Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Stevens, J.M.; Rao Saroja, N.; Jaouen, M.; Belghazi, M.; Schmitter, J-M.; Mansuy, D.; Artaud, I.; Sari, M-A.
    Chaperone-assisted expression, purification, and characterization of recombinant nitrile hydratase NI1 from Comamonas testosteroni (2003), Protein Expr. Purif., 29, 70-76.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.84 expression in Escherichia coli Comamonas testosteroni

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.84 0.25
-
 2-nitro-5-thiocyanato-benzoic acid pH 7.2, 28ºC, recombinant enzyme Comamonas testosteroni
4.2.1.84 0.6
-
 2-nitro-5-thiocyanato-benzoic acid pH 7.2, 28ºC, native enzyme Comamonas testosteroni
4.2.1.84 2.5
-
 Cyanovaleric acid pH 7.2, 28ºC, native enzyme Comamonas testosteroni
4.2.1.84 3.2
-
 Cyanovaleric acid pH 7.2, 28ºC, recombinant enzyme Comamonas testosteroni

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.84 Fe2+ ferric citrate has no effect on the enzyme activity Comamonas testosteroni

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.84 22700
-
 2 * 22800, alpha + 2 * 22700, beta, SDS-PAGE Comamonas testosteroni
4.2.1.84 22800
-
 2 * 22800, alpha + 2 * 22700, beta, SDS-PAGE Comamonas testosteroni
4.2.1.84 84000
-
 gel filtration Comamonas testosteroni

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.84 Comamonas testosteroni
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.84 chromatography on Q-Sepharose, phenyl-Sepharose and Uno Q Comamonas testosteroni

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.84 6.8
-
 recombinant enzyme, with 2-nitro-5-thiocyanato-benzoic acid Comamonas testosteroni
4.2.1.84 7.7
-
 native enzyme, with 2-nitro-5-thiocyanato-benzoic acid Comamonas testosteroni
4.2.1.84 95
-
 native enzyme, with cyanovaleric acid Comamonas testosteroni
4.2.1.84 100
-
 recombinant enzyme, with cyanovaleric acid Comamonas testosteroni

Storage Stability

EC Number Storage Stability Organism
4.2.1.84 -40ºC, pH 7.2, 40 mM butyric acid, stable Comamonas testosteroni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.84 2-nitro-5-thiocyanato-benzoic acid + H2O
-
 Comamonas testosteroni ?
-
 ?
4.2.1.84 cyanovaleric acid + H2O
-
 Comamonas testosteroni ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.84 ?
-
 Comamonas testosteroni
4.2.1.84 tetramer 2 * 22800, alpha + 2 * 22700, beta, SDS-PAGE Comamonas testosteroni