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Literature summary extracted from

  • Kaakeh, M.R.; Legras, J.L.; Duran, R.; Chion, Ch.K.N.C.K.; Arnaud, A.; Galzy, P.
    Purification and properties of the nitrile hydratase of a new strain of Rhodococcus sp. (1991), Zentralbl. Mikrobiol., 146, 89-98.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.84
-
 Rhodococcus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.84 5-aminotetrazole monohydrate
-
 Rhodococcus sp.
4.2.1.84 Ca2+
-
 Rhodococcus sp.
4.2.1.84 Cu2+
-
 Rhodococcus sp.
4.2.1.84 Hg2+
-
 Rhodococcus sp.
4.2.1.84 iodoacetic acid
-
 Rhodococcus sp.
4.2.1.84 N-bromosuccinimide
-
 Rhodococcus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.84 10
-
 Isobutyronitrile pH 7, 25ºC Rhodococcus sp.
4.2.1.84 12.5
-
 acetonitrile pH 7, 25ºC Rhodococcus sp.
4.2.1.84 14.3
-
 Propionitrile pH 7, 25ºC Rhodococcus sp.
4.2.1.84 16.6
-
 acrylonitrile pH 7, 25ºC Rhodococcus sp.
4.2.1.84 33.3
-
 Benzonitrile pH 7, 25ºC Rhodococcus sp.
4.2.1.84 57
-
 pivalonitrile pH 7, 25ºC Rhodococcus sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.84 cytoplasm
-
 Rhodococcus sp. 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.84 26000
-
 x * 26000, alpha + x * 32000, beta, SDS-PAGE, strain 7 Rhodococcus sp.
4.2.1.84 32000
-
 x * 26000, alpha + x * 32000, beta, SDS-PAGE, strain 7 Rhodococcus sp.
4.2.1.84 278000
-
 gel filtration Rhodococcus sp.

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.84 Rhodococcus sp.
-
-
-
4.2.1.84 Rhodococcus sp. 7
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.84 chromatography on Sepharose Q, phenyl-Sepharose CL-4B and Sephadex G-200, strain 7 Rhodococcus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.84 additional information
-
-
 Rhodococcus sp.
4.2.1.84 3.8 4.6 with 0.5% ammonium sulfate or acetonitrile in the incubation medium Rhodococcus sp.
4.2.1.84 4.9
-
 with 0.5% acetonitrile and 0.5% yeast extract Rhodococcus sp.
4.2.1.84 485
-
 purified enzyme Rhodococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.84 acetonitrile + H2O 79% of the activity with acrylonitrile Rhodococcus sp. acetamide
-
 ?
4.2.1.84 acetonitrile + H2O 79% of the activity with acrylonitrile Rhodococcus sp. 7 acetamide
-
 ?
4.2.1.84 acrylonitrile + H2O
-
 Rhodococcus sp. 2-propenoic acid amide i.e. acrylic acid amide ?
4.2.1.84 acrylonitrile + H2O
-
 Rhodococcus sp. 7 2-propenoic acid amide i.e. acrylic acid amide ?
4.2.1.84 benzonitrile + H2O 19.4% of the activity with acrylonitrile Rhodococcus sp. benzoic acid amide
-
 ?
4.2.1.84 isobutyronitrile + H2O 62.5% of the activity with acrylonitrile Rhodococcus sp. isobutyric acid amide
-
 ?
4.2.1.84 isobutyronitrile + H2O 62.5% of the activity with acrylonitrile Rhodococcus sp. 7 isobutyric acid amide
-
 ?
4.2.1.84 pivalonitrile + H2O 62% of the activity with acrylonitrile Rhodococcus sp. 2,2-dimethylpropionic acid amide
-
 ?
4.2.1.84 pivalonitrile + H2O 62% of the activity with acrylonitrile Rhodococcus sp. 7 2,2-dimethylpropionic acid amide
-
 ?
4.2.1.84 propionitrile + H2O 64% of the activity with acrylonitrile Rhodococcus sp. propionic acid amide
-
 ?
4.2.1.84 propionitrile + H2O 64% of the activity with acrylonitrile Rhodococcus sp. 7 propionic acid amide
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.84 ? x * 26000, alpha + x * 32000, beta, SDS-PAGE, strain 7 Rhodococcus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.84 45
-
-
 Rhodococcus sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.1.84 30 60 about 60% of activity maximum at 30°C, about 50% of activity maximum at 60°C Rhodococcus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.84 50
-
 60 min, about 80% loss of activity Rhodococcus sp.
4.2.1.84 55
-
 20 min, about 90% loss of activity Rhodococcus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.84 7
-
-
 Rhodococcus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.84 5 9 about 60% of activity maximum at pH 5, about 55% of activity maximum at pH 9 Rhodococcus sp.