Literature summary extracted from

Pereira, R.A.; Graham, D.; Rainey, F.A.; Cowan, D.A.
A novel thermostable nitrile hydratase (1998), Extremophiles, 2, 347-357.

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
4.2.1.84	110000	-	-	Bacillus sp. (in: Bacteria)

Organism

EC Number	Organism	UniProt	Comment	Textmining
4.2.1.84	Bacillus sp. (in: Bacteria)	-	strain DSM 2349	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4.2.1.84	acetonitrile + H2O	-	Bacillus sp. (in: Bacteria)	acetamide	-	r
4.2.1.84	additional information	an unusual preference for branched and cyclic aliphatic nitriles is noted	Bacillus sp. (in: Bacteria)	?	-	?
4.2.1.84	valeronitrile + H2O	-	Bacillus sp. (in: Bacteria)	valeramide	-	r

Subunits

EC Number	Subunits	Comment	Organism
4.2.1.84	tetramer	a2b2 heterotetramer	Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number	Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
4.2.1.84	60	-	stable in absence of either substrates or analogs	Bacillus sp. (in: Bacteria)

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
4.2.1.84	12.4	-	acetonitrile	-	Bacillus sp. (in: Bacteria)
4.2.1.84	76.3	-	Valeronitrile	-	Bacillus sp. (in: Bacteria)

pH Range

EC Number	pH Minimum	pH Maximum	Comment	Organism
4.2.1.84	5.1	8.7	about 50% of maximum activity	Bacillus sp. (in: Bacteria)

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Release 2023.1 (January 2023)