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Literature summary extracted from
- Alkane biosynthesis by decarbonylation of aldehyde catalyzed by a microsomal preparation from Botryococcus braunii (1991), Arch. Biochem. Biophys., 287, 268-275.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.99.5 | ascorbate | 2 mM, stimulates nearly 3fold | Botryococcus braunii |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.99.5 | 1,10-phenanthroline | strong | Botryococcus braunii | |
| 4.1.99.5 | 8-hydroxyquinoline | strong | Botryococcus braunii | |
| 4.1.99.5 | EDTA | strong | Botryococcus braunii | |
| 4.1.99.5 | metal ion chelators | strong | Botryococcus braunii | |
| 4.1.99.5 | O2 | - |
Botryococcus braunii | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.99.5 | 0.065 | - |
octadecanal | 25°C | Botryococcus braunii | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 4.1.99.5 | membrane | membrane-associated | Botryococcus braunii | 16020 | - |
| 4.1.99.5 | microsome | membrane-associated | Botryococcus braunii | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.99.5 | additional information | probably a metalloenzyme | Botryococcus braunii |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.99.5 | additional information | Botryococcus braunii | final step in alkane biosynthesis | ? | - |
? | |
| 4.1.99.5 | additional information | Botryococcus braunii Austin | final step in alkane biosynthesis | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.99.5 | Botryococcus braunii | - |
belonging to the A race | - |
| 4.1.99.5 | Botryococcus braunii Austin | - |
belonging to the A race | - |
Oxidation Stability
| EC Number | Oxidation Stability | Organism |
|---|---|---|
| 4.1.99.5 | inhibited in the presence of O2 | Botryococcus braunii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.99.5 | additional information | final step in alkane biosynthesis | Botryococcus braunii | ? | - |
? | |
| 4.1.99.5 | additional information | final step in alkane biosynthesis | Botryococcus braunii Austin | ? | - |
? | |
| 4.1.99.5 | octadecanal | - |
Botryococcus braunii | heptadecane + CO | - |
? | |
| 4.1.99.5 | octadecanal | - |
Botryococcus braunii Austin | heptadecane + CO | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.1.99.5 | 25 | - |
- |
Botryococcus braunii |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.1.99.5 | 100 | - |
boiling destroys enzyme activity | Botryococcus braunii |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.99.5 | 7 | - |
very sharp | Botryococcus braunii |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.99.5 | 6 | 8 | pH 6: about 10% of activity maximum, pH 8: about 30% of activity maximum | Botryococcus braunii |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.99.5 | additional information | no cofactor requirement | Botryococcus braunii |
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Release 2023.1 (January 2023)
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