EC Number | General Stability | Organism |
---|---|---|
4.2.1.84 | organic acids stabilize, stable for more than 1 month in 0.1 M HEPES/KOH, pH 7.2, with 44 mM n-butyric acid, n-valeric acid, isovaleric acid, isobutyric acid or n-caproic acid | Rhodococcus rhodochrous |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.84 | Ag+ | 0.01-1 mM AgNO3 | Rhodococcus rhodochrous | |
4.2.1.84 | diethyldithiocarbamate | 0.1 mM | Rhodococcus rhodochrous | |
4.2.1.84 | Hg2+ | HgCl2, 0.01-1 mM | Rhodococcus rhodochrous | |
4.2.1.84 | hydroxylamine | 1 mM | Rhodococcus rhodochrous | |
4.2.1.84 | KCN | 0.01 mM | Rhodococcus rhodochrous | |
4.2.1.84 | Na2S2O4 | 1 mM | Rhodococcus rhodochrous | |
4.2.1.84 | phenylhydrazine | 1 mM | Rhodococcus rhodochrous | |
4.2.1.84 | Semicarbazide | 1 mM, weak inhibition | Rhodococcus rhodochrous |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.84 | 1.85 | - |
Isobutyronitrile | pH 7.0, 20ºC, H-NHase | Rhodococcus rhodochrous | |
4.2.1.84 | 1.89 | - |
acrylonitrile | pH 7.0, 20ºC, H-NHase | Rhodococcus rhodochrous | |
4.2.1.84 | 1.92 | - |
Propionitrile | pH 7.0, 20ºC, H-NHase | Rhodococcus rhodochrous | |
4.2.1.84 | 4.88 | - |
Crotononitrile | pH 7.0, 20ºC, H-NHase | Rhodococcus rhodochrous | |
4.2.1.84 | 6.76 | - |
Methacrylonitrile | pH 7.0, 20ºC | Rhodococcus rhodochrous | |
4.2.1.84 | 10 | - |
Cyanopyrazine | pH 7.0, 20ºC | Rhodococcus rhodochrous | |
4.2.1.84 | 12.2 | - |
Chloroacetonitrile | pH 7.0, 20ºC, H-NHase | Rhodococcus rhodochrous | |
4.2.1.84 | 18.5 | - |
4-cyanopyridine | pH 7.0, 20ºC, H-NHase | Rhodococcus rhodochrous | |
4.2.1.84 | 21.7 | - |
2-Cyanopyridine | pH 7.0, 20ºC, H-NHase | Rhodococcus rhodochrous | |
4.2.1.84 | 21.7 | - |
n-butyronitrile | pH 7.0, 20ºC, H-NHase | Rhodococcus rhodochrous | |
4.2.1.84 | 28.6 | - |
Benzonitrile | pH 7.0, 20ºC, H-NHase | Rhodococcus rhodochrous | |
4.2.1.84 | 200 | - |
3-Cyanopyridine | pH 7.0, 20ºC, H-NHase | Rhodococcus rhodochrous |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.84 | Co2+ | contains approximately 11-12 mol cobalt/mol enzyme | Rhodococcus rhodochrous |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.2.1.84 | 26000 | - |
x * 26000, alpha, + x * 29000, beta, SDS-PAGE | Rhodococcus rhodochrous |
4.2.1.84 | 29000 | - |
x * 26000, alpha, + x * 29000, beta, SDS-PAGE | Rhodococcus rhodochrous |
4.2.1.84 | 505000 | 530000 | sedimentation equilibrium method, gel permeation HPLC | Rhodococcus rhodochrous |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.84 | Rhodococcus rhodochrous | - |
2 kinds of enzyme, a high molecular mass: H-NHase, and a low molecular mass enzyme: L-NHase | - |
4.2.1.84 | Rhodococcus rhodochrous J1 | - |
2 kinds of enzyme, a high molecular mass: H-NHase, and a low molecular mass enzyme: L-NHase | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.1.84 | - |
Rhodococcus rhodochrous |
4.2.1.84 | ammonium sulfate fractionation, chromatography on DEAE-Sephacel, phenyl-Sepharose and Sephacryl S-300, 3.31fold purification | Rhodococcus rhodochrous |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.2.1.84 | additional information | - |
- |
Rhodococcus rhodochrous |
4.2.1.84 | 371 | - |
purified enzyme | Rhodococcus rhodochrous |
EC Number | Storage Stability | Organism |
---|---|---|
