpha1,4-L-guluronic acid pH 8.5, 30°C Alteromonas sp.
4.2.2.3 3.2
-
 tetra-beta1,4-D-mannuronate pH 8.5, 30°C Alteromonas sp.
4.2.2.3 6.2
-
 penta-alpha1,4-L-guluronic acid pH 8.5, 30°C Alteromonas sp.
4.2.2.3 7.3
-
 octa-alpha1,4-L-guluronic acid pH 8.5, 30°C Alteromonas sp.
4.2.2.3 7.4
-
 penta-beta1,4-D-mannuronate pH 8.5, 30°C Alteromonas sp.
4.2.2.3 7.6
-
 hepta-alpha1,4-L-guluronic acid pH 8.5, 30°C Alteromonas sp.
4.2.2.3 7.8
-
 nona-alpha1,4-L-guluronic acid pH 8.5, 30°C Alteromonas sp.
4.2.2.3 7.9
-
 hexa-alpha1,4-L-guluronic acid pH 8.5, 30°C Alteromonas sp.
4.2.2.3 10.8
-
 nona-beta1,4-D-mannuronate pH 8.5, 30°C Alteromonas sp.
4.2.2.3 11.1
-
 octa-beta1,4-D-mannuronic acid pH 8.5, 30°C Alteromonas sp.
4.2.2.3 11.9
-
 hexa-beta1,4-D-mannuronate pH 8.5, 30°C Alteromonas sp.
4.2.2.3 13.2
-
 hepta-beta1,4-D-mannuronate pH 8.5, 30°C Alteromonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.3 7.5 8
-
 Alteromonas sp.
4.2.2.11 8.5
-
 assay at Alteromonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.11 7.5 8 maximal activity, slightly dependent on buffer composition Alteromonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.2.11 5 11 stable Alteromonas sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.2.3 Pseudoalteromonas elyakovii isoelectric focusing
-
 3.8
4.2.2.11 Alteromonas sp.
-
-
 3.8