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Literature summary extracted from

  • Rode, H.; Giffhorn, F.
    Ferrous- or cobalt ion-dependent D-(-)-tartrate dehydratase of pseudomonads: purification and properties (1982), J. Bacteriol., 151, 1602-1604.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.81 D-malate weak Pseudomonas sp.
4.2.1.81 EDTA Fe2+ or Co2+ restores; strong Pseudomonas sp.
4.2.1.81 L(+)-Tartrate weak Pseudomonas sp.
4.2.1.81 L-malate weak Pseudomonas sp.
4.2.1.81 meso-tartrate
-
 Pseudomonas sp.
4.2.1.81 additional information no inhibition by iodoacetate Pseudomonas sp.
4.2.1.81 Zn2+
-
 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.81 0.18
-
 (S,S)-Tartrate strain Ch Pseudomonas sp.
4.2.1.81 0.6
-
 (S,S)-Tartrate strain G2 Pseudomonas sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.81 cytosol
-
 Pseudomonas sp. 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.81 Ca2+ requirement, can replace Fe2+ or Co2+ with about 30% (strain G2) and 55% (strain Ch) efficiency Pseudomonas sp.
4.2.1.81 Co2+ requirement, 0.5 mM, equally effective as Fe2+ Pseudomonas sp.
4.2.1.81 divalent cations requirement Pseudomonas sp.
4.2.1.81 Fe2+ requirement, 0.1 mM, equally effective as Co2+ Pseudomonas sp.
4.2.1.81 Mg2+ requirement, can replace Fe2+ or Co2+ with about 60% (strain G2) and 80% (strain Ch) efficiency Pseudomonas sp.
4.2.1.81 Mn2+ requirement, can replace Fe2+ or Co2+ with about 65% efficiency Pseudomonas sp.
4.2.1.81 Ni2+ requirement, can replace Fe2+ or Co2+ with about 30% (strain G2) and 80% (strain Ch) efficiency Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.81 38500
-
 2 * 38500, SDS-PAGE Pseudomonas sp.
4.2.1.81 72000
-
 strain Ch, gel filtration Pseudomonas sp.
4.2.1.81 78000
-
 strain G2, gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.81 (S,S)-tartrate Pseudomonas sp.
-
 oxaloacetate + H2O
-
 ?
4.2.1.81 additional information Pseudomonas sp. involved in D-tartrate catabolism ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.81 Pseudomonas sp.
-
 strains G2 and Ch
-

Oxidation Stability

EC Number Oxidation Stability Organism
4.2.1.81 insensitive to O2, no thiols required for protection, 15 h stable at 22°C Pseudomonas sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.81 to near homogeneity Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.81 3.1
-
 strain Ch Pseudomonas sp.
4.2.1.81 13.6
-
 strain G2 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.81 (S,S)-tartrate
-
 Pseudomonas sp. oxaloacetate + H2O
-
 ?
4.2.1.81 additional information involved in D-tartrate catabolism Pseudomonas sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.81 dimer 2 * 38500, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.81 30
-
 assay at Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.81 7.5
-
-
 Pseudomonas sp.