4.2.1.84 | organic acids stabilize, stable for more than 1 month in 0.1 M HEPES/KOH, pH 7.2, with 44 mM n-butyric acid, n-valeric acid, isovaleric acid, isobutyric acid or n-caproic acid | Rhodococcus rhodochrous |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.84 | 2-cyanopyridine + H2O | - |
Rhodococcus rhodochrous | pyridine-2-carbamide | - |
? | |
4.2.1.84 | 3-cyanopyridine + H2O | - |
Rhodococcus rhodochrous | pyridine-3-carbamide | i.e. nicotinamide | ? | |
4.2.1.84 | 4-cyanopyridine + H2O | - |
Rhodococcus rhodochrous | isonicotinamide | - |
? | |
4.2.1.84 | acetonitrile + H2O | best substrate | Rhodococcus rhodochrous | acetamide | - |
? | |
4.2.1.84 | acetonitrile + H2O | best substrate | Rhodococcus rhodochrous J1 | acetamide | - |
? | |
4.2.1.84 | acrylonitrile + H2O | - |
Rhodococcus rhodochrous | 2-propenoic acid amide | i.e. acrylic acid amide | ? | |
4.2.1.84 | benzonitrile + H2O | - |
Rhodococcus rhodochrous | benzoic acid amide | - |
? | |
4.2.1.84 | chloroacetonitrile + H2O | - |
Rhodococcus rhodochrous | chloroacetamide | - |
? | |
4.2.1.84 | chloroacetonitrile + H2O | - |
Rhodococcus rhodochrous J1 | chloroacetamide | - |
? | |
4.2.1.84 | crotononitrile + H2O | 19% of the activity with propionitrile | Rhodococcus rhodochrous | (E)-2-butenoic acid amide | - |
? | |
4.2.1.84 | cyanopyrazine + H2O | - |
Rhodococcus rhodochrous | pyrazincarbamide | - |
? | |
4.2.1.84 | cyclopropylcyanide + H2O | - |
Rhodococcus rhodochrous | ? | - |
? | |
4.2.1.84 | ethylene cyanhydrine + H2O | - |
Rhodococcus rhodochrous | ? | - |
? | |
4.2.1.84 | isobutyronitrile + H2O | - |
Rhodococcus rhodochrous | isobutyramide | - |
? | |
4.2.1.84 | methacrylonitrile + H2O | - |
Rhodococcus rhodochrous | methylacrylic acid amide | - |
? | |
4.2.1.84 | additional information | specificity overview | Rhodococcus rhodochrous | ? | - |
? | |
4.2.1.84 | additional information | specificity overview | Rhodococcus rhodochrous J1 | ? | - |
? | |
4.2.1.84 | n-butyronitrile + H2O | - |
Rhodococcus rhodochrous | n-butyric acid amide | - |
? | |
4.2.1.84 | n-butyronitrile + H2O | - |
Rhodococcus rhodochrous J1 | n-butyric acid amide | - |
? | |
4.2.1.84 | propionitrile + H2O | - |
Rhodococcus rhodochrous | propionic acid amide | - |
? | |
4.2.1.84 | propionitrile + H2O | - |
Rhodococcus rhodochrous J1 | propionic acid amide | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.2.1.84 | ? | x * 26000, alpha, + x * 29000, beta, SDS-PAGE | Rhodococcus rhodochrous |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.1.84 | 35 | - |
- |
Rhodococcus rhodochrous |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.1.84 | 50 | - |
stable up to, 30 min, pH 7.0, 44 mM n-butyric acid | Rhodococcus rhodochrous |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.1.84 | 6.5 | 6.8 | - |
Rhodococcus rhodochrous |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
4.2.1.84 | 5 | 8 | about 50% of activity maximum at pH 5, about 60% of activity maximum at pH 8 | Rhodococcus rhodochrous |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
4.2.1.84 | 6 | 9 | 20°C, 60 min, stable in this range, rapid loss of activity below pH 6.0 and above pH 9.0 | Rhodococcus rhodochrous |
4.2.1.84 | 6 | 9 | 25°C, 10 min, stable in this range, rapid loss of activity below pH 6.0 and above pH 9.0 | Rhodococcus rhodochrous |