BRENDA - Enzyme Database

Solubilization, partial purification, and characterization of a fatty aldehyde decarbonylase from a higher plant, Pisum sativum

Schneider-Belhaddad, F.; Kolattukudy, P.; Arch. Biochem. Biophys. 377, 341-349 (2000)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
4.1.99.5
phopshatidylserine
requirement, phosphatidylcholine is preferred, phopshatidylserine and phopshatidylethanolamine substitute less effectively
Pisum sativum
4.1.99.5
phosphatidylcholine
requirement, preferred lipid, phopshatidylserine and phopshatidylethanolamine substitute less effectively
Pisum sativum
4.1.99.5
phosphatidylethanolamine
requirement, phosphatidylcholine is preferred, phopshatidylserine and phopshatidylethanolamine substitute less effectively
Pisum sativum
4.1.99.5
Phospholipid
requirement, dose-dependent stimulation, phosphatidylcholine is preferred, phopshatidylserine and phopshatidylethanolamine substitute less effectively
Pisum sativum
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.99.5
1,10-phenanthroline
1 mM, complete inhibition, CuCl2 restores, CoCl2 and ZnCl2 partially restore activity; strong
Pisum sativum
4.1.99.5
dithioerythritol
5 mM, complete inhibition
Pisum sativum
4.1.99.5
EDTA
10 mM, 58% inhibition
Pisum sativum
4.1.99.5
metal ion chelators
Cu2+ fully restores activity; strong
Pisum sativum
4.1.99.5
NADH
presence causes 50% inhibition
Pisum sativum
4.1.99.5
NADPH
presence causes 50% inhibition
Pisum sativum
4.1.99.5
O2
complete inhibition
Pisum sativum
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.1.99.5
membrane
membrane-associated
Pisum sativum
16020
-
4.1.99.5
microsome
membrane-associated
Pisum sativum
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.99.5
Cu2+
probably a copper enzyme
Pisum sativum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.99.5
67000
-
x * 67000, SDS-PAGE
Pisum sativum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.99.5
additional information
Pisum sativum
alkane biosynthesis
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.99.5
Pisum sativum
-
L. V Early Frosty
-
Oxidation Stability
EC Number
Oxidation Stability
Organism
4.1.99.5
inhibited in the presence of O2
Pisum sativum
Purification (Commentary)
EC Number
Commentary
Organism
4.1.99.5
partial, 1306fold, solubilized with beta-octyl glucoside
Pisum sativum
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.99.5
leaf
young rapidly growing
Pisum sativum
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.99.5
0.000235
-
at room temperature
Pisum sativum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.99.5
additional information
alkane biosynthesis
648395
Pisum sativum
?
-
-
-
-
4.1.99.5
octadecanal
-
648395
Pisum sativum
heptadecane + CO
-
648395
Pisum sativum
-
Subunits
EC Number
Subunits
Commentary
Organism
4.1.99.5
?
x * 67000, SDS-PAGE
Pisum sativum
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.1.99.5
additional information
-
assay at room temperature
Pisum sativum
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
4.1.99.5
phopshatidylserine
requirement, phosphatidylcholine is preferred, phopshatidylserine and phopshatidylethanolamine substitute less effectively
Pisum sativum
4.1.99.5
phosphatidylcholine
requirement, preferred lipid, phopshatidylserine and phopshatidylethanolamine substitute less effectively
Pisum sativum
4.1.99.5
phosphatidylethanolamine
requirement, phosphatidylcholine is preferred, phopshatidylserine and phopshatidylethanolamine substitute less effectively
Pisum sativum
4.1.99.5
Phospholipid
requirement, dose-dependent stimulation, phosphatidylcholine is preferred, phopshatidylserine and phopshatidylethanolamine substitute less effectively
Pisum sativum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.99.5
1,10-phenanthroline
1 mM, complete inhibition, CuCl2 restores, CoCl2 and ZnCl2 partially restore activity; strong
Pisum sativum
4.1.99.5
dithioerythritol
5 mM, complete inhibition
Pisum sativum
4.1.99.5
EDTA
10 mM, 58% inhibition
Pisum sativum
4.1.99.5
metal ion chelators
Cu2+ fully restores activity; strong
Pisum sativum
4.1.99.5
NADH
presence causes 50% inhibition
Pisum sativum
4.1.99.5
NADPH
presence causes 50% inhibition
Pisum sativum
4.1.99.5
O2
complete inhibition
Pisum sativum
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.1.99.5
membrane
membrane-associated
Pisum sativum
16020
-
4.1.99.5
microsome
membrane-associated
Pisum sativum
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.99.5
Cu2+
probably a copper enzyme
Pisum sativum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.99.5
67000
-
x * 67000, SDS-PAGE
Pisum sativum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.99.5
additional information
Pisum sativum
alkane biosynthesis
?
-
-
-
Oxidation Stability (protein specific)
EC Number
Oxidation Stability
Organism
4.1.99.5
inhibited in the presence of O2
Pisum sativum
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.99.5
partial, 1306fold, solubilized with beta-octyl glucoside
Pisum sativum
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.99.5
leaf
young rapidly growing
Pisum sativum
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.99.5
0.000235
-
at room temperature
Pisum sativum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.99.5
additional information
alkane biosynthesis
648395
Pisum sativum
?
-
-
-
-
4.1.99.5
octadecanal
-
648395
Pisum sativum
heptadecane + CO
-
648395
Pisum sativum
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.1.99.5
?
x * 67000, SDS-PAGE
Pisum sativum
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.1.99.5
additional information
-
assay at room temperature
Pisum sativum